Journal of Neurogastroenterology and Motility

The Korean Society of Neurogastroenterology and Motility (대한소화기 기능성질환 운동학회)
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The Effects of an Extract of Atractylodes Japonica Rhizome, SKI3246 on Gastrointestinal Motility in Guinea Pigs

  • Park, Jae Jun (Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Chon, Nu Ri (Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Lee, Young Ju (Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine) ;
  • Park, Hyojin (Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine)
  • Received : 2014.09.24
  • Accepted : 2015.03.15
  • Published : 2015.07.30
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Abstract

Background/Aims There are limited therapeutic options available for irritable bowel syndrome with diarrhea (IBS-D). We tested the effects of Atractylodes japonica rhizome, a perennial plant native to North Asia, on both upper and lower gastrointestinal (GI) motility in guinea pigs. Methods The extract of A. japonica rhizome was administered orally at different doses to test its effects on upper GI motility as determined from charcoal transit in native guinea pigs and in guinea pigs pretreated with thyrotropin-releasing hormone or mustard oil. Regarding its effect on lower GI motility, the removed guinea pig colon was suspended in a chamber containing Krebs-Henseleit solution and the transit time of artificial feces was measured with various dilutions of the extract. As for in vivo assay, weight and number of fecal pellets expelled were determined under the same drug preparation used in upper GI motility experiment. Results The extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states. However, the extract increased colonic transit time in the in vitro model. In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts. In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were significantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract. Conclusions Our findings suggest that the extract of A. japonica rhizome can be a potential agent for IBS-D.

Keywords

Acknowledgement

Supported by : SK Chemical Company

References

  1. Son HJ, Jung K, Park YH, et al. Inhibitory effects of SKI3246, the rhizome extract of Atractylodes japonica, on visceral hypersensitivity in experimental irritable bowel syndrome rat models. Arch Pharm Res 2015;38:642-649. https://doi.org/10.1007/s12272-014-0454-x
  2. Kojima R, Doihara H, Nozawa K, Kawabata-Shoda E, Yokoyama T, Ito H. Characterization of two models of drug-induced constipation in mice and evaluation of mustard oil in these models. Pharmacology 2009;84:227-233. https://doi.org/10.1159/000236524
  3. Park YM, Lee YJ, Lee YH, Kim TI, Park H. Effects of ramosetron on gastrointestinal transit of Guinea pig. J Neurogastroenterol Motil 2013;19:36-41. https://doi.org/10.5056/jnm.2013.19.1.36
  4. Waterman SA, Tonini M, Costa M. The role of ascending excitatory and descending inhibitory pathways in peristalsis in the isolated guinea-pig small intestine. J Physiol 1994;481(Pt 1):223-232. https://doi.org/10.1113/jphysiol.1994.sp020433
  5. Ji SW, Park H, Chung JP, Lee SI, Lee YH. Effects of tegaserod on ileal peristalsis of guinea pig in vitro. J Pharmacol Sci 2004;94:144-152. https://doi.org/10.1254/jphs.94.144
  6. Kimball ES, Wallace NH, Schneider CR, D'Andrea MR, Hornby PJ. Small intestinal cannabinoid receptor changes following a single colonic insult with oil of mustard in mice. Front Pharmacol 2010;1:132.
  7. Kimball ES, Palmer JM, D'Andrea MR, Hornby PJ, Wade PR. Acute colitis induction by oil of mustard results in later development of an IBS-like accelerated upper GI transit in mice. Am J Physiol Gastrointest Liver Physiol 2005;288:G1266-G1273. https://doi.org/10.1152/ajpgi.00444.2004
  8. Amann R, Egger T, Schuligoi R. The tachykinin NK1 receptor antagonist SR140333 prevents the increase of nerve growth factor in rat paw skin induced by substance P or neurogenic inflammation. Neuroscience 2000;100:611-615. https://doi.org/10.1016/S0306-4522(00)00315-8
  9. Inoue H, Asaka T, Nagata N, Koshihara Y. Mechanism of mustard oil-induced skin inflammation in mice. Eur J Pharmacol 1997;333:231-240. https://doi.org/10.1016/S0014-2999(97)01040-6
  10. Louis SM, Jamieson A, Russell NJ, Dockray GJ. The role of substance P and calcitonin gene-related peptide in neurogenic plasma extravasation and vasodilatation in the rat. Neuroscience 1989;32:581-586. https://doi.org/10.1016/0306-4522(89)90281-9
  11. Bond EF, Heitkemper MM, Gruver MK. Mediation of thyrotropin-releasing hormone induced gastric motility increases in developing rats. Eur J Pharmacol 1992;217:127-135. https://doi.org/10.1016/0014-2999(92)90831-N
  12. Bond E, Heitkemper M. Thyrotropin-releasing hormone stimulates intestinal transit in young rats. Regul Pept 1990;27:263-271. https://doi.org/10.1016/0167-0115(90)90045-X
  13. Smith JR, La Hann TR, Chesnut RM, Carino MA, Horita A. Thyrotropin-releasing hormone: stimulation of colonic activity following intracerebroventricular administration. Science 1977;196:660-662. https://doi.org/10.1126/science.404705
  14. Morley JE, Steinbach JH, Feldman EJ, Solomon TE. The effects of thyrotropin releasing hormone (TRH) on the gastrointestinal tract. Life Sci 1979;24:1059-1065. https://doi.org/10.1016/0024-3205(79)90038-9
  15. Miyata K, Kamato T, Nishida A, et al. Role of the serotonin3 receptor in stress-induced defecation. J Pharmacol Exp Ther 1992;261:297-303.
  16. Oouchi M, Ichikawa S. Thyrotropin-releasing hormone effects on guinea pig antrum. Gastroenterology 1985;88(5 Pt 1):1126-1131. https://doi.org/10.1016/S0016-5085(85)80070-6

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