Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Isolation of Pediococcus Strain from Nuruk and Anti-Lipid Accumulation Effect of Ornithine-Containing Makgeolli on 3T3-L1 Cells

누룩으로부터 오르니틴 생성능을 갖는 Pediococcus 속 균주의 분리 및 오르니틴 함유 막걸리의 3T3-L1 세포의 중성지질 축적 억제 효과

  • Yook, Jin-Seon (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Oh, Suk-Heung (Department of Food & Biotechnolog, Woosuk University) ;
  • Kim, Su-Gon (Department of Food & Biotechnolog, Woosuk University) ;
  • Lee, Jo-Seph (Department of Food & Biotechnolog, Woosuk University) ;
  • Mun, Eun-Gyung (Dae shin Environment Development Co., Ltd., Green Bio Food Department) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, Chonbuk National University)
  • 육진선 (전북대학교 식품영양학과) ;
  • 오석흥 (우석대학교 식품생명공학과) ;
  • 김수곤 (우석대학교 식품생명공학과) ;
  • 이요셉 (우석대학교 식품생명공학과) ;
  • 문은경 ((유)대신환경개발 그린바이오식품사업부) ;
  • 차연수 (전북대학교 식품영양학과)
  • Received : 2015.06.01
  • Accepted : 2015.07.13
  • Published : 2015.09.30
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Abstract

To evaluate the functional effect of ornithine produced by isolated lactic acid bacteria, we examined the anti-lipid accumulation effect of ornithine produced by isolate lactic acid bacteria on 3T3-L1 cells. Lactic acid bacteria (Pediococcus strain) were isolated from nuruk, which is made from wheat, rice, and barley (whole grain, grits, or flour) by fermenting microorganisms (Aspergillus, Rhizopus, and yeasts). Pediococcus strain was identified by 16S rDNA sequencing analyses, and cells were collected by centrifugation and developed as an ornithine starter. makgeolli, an ornithine-containing Korean traditional alcoholic beverage, was made with isolated lactic acid bacteria and arginine. makgeolli was made with the help of ornithine starter using a makgeolli making kit. We evaluated the anti-proliferation effect of ornithine makgeolli on 3T3-L1 cells. To determine the anti-proliferation effect of ornithine makgeolli on preadipocytes, lipid droplets were quantified and stained with Oil Red O. makgeolli made with ornithine starter and arginine showed a 3-fold higher concentration of ornithine compared to makgeolli without starter and arginine. In the results of 3T3-L1 cell line experiment, lipid accumulation was significantly reduced by adding 0.05 mg/mL of ornithine makgeolli compare to the control (adipocyte without sample). In conclusion, ornithine makgeolli containing ornithine starter isolated from nuruk showed an anti-lipid accumulation effect with increased ornithine content without toxicity.

본 연구에서는 누룩에서 ornithine 생성능을 보유하고 있는 Pediococcus 속 균주를 동정하고, ornithine 생성 유산균인 Pediococcus pentosaceus TNO-4를 이용하여 막걸리를 제조하였다. Ornithine의 전구물질인 arginine과 Pediococcus pentosaceus TNO-4를 첨가하여 제조한 ornithine 함유 막걸리의 ornithine 함량을 측정하고 세포 수준에서의 지방구 형성 억제 효과를 통한 항비만 효과를 평가하였다. Ornithine 막걸리의 ornithine 함량은 일반 막걸리에 비하여 3배 증가되었으며, 세포 실험 결과 0.05 mg/mL 농도의 ornithine 막걸리를 3T3-L1 세포에 처리하였을 때 독성이 없는 수준에서 유의적으로 지방구 형성을 억제하는 것을 관찰하였다. 본 연구를 바탕으로 후속 연구가 이루어진다면 기능성 물질이 강화된 우리나라 전통술인 막걸리를 통하여 지질축적 억제를 통한 항비만 효과를 기대해 볼 수 있을 것이라 사료된다. 더 나아가서 기능성이 부각된 막걸리의 개발을 통하여 막걸리 시장에 새로운 판로를 개척할 수 있고, 누룩에서 분리된 ornithine 생성능을 가진 유산균은 막걸리 이외에 여러 가지 발효식품에도 활용될 수 있을 것으로 기대된다.

Keywords

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