Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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The Effect of Electro-acupuncture at ST36 on Carbon Tetrachloride-induced Chronic Hepatic Damage in Rats

족삼리 전침자극이 Carbon Tetrachloride 장기투여로 유발된 흰쥐의 만성 간손상에 미치는 영향

  • Kim, Byung-Soo (Department of Meridian and Acupoint, College of Korean Medicine, Daejeon University) ;
  • Lee, Hyun-Suk (Department of Meridian and Acupoint, College of Korean Medicine, Daejeon University) ;
  • Yim, Yun-Kyoung (Department of Meridian and Acupoint, College of Korean Medicine, Daejeon University)
  • 김병수 (대전대학교 한의과대학 경락경혈학교실) ;
  • 이현석 (대전대학교 한의과대학 경락경혈학교실) ;
  • 임윤경 (대전대학교 한의과대학 경락경혈학교실)
  • Received : 2015.08.13
  • Accepted : 2015.09.04
  • Published : 2015.09.27
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Abstract

Objectives : The purpose of this study is to investigate the effect of electro-acupuncture(EA) at ST36 on $CCl_4{^-}$ induced chronic hepatic damage in rats. Methods : The authors performed several experimental items, including measurements of liver index, hematological analysis for WBC, lymphocytes, neutrophils, monocytes, hemoglobin, and biochemical assays for ALT, AST, ALP in serum, and histological analysis of liver tissue. Results : Liver index(liver weight/body weight), the levels of WBC, Neutrophils and Monocytes in blood, the levels of ALT, AST and ALP in serum which were increased by $CCl_4{^-}$ intoxication were reduced significantly by EA at ST36. The levels of lymphocyte and hemoglobin in blood which were decreased by $CCl_4{^-}$ intoxication were significantly increased by EA at ST36. Conclusions : EA at ST36 has a hepatoprotective effect on chronic hepatic damage in $CCl_4{^-}$ intoxicated rats.

Keywords

References

  1. Liver System Internal Medicine Compilation Committee of Korean Oriental Medical Colleges. Liver System Internal Medicine. Seoul : Oriental Medicine Institute. 2001 ; 14-15.
  2. Plaa G. Toxic response of the liver. In: Amdur MO, Doull J, Klaassen CD. Casarett and Doull's toxicology the basic science of poisons. 4th ed. NY : Pergamon Press. 1991 ; 334-53.
  3. Gershwin ME, Vierling JM, Manns MP. Liver Immunology: Principles and Practice. Totowa, N.J: Humana Press. 2007 ; 1.
  4. Winau F, Quack C, Darmoise A, Kaufmann SHE. Starring stellate cells in liver immunology. Current Opinion in Immunology, 2008 ; 20(1) : 68-74. https://doi.org/10.1016/j.coi.2007.10.006
  5. liver System Internal Medicine Compilation Committee of Korean Oriental Medical Colleges. Liver System Internal Medicine. Seoul : Oriental Medicine Institute. 1992 ; 23-5, 254-71.
  6. Gershwin ME, Vierling JM, Manns MP. Liver Immunology: Principles and Practice. Totowa, N.J: Humana Press. 2007 ; 45.
  7. Kim G, Seo JI. drug metabolism and Drug-induced Hepatotoxicity pathogenesis. Medical Postgraduates. 2009 ; 37(4) : 191.
  8. The Acupuncture Compilation Committee. The Acupuncture and Moxibustion(Sang). Seoul : JipMoonDang. 1998 ; 382-384.
  9. Meridian & Acupoint Compilation Committee of Korean Oriental Medical Colleges. Detail of meridians & acupoints (volumeI) : a guidebook for college students. Daejeon : Jong-RyeoNaMu. 2012 ; 213-30.
  10. Choi GS, Oh SD, Han JB, Lee GS, Park JH, Bae HS, et al. Effects of Moxibustion to Zusanli(ST36) on Alteration of Natural Killer cell Activity in rat spleen. Korean Journal of Oriental Physiology & Pathology. 2002 ; 16(2) : 233-238.
  11. Kung YY, Chen FP, Hwang SJ. The different immunomodulation of indirect moxibustion on normal subjects and patients with systemic lupus erythematosus. Am J Chin Med. 2006 ; 34(1) : 47-56. https://doi.org/10.1142/S0192415X0600362X
  12. Takayama Y, Itoi M, Hamahashi T, Tsukamoto N, Mori K, Morishita D, et al. Moxibustion activates host defense against herpes simplex virus type I through augmentation of cytokine production. Microbiol Immunol. 2010 ; 54(9) : 551-7. https://doi.org/10.1111/j.1348-0421.2010.00250.x
  13. Wei ZM, Zhunag QL, Effect of Acupuncture at ST36 on leukopenia and on immunologic functions, Shanghai Journal of Acupuncture and Moxibustion, 1996 ; 15(6) : 12.
  14. Wu B. Effect of acupuncture on the regulation of cell-mediated immunity in the patients with malignant tumors. Zhen Ci Yan Jiu. 1995 ; 20(3) : 67-71.
  15. Hong YS, Hwang WJ, Kim Ks, Lee HS, Kim JS, Na CS. The effect of acupuncture at ST-36(足三里) and LI-4(合谷) on serotonin immunoreactive cells of the rat gastrointestinal tract. The Acupuncture. 1999 ; 16(3) : 379.
  16. Kim JS, Kims YS, Nam SS. The Mechanism of Immunomodulatory Effect by Electro-acupuncture in 2,4-Dinitrophenylated Keyhole Limpet Protein Immunized Mice. The Acupuncture. 2005 ; 22(3) : 23.
  17. Kim JC, Lee EY, Seo IB, Koo ST, Choi SM, Kime EH. Effects of Low Frequency Electro-Acupuncture (ST36) on the Adjuvant-Induced Arthritis in Rats. The Acupuncture. 2006 ; 23(6) : 207-219.
  18. The Acupuncture Compilation Committee. The Acupuncture and Moxibustion(Ha). Seoul : JipMoonDang. 2001 ; 1170, 1173, 1447-51.
  19. Lee JK. Theory and Clinic of Electroacupuncture. Seoul : Seo-WonDang. 1993 ; 14-7.
  20. Zhang WT, Jin Z, Cui GH, Zhang KL, Zhang L, Zeng YW, et al. Relations between brain network activation and analgesic effect induced by low vs. high frequency electrical acupoint stimulation in different subjects: a functional magnetic resonance imaging study. Brain Research. 2003 ; 982(2) : 168-178. https://doi.org/10.1016/S0006-8993(03)02983-4
  21. Park SY, Ahn SG. lumbar intervertebral disc herniation in adolescents and adults. The Acupuncture. 1995 ; 12(1) : 273-280.
  22. Illana EC. Cordyceps sinensis, a fungi used in the Chinese traditional medicine. Rev Iberoam Micol. 2007 ; 24(4) : 259-262. https://doi.org/10.1016/S1130-1406(07)70052-9
  23. Wang PY, Kaneko T, Wang Y, and Sato A. Acarbose alone or in combination with ethanol potentiates the hepatotoxicity of carbon tetrachloride and acetaminophen in rats. Hepatology. 1999 ; 29(1) : 161-5. https://doi.org/10.1002/hep.510290109
  24. Groot HD, Noll T. The crucial role of low steady state oxygen partial pressures in haloalkane free-radical-mediated lipid peroxidation: Possible implications in haloalkane liver injury. Biochem Pharmacol. 1986 ; 35(1) : 15-19. https://doi.org/10.1016/0006-2952(86)90546-0
  25. Hong SK, Lee HI. A Study on Effects of acupuncture Applied in Left and Right Posterior Limbs on Liver Damage. The journal of Jehan Oriental Medical Academy. 1998 ; 3(1) : 123-136.
  26. Liu HJ, Hsu SF, Hsieh CC, Ho TY, Hsieh CL, Tsai CC, Lin JG. The effectiveness of Tsu-San-Li(St-36) and Tai-Chung(Li-3) acupoints for treatment of acute liver damage in rats, Am J Chin Med. 2001 ; 29(2) : 221-6. https://doi.org/10.1142/S0192415X01000253
  27. Youn DH, Na CS. Hepatoprotective effects of Electro-Acupuncture at Taechung (LR3) and Yangji (TE4) on experimental Liver injury in rats. Korean Journal of Acupuncture. 2006 ; 23(2) : 167-176.
  28. The Korean Association of Internal Medicine. Harrison's Principles of Internal Medicine. Seoul : MIP Publishing Co. 2006 ; 1970-1.
  29. Koh JI, Koh EB, Kim JH, Kim HD, Park BL, Park YS, et al. Human physiology. Seoul. TamGuDang. 1993 ; 525-6.
  30. Kim JJ, Kim BU. Wu HK, Kim DH. Liver System Oriental Internal Medicine. Seoul : JipMoonDang. 1986 ; 27-36.
  31. Na CS, Honh S, Kim HC. Effects of Moschus, Bovis Calculus, Ursi Fel Aqua-acupuncture on Liver Damage. Journal of Korean Medicine. 1996 ; 17(2) : 251-263.
  32. Kim JJ, Kim BU. Wu HK, Kim DH. Liver System Oriental Internal Medicine. Seoul : JipMoonDang. 1986 ; 27-36.
  33. Lee JK. Theory and Clinic of Electroacupuncture. Seoul : SeoWonDang. 1993 ; 14-7.
  34. The Acupuncture Compilation Committee. The Acupuncture and Moxibustion(Ha). Seoul : JipMoonDang. 2001 ; 1170, 1173, 1447-51.
  35. Sohn SS, Nam SS, Lee JD, Choi DY, Ahn BC, Park DS, Lee YH, Choi YT. Effect of stimulation - duration of high frequency electroacupuncture on the neuronal activities in the spinal cord and brainstem using Fos immunohistochemical technique. The Acupuncture. 1998 ; 15(2) : 17-28.
  36. Sohn YJ, Won R, Jung HS, Kim YS, Park YB, Sohn NW. 2-DG Autoradiographic Imaging of Brain Activity Patterns by Electroacupuncture Stimulation in Awake Rats. The Acupuncture. 2001 ; 18(3) : 56-68.
  37. Cho JH, Sin JS, Kim EJ, Shin SH, Jang JY, Shin KS, et al. Protective Effect of Lycii fructus Extract against Hepatotoxicity induced by Carbon Tetrachloride. The Korean Journal of Laboratory Animal Science. 2004 ; 20(2) : 187-93.
  38. Yao HW, Li J, Chen JQ, Xu SY. Inhibitory effect of leflunomide on hepatic fibrosis induced by CCI4 in rats. Acta Pharmacol Sin. 2004 ; 25(7) : 915-920.
  39. Chenoweth MB, Hake CL. The Smaller Halogenated Aliphatic Hydrocarbons. Annual Review of Pharmacology. 1962 ; 2(1) : 363-398. https://doi.org/10.1146/annurev.pa.02.040162.002051

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