Korean Journal of Environment and Ecology (한국환경생태학회지)

Korean Society of Environment and Ecology (한국환경생태학회)
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Effect of Salinity on the Survival and Growth of Larvae of the Boreal Digging Frog (Kaloula borealis)

맹꽁이 유생의 생장과 생존에서의 염분영향

  • Received : 2015.07.31
  • Accepted : 2015.08.17
  • Published : 2015.08.30
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Abstract

This study was conducted to examine the effect of salinity on the early embryonic development stage of boreal digging frogs (Kaloula borealis). An experiment was carried out with the eggs of K. borealis mating couples laid along the coastal zone of Jeju Island in June 2013. The salinity was set to 0, 1, 3, 5, and 7 ‰, respectively. The survival and growth of the larvae at various stages from the egg phase to the closing of their external gills phase were observed. In another experiment, tadpoles in internal gill stage were placed in solutions with salinity of 0, 1, 3, 5, and 7 ‰, respectively, and their survival rate and growth rate were observed, and then the results of the two experiments above were compared. The results showed that in egg and external gill stage, there was no significant difference in survival rate in 3 ‰ solution, though there was low survival rate in 5 ‰ solution. Further, all the eggs were dead in 7 ‰ solution, so it appeared that solutions with salinity of 5 ‰ or higher affect the survival of K. borealis in the early embryonic development. Larvae in the internal gill stage showed no significant difference in survival rate from the control group up to a salinity of 5 ‰, but when placed in a solution of over 7 ‰ salinity, the survival rate decreased. The growth rate also slowed down with the higher salinity. It appeared that in both the external gill stage and the internal gill stage in 5 ‰ solution, the growth rate decreased significantly compared to the control group. From this study it is concluded that higher salinity values have a significant impact on the survival and growth of the K. borealis larvae and this finding can be used to conserve K. borealis species that are decreasing in number due to the side effects of development in coastal areas.

우리는 염분농도가 맹꽁이 초기발생과정에 미치는 영향을 알아보고자 실시하였다. 연구는 2013년 6월에 제주지역 해안가에서 산란하는 맹꽁이 포접쌍의 알을 가지고 실시하였다. 염분농도는 0, 1, 3, 5, 7‰로 하여 알에서 외부아가미가 닫힐 때까지 생존여부와 생장을 관찰하였다. 또 다른 실험은 내부아가미 단계인 올챙이를 0, 1, 3, 5, 7‰의 염분농도에 넣어 생존율과 생장률을 관찰하여 두 실험결과를 비교하였다. 실험결과 알 - 외부아가미 단계에서는 3‰에서 대조군과 유의한 차이가 없이 생존하였으나 5‰에서는 생존율이 낮게 나타났다. 또한 7‰에서는 모든 알이 죽는 것으로 나타나 염분농도가 5‰이상에서는 맹꽁이 초기발생에 생존 영향을 끼치는 것으로 나타났다. 내부아가미 단계에서의 유생들은 5‰까지는 대조군과 유의한 차이가 없었으나 7‰에서는 생존율이 감소하였다. 생장률인 경우도 염분의 농도가 높아질수록 생장이 느려지고 있었고 외부아가미 단계 및 내부아가미 단계 모두 5‰에서 생장률이 대조군과 유의하게 감소하는 것으로 나타나고 있었다. 이번 연구 결과 염분의 농도가 높을수록 맹꽁이 유생의 생존과 생장에 영향을 받는다는 것을 알 수 있었으며, 해안지역 개발에 따른 부작용인 맹꽁이의 감소에 대한 종 보존에 대비 할 수 있을 것으로 판단된다.

Keywords

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