Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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Study on Convergence Technique Using the Antimicrobial Resistance and Virulence Genes Analysis in Escherichia coli

대장균의 항균제 내성과 독력 유전자의 분석을 활용한 융합기술연구

  • Han, Jae-Il (Department of Public Health, Graduate School of Dankook University) ;
  • Sung, Hyun-Ho (Department of Clinical Pathology, Dongnam University) ;
  • Park, Chang-Eun (Department of Biomedical Laboratory Science, Namseoul University)
  • 한재일 (단국대학교 일반대학원 보건학과) ;
  • 성현호 (동남보건대학교 임상병리과) ;
  • 박창은 (남서울대학교 임상병리학과)
  • Received : 2015.08.17
  • Accepted : 2015.10.20
  • Published : 2015.10.31
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Abstract

This study was conducted to investigate the characteristics of antibiotic resistant E. coli. its antibiotic susceptibility and pathogenicity were analyzed via molecular convergence technique, for the relationship of antibiotic susceptibility and pathogenicity. The 60 isolated strains consisted of ESBL(+)(8) and ESBL(-)(52) strains. The ESBL(+)(8) strains consisted of 2 strains without a pathogenic gene, stb(3), flich7(1), and flich7-eae(2). The ESBL(-)(52) strains consisted of 26 strains without a pathogenic gene, stx1(3), stb(10), flich7(2), eae(2), stx1-flich7(2), stx1-stb(4), flich7-stb(2), and flich7-stb-eae(1). In conclusion, antibiotic resistance is increasingly, Focused on molecular convergence, showed the correlation of pathogenicity with antibiotic resistance was poor. However, It will be able to find the exact pathogenic factor in the future through convergence technique including the analysis of virulence genes.

본 연구는 항균제에 내성을 보이는 대장균의 특성을 알아보기 위해 설사환자에서 분리된 대장균에 대한 항균제 감수성 및 병원성 인자의 상관성을 분자융합적 기술을 통해 조사하였다. 분리한 대장균의 항균제 내성은 60주에서 ESBL(extendede spectrum ${\beta}$-lactamase) positive균주가 8주이고, negative균주는 52주였다. ESBL 양성 8주 중 2주는 병원성 유전자가 검출되지 않았으며, stb(3주), flich7(1주), flich7-eae(2주)로 나타났다. ESBL 음성 52주 중 26주는 병원성 유전자가 검출되지 않았고, stx1(3주), stb(10주), flich7 및 eae(각 2주), stx1-flich7(2주), stx1-stb(4주), flich7-stb(2주), flich7-stb-eae(1주)이었다. 결론적으로 항균제 내성이 증가하는 시대에 분자 융합적 관점에서 독력 유전자의 분포와 항균제 내성과의 관계는 적게 나타났으나, 향후 다양한 독력 유전자의 분석을 통한 융합기술연구가 이루어진다면 보다 정확한 병원성 인자를 추정할 수 있을 것으로 사료된다.

Keywords

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