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Effects of LED Light Conditions on Growth and Analysis of Functional Components in Buckwheat Sprout

LED 광 조건에 따른 메밀 새싹의 생육 및 기능성 물질 분석

  • Jeon, A-Young (Department of Crop Science, Chungbuk National University) ;
  • Kim, Ki-Hyun (Department of Crop Science, Chungbuk National University) ;
  • Kwon, Soo-Jeong (Department of Crop Science, Chungbuk National University) ;
  • Roy, Swapan Kumar (Department of Crop Science, Chungbuk National University) ;
  • Cho, Seong-Woo (Crop Breeding Research Division, NICS) ;
  • Woo, Sun-Hee (Department of Crop Science, Chungbuk National University)
  • 전아영 (충북대학교 식물자원학과) ;
  • 김기현 (충북대학교 식물자원학과) ;
  • 권수정 (충북대학교 식물자원학과) ;
  • ;
  • 조성우 (농촌진흥청 국립식량과학원) ;
  • 우선희 (충북대학교 식물자원학과)
  • Received : 2015.09.04
  • Accepted : 2015.09.14
  • Published : 2015.09.30

Abstract

Buckwheat sprouts are a vegetable; a functional food should provide health benefit and enhance performance as high nutritionally important substances. Buckwheat noodles are the major buckwheat food in Japan, Korea and China. In addition, Buckwheat as preventive medicine has undergone a great advancement in the last decade. Comparison of the functional properties distribution and utilization in tatary buckwheat is required of understanding the metabolites. The study was conducted to identify the sorts of phenolic compounds and metabolites in tatary buckwheat seedling at 4, 7, and 10 days seedling under the different combinations of light-emitting diode (LED) such as blue, red, mix (red, blue, and white), dark, and natural lights in stem and leaves. After breaking the dormancy, buckwheat seeds were grown in culture room under lights for 14 hrs and the dark condition for 10 hrs, at $25^{\circ}C$ for 10 days. Length of buckwheat was gradually increased under all of the conditions. Using HPLC, rutin was highest at 7 days under mix and natural light in stem and leaf, respectively. Quercetin was highest at 4 and 7 days under natural light in both. Chlorogenic acid was highest at 7 days under mix and natural in stem and leaf, respectively. Taken Together, this study indicates that phenolic compounds and metabolites present in those plants could be helpful for the human health and nutritional additive.

메밀 새싹 채소 재배 시 광 조건에 따른 생육 차이는 일반 메밀 새싹과 쓴 메밀 새싹의 품종에 관계없이 암 조건, 적색광, 청색광, 혼합광(청색 + 적색 + 백색), 자연광 순으로 나타났다. 루틴의 함량 차이는 메밀 새싹에서 루틴 함량차이는 일반 메밀 새싹 보다 쓴 메밀 새싹에서 더 높았다. 일반 메밀 새싹과 쓴 메밀 새싹 모두에서 자연광, 혼합광(청색 + 적색 + 백색), 청색광, 적색광, 암조건 순으로 나타났다. 자엽과 배축의 경우에는 자엽에서 배축보다 6배 가량 높게 나타났다. 퀘르세틴 함량은 일반 메밀 새싹에 비해 쓴 메밀 새싹의 함량이 10배 이상 높게 나타났다. 광 조건에 따른 함량 차이는 자연광에서 가장 높았고 다음 혼합광, 적색광, 청색광 순이고 암 조건에서 가장 낮게 나타났다. 자엽과 배축의 경우에는 자엽에서 배축보다 높은 함량을 나타내었다. 메밀 새싹 채소의 클로로겐산은 일반 메밀 새싹과 쓴 메밀 새싹의 함량 차이가 비교적 작았다. 광 조건에 따른 차이는 자연광에서가장 높았고 LED광에서는 혼합광, 청색광, 적색광 순으고 암 조건에서 가장 낮았다. 루틴과 퀘르세틴과는 다르게 클로로겐산의 경우에는 배축에서 더 많은 함량을 나타내었다.

Keywords

References

  1. Cho, J. Y., D. M. Son, J. M. Kim, B. S. Seo, S. Y. Yang, B. W. Kim, and B. G. Heo. 2008. Effects of Various LEDs on the Seed Germination, Growth Physiological Activities of Rape (Brassica napus) Sprout Vegetable, Korean J. Plant Res. 21(4) : 304-309.
  2. Choi, B. H. 1992. Status of buckwheat genetic resources in East Asia 1991. Korean J. Breed, 24 : 293-301.
  3. Clifford, M. N. 2000. Chlorogenic acid and other cinnamatesnature, occurrence, dietary burden, absorption and metabolism. J. Sci. Food Agric. 80 : 1033-1043. https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<1033::AID-JSFA595>3.0.CO;2-T
  4. Ikeda, S, T. Yamashita, T. Murakami. 1995. Minerals in buckwheat. In Current Advances in Buckwheat Researcheditied by Matano T, Ujihara A. pp. 789-792. Shinshu University Press, Asahi Matsumoto City.
  5. Jin, E. Y., Y. S. Park, J. K. Jang, M. S. Chung, H. Park, K. S. Shim, and Y. J. Choi. 2009. Extraction of quercetin and its glucosides from onion edible part using solvent extractoin and various extraction assisting methods. Food Eng Prog 13 : 147-153.
  6. Kreft, I., K. J. Chang, Y. S. Choi, and C. H. Park. 2003. Ethnobotany of buckwheat. Jinsol Publishing Co. Seoul.
  7. Lee, M. K., S. H. Park, and S. J. Kim. 2011. A time-course study of flavonoids in buckwheats (Fagopyrum species). Journal of Agricultural Science 38 : 87-94.
  8. Lee, J. G., S. S. Oh, S. H. Cha, Y. A. Jang, S. Y. Kim, Y. C. Um, and S. R. Cheong. 2010. Effects of Red/Blue Light Ratio and Short-term Light Quality Conversion on Growth and Anthocyanin Contents of Baby Leaf Lettuce. Journal of Bio-Environment Control 19(4) : 351-359.
  9. Margna, U. E. Margna. 1978. Differential biosynthesis of buckwheat flavonoids from endogenous substrates. Biochem. Physiol. Pflanzen 173 : 347-354.
  10. Mizuno, M., H. Tsuchida, N. Kozukue, and S. Mizuno. 1992. Rapid quantitative analysis and distribution of free quercetin in vegetables and fruits. Nippon shokuhin kogyo gakkaishi, 39 : 88. https://doi.org/10.3136/nskkk1962.39.88
  11. Park, B. J., J. I. Park, K. J. Chang, and C. H. Park. 2005. Comparison in Rutin Content of Tartary Buckwheat (Fagopyrum tataricum). Korean J. Plant Res. 18(2) : 246-250.
  12. Pomeranz, Y. 1983. Buckwheat: structure, composition and utilization. CRC Crit. Rev. Food Sci. Nutr. 19 : 213-258. https://doi.org/10.1080/10408398309527376
  13. Rodriguez de Sotillo, D., M. Hadley, and C. Wolf-hall. 1998. Potato peel extract anonmutagenic antioxidant with potential antimicrobial activity. J. Food Sci. 63 : 907-910. https://doi.org/10.1111/j.1365-2621.1998.tb17924.x
  14. Sharma, R. R., A. Demirci, L. R. Beuchat, and W. F. Pett. 2002. Inactivation of Escherichia coli O157:H7 on inoculated alfalfa seeds with ozonated water and heat treatment. J. Food Prot. 65 : 447-451.
  15. Watanabe, M., M. Ito. 2002. Changes in antioxidative activity and flavonoids composition of the extracts from aerial parts of buckwheat during growth period. Nippon Shokuhin Kagaku Kogaku Kaishi 49 : 119-125. https://doi.org/10.3136/nskkk.49.119
  16. Yoon, Y. H., J. G. Lee, J. C. Jeong, D. C. Jang, and C. S. Park. 2009. The effect of temperature and light conditions on growth and antioxidant contents of tatary buckwheat sprout, The Proceeding of the ISBS, Korea 54-59.

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