Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Temperature dependent 2,3-dihydroxybenzoic acid production in Acinetobacter sp. B-W

Acinetobacter sp. B-W의 온도 의존적 2,3-dihydroxybenzoic acid 생산

  • Kim, Kyoung-Ja (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Lee, Jae-Hun (Department of life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Yang, Yong-Joon (Department of Plant and Food Science, Sangmyung University)
  • 김경자 (순천향대 생명시스템학과) ;
  • 이재훈 (순천향대 생명시스템학과) ;
  • 양용준 (상명대학교 식물식품공학과)
  • Received : 2015.08.06
  • Accepted : 2015.08.26
  • Published : 2015.09.30
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Abstract

A soil microorganism producing iron chelator (siderophore) under low iron stress (up to $2{\mu}M$ of iron) was identified as Acinetobacter sp. B-W by 16S rDNA sequence analysis, biochemical-, physiological tests and morphological analysis using electron microscope. Catechol nature of siderophore was detected by Arnow test. Although optimal cell growth was identified at $36^{\circ}C$ in iron-limited media, significant quantities of siderophore were produced only at $28^{\circ}C$. Biosynthesis of siderophore was strongly inhibited by growth at $36^{\circ}C$. Production of siderophore was completely inhibited by $10{\mu}M\;FeCl_3$. Iron chelator produced from Acinetobacter sp. B-W was purified from supernatant using butanol extraction, Sephadex LH-20 column chromatography and HPLC. Purified sideropore was identified as 2,3-dihydroxybenzoic acid by HPLC, TLC and IR analysis.

철 스트레스($2{\mu}M$ 이하 농도) 하에서 시데로포어를 생산하는 균주를 토양에서 분리하여 16S rDNA 염기 서열 분석과 생화학적, 생리학적 분석 및 전자 현미경 관찰 등으로 동정한 결과, Acinetobacter sp.로 밝혀졌다. 시데로포어의 카테콜 특성은 Arnow법으로 조사되었다. 철을 제한한 배지에서 균주를 배양한 결과, $36^{\circ}C$에서도 잘 자랐지만 시데로포어 생산은 $28^{\circ}C$에서 높았다. $36^{\circ}C$에서는 시데로포어 생산이 강하게 억제되었다. $10{\mu}M\;FeCl_3$를 첨가한 배지에서는 시데로포어 생산이 완전히 억제되었다. 균주 상등액을 부탄올 추출 후, Sephadex LH-20 컬럼 크로마토그래피와 HPLC를 이용하여 시데로포어를 분리, 정제하였다. 분리, 정제된 시데로포어의 구조는 HPLC, TLC와 IR 분석 결과로부터 2,3-dihydroxybenzoic acid로 확인되었다.

Keywords

References

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