Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
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The CmACS-7 Gene Provides Sequence Variation for Development of DNA Markers Associated with Monoecious Sex Expression in Melon (Cucumis melo L.)

  • Kim, Nahui (Department of Horticultural Bioscience, Pusan National University) ;
  • Oh, Juyeol (Gyeongnam Agricultural Research & Extension Services) ;
  • Kim, Bichseam (Department of Horticultural Bioscience, Pusan National University) ;
  • Choi, Eung Kyu (Jangchun Seed Company) ;
  • Hwang, Un Sun (Jangchun Seed Company) ;
  • Staub, Jack E. (U.S. Department of Agriculture, Agricultural Research Service, Forage and Range Research Laboratory) ;
  • Chung, Sang-Min (Department of Life Science, Dongguk University-Seoul) ;
  • Park, Younghoon (Department of Horticultural Bioscience, Pusan National University)
  • Received : 2015.02.12
  • Accepted : 2015.08.02
  • Published : 2015.08.31
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Abstract

Most melon (Cucumis melo L.) breeding lines in South Korea display andromonoecious sex expression, which necessitates laborious hand emasculation during F1 hybrid seed production. Thus, there is a need to develop monoecious sex types in elite germplasm to obviate self-pollination. Sex expression is associated with floral ethylene production, which, in monecious melon plants, is associated with the A locus. Our study was conducted to develop molecular markers for selection of monoecious plants based on sequence variation inherent in the CmACS-7 gene [encoding 1-aminocyclopropane-1-carboxylic acid synthase (ACS)] that is associated with ethylene production. Full-length CmACS-7 sequences were cloned from a monoecious (MO23) and an andromonoecious (AM24) line. The alignment of those CmACS-7 sequences revealed a single nucleotide polymorphism (SNP; C170T) in exon 1 and an 18 bp indel in the 3'-untranslated region (UTR) of between MO23 and AM24, which was then used to develop a cleaved amplified polymorphic sequence (CAPS) (EX1-C170T) and a sequence characterized amplified region (SCAR) marker (T1ex), respectively. The sex expression and the T1ex SCAR-based genotype of 442 $F_2$ plants derived from a $MO23{\times}AM24$ cross was determined. Monoecy and andromonoecy segregated in a 3:1 ratio in $F_2$ progeny, where the sex type of 429 plants (13 plants not classified) co-segregated with the SCAR marker, demonstrating that sex expression regulated by CmACS-7 is controlled by a single dominant gene and that it confers monoecy in line MO23. Allelic variation in 112 geographically diverse melon lines for CmACS-7 as accessed by CAPS EX1-C170T and SCAR T1ex markers indicated that the: 1) exon 1 of CmACS-7 is highly conserved and the SNP/sex expression association detected is highly predictable making it potentially useful for marker-based selection of monoecious plants, and; 2) 18 bp indel mutation in the 3'-UTR was present in various lengths depending on different monoecious melon germplasm.

Keywords

Acknowledgement

Supported by : Ministry of Agriculture , Food and Rural Affairs (MAFRA), Ministry of Oceans and Fisheries (MOF), Rural Development Administration (RDA), Korea Forest Service (KFS)

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