Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Neuronal Cell Protective Effect of New Green Extract against H2O2-induced Oxidative Stress and Analysis of Bioactive Compounds

과산화수소(H2O2)로 유도된 산화 스트레스에 대한 뉴그린 추출물의 신경세포 보호효과 및 생리활성물질 분석

  • Ha, Jeong Su (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Chang Hyeon (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Seung, Tae Wan (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Guo, Tian Jiao (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kang, Jin Young (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Du Sang (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Uk (Department of Special Purpose Trees, Korea Forest Research Institute) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 하정수 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 박선경 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 박창현 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 승태완 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 궈텐자오 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 강진용 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 이두상 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 김종민 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원) ;
  • 이욱 (산림과학원 특용자원연구과) ;
  • 허호진 (경상대학교 응용생명과학부(BK21 plus) 농업생명과학연구원)
  • Received : 2015.08.04
  • Accepted : 2015.09.25
  • Published : 2015.10.31
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Abstract

In vitro antioxidant activities and neuronal cell protective effects of the ethyl acetate fraction of a new green extract (Brassica oleracea var. botytis aut italiana) against $H_2O_2$-induced oxidative stress were investigated, and its industrial feasibility was evaluated. The extract showed the highest contents of total phenolic compounds among other extracts as well as a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and malondialdehyde (MDA) inhibitory effect. This extract not only decreased the intracellular reactive oxygen (ROS) level but also protected the neuronal cells against $H_2O_2$-induced oxidative stress. On analysis using gas chromatograph-mass spectrometry, the following phenolic compounds were identified: quinic acid, ferulic acid, and caffeic acid. Collectively, these results suggest that this new green extract could contain functional substances that would help prevent the risk of neurodegenerative disease.

본 연구에서는 뉴그린(Brassica oleracea var. botytis aut italiana)의 산화방지 효과와 신경세포 보호효과 및 주요 생리활성물질을 분석하였다. 뉴그린 아세트산에틸 분획물은 다른 분획물보다 총 페놀 함량(178.83 mg GAE/g of dried new green)이 높게 나타났으며, 이에 따라 아세트산에틸 분획물로 in vitro 산화방지 실험을 한 결과 상대적으로 우수한 ABTS 라디칼 소거활성과 말론다이알데하이드 생성 억제효과를 나타내었다. 신경세포에 과산화수소로 유발시킨 산화 스트레스에 대한 뉴그린 아세트산에틸 분획물은 산화 스트레스의 생성을 억제하고 세포 생존율을 향상시킬 뿐만 아니라 신경 세포막 손상을 보호하는 것으로 나타났다. 이러한 효과를 나타내는 뉴그린 아세트산에틸 분획물의 주요 생리활성 물질을 확인하기 위해 가스크로마토그래프질량분석을 실시한 결과 퀸산, 페룰산 및 카페산이 존재하는 것으로 확인되었다. 본 연구 결과를 바탕으로, 뉴그린 아세트산에틸 분획물은 우수한 산화방지 효과와 신경세포 보호효과를 나타냄에 따라, 퇴행성 신경질환과 같은 질병을 예방할 수 있는 천연물 유래의 기능성 소재로서의 활용가치가 있다고 판단된다.

Keywords

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