Yonsei Medical Journal

Yonsei Univ College Medicine (연세대학교의과대학)
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Hypoxia Increases Epithelial Permeability in Human Nasal Epithelia

  • Min, Hyun Jin (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Kim, Tae Hoon (The Airway Mucus Institute, Yonsei University College of Medicine) ;
  • Yoon, Joo-Heon (Department of Otorhinolaryngology, Yonsei University College of Medicine) ;
  • Kim, Chang-Hoon (Department of Otorhinolaryngology, Yonsei University College of Medicine)
  • Received : 2014.05.21
  • Accepted : 2014.08.22
  • Published : 2015.05.01
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Abstract

Purpose: The nasal mucosa is the first site to encounter pathogens, and it forms continuous barriers to various stimuli. This barrier function is very important in the innate defense mechanism. Additionally, inflammation of the nasal sinus is known to be a hypoxic condition. Here, we studied the effect of hypoxia on barrier function in normal human nasal epithelial (NHNE) cells. Materials and Methods: The expression levels of various junction complex proteins were assessed in hypoxia-stimulated NHNE cells and human nasal mucosal tissues. We performed real-time polymerase chain reaction analysis, western blotting, and immunofluorescence assays to examine differences in the mRNA and protein expression of ZO-1, a tight junction protein, and E-cadherin in NHNE cells. Moreover, we evaluated the trans-epithelial resistance (TER) of NHNE cells under hypoxic conditions to check for changes in permeability. The expression of ZO-1 and E-cadherin was measured in human nasal mucosa samples by western blotting. Results: Hypoxia time-dependently decreased the expression of ZO-1 and E-cadherin at the gene and protein levels. In addition, hypoxia decreased the TER of NHNE cells, which indicates increased permeability. Human nasal mucosa samples, which are supposed to be hypoxic, showed significantly decreased levels of ZO-1 and E-cadherin expression compared with control. Conclusion: Our results demonstrate that hypoxia altered the expression of junction complex molecules and increased epithelial permeability in human nasal epithelia. This suggests that hypoxia causes barrier dysfunction. Furthermore, it may be associated with innate immune dysfunction after encountering pathogens.

Keywords

Acknowledgement

Supported by : National Research Foundation (NRF)

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