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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Cell Cycle Arrest Effects by Artemisia annua Linné in Hep3B Liver Cancer Cell

Hep3B 간암세포에서 개똥쑥 추출물에 의한 Cell Cycle Arrest 효과

  • Kim, Eun Ji (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Guen Tae (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Bo Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Lim, Eun Gyeong (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Sang Yong (Department of BIONGENE) ;
  • Ha, Sung Ho (Department of Chemical Engineering, College of Life Science and Nano Technology, Hannam University) ;
  • Kim, Young Min (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University) ;
  • Yoo, Je-Geun (Department of Biological Sciences and Biotechnology, College of Life Science and Nano Technology, Hannam University)
  • 김은지 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김근태 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김보민 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 임은경 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 김상용 ((주)바이오앤진) ;
  • 하성호 (한남대학교 생명나노과학대학 화학공학과) ;
  • 김영민 (한남대학교 생명나노과학대학 생명시스템과학과) ;
  • 유제근 (한남대학교 생명나노과학대학 생명시스템과학과)
  • Received : 2015.06.22
  • Accepted : 2015.08.17
  • Published : 2015.08.27
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Abstract

Cells proliferate via repeating process that growth and division. This process is G1, S, G2 and M four phases consists. Monitoring the progression of the cell cycle is a specific step that to be a continuous process is repeated to adjust the start of the next step. At this time, this process is called a Checkpoint. Currently, there are three known checkpoints that G1-S phase, G2-M phase, and the M phase. In this study, we confirmed that cell cycle arrest effects by ethanol extracts of Artemisia annua Linne (AAE) in Hep3B liver cancer cells. AAE was regulated proteins which involved in cell cycle such as pAkt, pMDM2, p53, p21, pCDK2 (T14/Y15). AAE induced cell cycle arrest in G1 checkpoint through phosphorylation of CDK2. Akt and p53 upstream is inhibited by AAE and p53 activated by non-activated pMDM2, p53 inhibitor. Thereby, activated p53 is transcript to p21 and activated p21 protein is combined with Cyclin E-pCDK2 complex. Therefore, we confirmed that AAE-induced cell cycle arrest was occurred by p21-Cyclin E-pCDK2 complex by inhibition of pAkt signal. Because of this cell cycle can't pass to S phase from G1 phase.

Keywords

References

  1. Montalto, G., M. Cervello, L. Giannitrapani, F. Dantona, A. Terranova, and L. A. M. Castagnetta (2002) Epidemiology, risk factors, and natural history of hepatocellular carcinoma. Ann. NY Acad. Sci. 963: 13-20.
  2. Yun, H. J., S. G. Hwang, H. J. Yun, C. H. Kim, G. S. Seo, W. H. Park, and S. D. Park (2006) Anticancer effect of Rheum Rhizoma on human liver cancer HepG2 cells. Korea J. Herbol. 4: 27-36.
  3. Ryu, J. H., S. J. Lee, M. J. Kim, J. H. Shin, S. K. Kang, K. M. Cho, and A. J. Sung (2011) Antioxidant and anticancer activities of Artemisia annua L. and determination of functional compounds. J. Korea Soc. Food Sci. Nutr. 40: 509-516. https://doi.org/10.3746/jkfn.2011.40.4.509
  4. Romero, M. R., M. A. Serrano, M .Vallejo, T. Efferth, M. Alvarez, and J. J. Marin (2006) Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV). Planta Med. 72: 1169-1174. https://doi.org/10.1055/s-2006-947198
  5. Schmid, G., and W. Hofheinz (1983) Total synthesis of qinghaosu. J. Am. Chem. Soc. 105: 624-625. https://doi.org/10.1021/ja00341a054
  6. Avery, M. A., W. K. M. Chong, and White C. Jennings (1992) Stereoselective total synthesis of (dextro)-artemisinin, the antimalarial constituent of Artemisia annua L. J. Am. Chem. Soc. 114: 974-979. https://doi.org/10.1021/ja00029a028
  7. Park, M. T. and S. J. Lee (2003) Cell cycle and cancer. J. Biochem. Mol. Biol. 36: 60-65. https://doi.org/10.5483/BMBRep.2003.36.1.060
  8. Novak, B., J. C. Sible, and J. J. Tyson (2003) Checkpoints in the Cell Cycle, Encyclopedia of Life Sciences. DOI:10.1038/npg.els.0 001355
  9. Lee, M. H. and H. Y. Yang (2003) Regulators of G1 cyclin-dependent kinases and cancers. Cancer Metastasis Rev. 22: 435-449. https://doi.org/10.1023/A:1023785332315
  10. Kang, M. S., S. M. Ju, B. J. Jeon, H. M. Yang, W. S. Kim, and B. H. Jeon (2011) Nardostachys chinensis induces G0/G1 phase cell cycle arrest in U937 cells. Korean J. Oriental Physiol. Pathol. 25: 189-194.
  11. Giono, L. E. and J. J. Manfredi (2007) Mdm2 is required for inhibition of Cdk2 activity by p21, thereby contributing to p53-dependent cell cycle arrest. Mol. Celluar Biol. 11: 4166-4178.
  12. Yoko, O., K. Shohei, O. Toshiyuki, I. Yuko, S. Toshiaki, T. Keiji, M. Norihisa, and G. Yukiko (2002) Akt enhances Mdm2-mediated Ubiquitination and Degradation of p53. J. Biol. Chem. 24: 21843- 21850.
  13. Arnold, J. (1997) p53, the cellular gatekeeper for growth and division. Cell 88: 323-331. https://doi.org/10.1016/S0092-8674(00)81871-1
  14. Moll, U. M. and O. Petrenko (2003) The MDM2-p53 Interaction. Mol. Cancer Res. 1: 1001-1008.
  15. Lee, J. H., W. Y. Choi 1, Y. H. Choi 2 and B. T. Choi (2009) Cell cycle arrest by treatment of D-Ala2-Leu5-enkephalin in human Leukemia cancer U937 cell. J. Life Sci. 5: 620-624.
  16. Arnold, J., F. Zhaohui, W. M. Tak (2014) Coordination and communication between the p53 IGF-1-Akt-TOR signal transduction pathways. Cold Spring Harbor Laboratory Press, 9.
  17. Mirela, F., S. Chintda, E. Carol, and D. Oliver (2012) Effect of OI 3K/Akt pathway inhibition-mediated G1 arrest on chemosensitization in ovarian cancer cells. Anticancer Res. 32: 445-452.
  18. Donnellan, R. and R. Chetty (1999) Cyclin E in human cancers. FASEB J. 13: 773-780. https://doi.org/10.1096/fasebj.13.8.773
  19. Pan, M. H., W. H. Chen, S. Y. Shiau, C. T. Ho, and J. K. Lin (2002) Tangeretin induces cell-cycle G1 arrest through inhibiting cyclindependent kinases 2 and 4 activities as well as elevating Cdk inhibitors p21 and p27 in human colorectal carcinoma cells. Carcinogenesis 10: 1677-1684.

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