DOI QR코드

DOI QR Code

Effect of various additives on reduction of unpleasant odor and inhibition of Bacillus cereus growth in cheonggukjang

다양한 첨가제에 의한 청국장 불쾌취 및 Bacillus cereus 증식의 억제

  • Jeong, Su-Hyeon (Department of Food Science and Technology, Chonbuk National University) ;
  • Park, Song-Yie (National Institute of Crop Science) ;
  • Jeong, Eun-Seon (Jeonbuk Institute for Food-Bioindustry) ;
  • Kim, Yong-Suk (Department of Food Science and Technology, Chonbuk National University) ;
  • Mun, Sung Phil (Department of Wood Science and Technology, Chonbuk National University)
  • Received : 2016.06.27
  • Accepted : 2016.08.09
  • Published : 2016.12.31

Abstract

The effectiveness of various additives in reduction of an unpleasant odor and inhibition of Bacillus cereus growth in Cheonggukjang (CKJ) was investigated. Sensory evaluations of unpleasant odor intensity and taste preference for CKJ were conducted with a 5-point scale. Raw CKJ was rated to have the highest unpleasant odor intensity, followed by CKJ-salt, CKJ-wood vinegar salt, CKJ-red pepper seed oil, and CKJ-bamboo salt in the given order. The test panel had a greater preference for CKJ-red pepper seed oil than for the other CKJ products. The addition of bamboo salt to CKJ was the most effective in inhibition of B. cereus growth in comparison to the other three additives. Volatile compounds in CKJ and CKJ-bamboo salt were identified by GC/MS analysis. 1-Ethoxy-1-methoxy-ethane was responsible for the pleasant odor and its level significantly increased in CKJ-bamboo salt. Consequently, adding bamboo salt to CKJ not only masked and reduced the unpleasant odor, but also inhibited B. cereus growth in CKJ.

청국장의 불쾌취를 줄이고 B. cereus 증식을 억제하는데 다양한 첨가제의 효과를 검토하였다. 관능검사는 불쾌취와 맛에 대하여 각각 5점 척도법으로 실시하였다. 무첨가 청국장의 불쾌취 강도가 가장 높다고 평가되었으며, 소금 첨가구, 목초액 소금 첨가구, 고추씨기름 첨가구, 죽염 첨가구 순으로 높게 나타났다. 맛의 선호도는 고추씨기름 첨가 청국장이 다른 첨가구들보다 높았다. 각 첨가제들의 B. cereus 증식 억제 효과를 확인한 결과, 죽염이 다른 3개 첨가제에 비하여 그 효과가 가장 뛰어났다. 관능검사 결과 및 B. cereus 증식 억제 효과가 좋게 나타난 죽염 첨가 청국장과 무첨가 청국장의 냄새 성분을 추출, 농축하여 GC/MS로 분석하였다. 죽염 첨가 청국장에서 오렌지 유사 향인 1-ethoxy-1-methoxy-ethane이 무첨가구에 비해 크게 증가하였다. 결과적으로 죽염을 청국장에 첨가하는 것은 차폐에 의한 청국장의 불쾌취 감소뿐만 아니라 B. cereus 증식 억제에 효과적임을 확인하였다.

Keywords

References

  1. Kim KJ, Ryu MK, Kim SS. Chungkook-jang Koji fermentation with rice straw. Korean J. Food Sci. Technol. 14: 301-308 (1982)
  2. Heo S, Lee SK, Joo HK. Isolation and identification of fibrinolytic bacteria from Korean traditional chungkookjang. Agr. Chem. Biotechnol. 41: 119-124 (1998)
  3. Kwon HY, Kim YS, Kwon GS, Kwon CS, Sohn HY. Isolation of immuno-stimulating strain Bacillus pumilus JB-1 from chungkook-jang and fermentational characteristics of JB-1. Korean J. Microbiol. Biotechnol. 32: 291-296 (2004)
  4. Choi SH, Ji YA. Changes in flavor of chungkookjang during fermentation. Korean J. Food Sci. Technol. 21: 229-234 (1989)
  5. Bock JY. Changes in chemical composition of steamed soybean during fermentation and in alkylpyrazines during aging of chungkookjang. PhD thesis, Chungang University, Seoul, Korea (1993)
  6. Kim JH. Changes of flavor compounds during chung-kook-jang fermentation. MS Thesis, Konkuk University, Seoul, Korea (1996)
  7. Park WJ, Park HY, Yoo JH, Rhee MS. Effect of Artmisia asiatica Nakai extract on the flavor of chung-kuk-jang. Food Eng. Prog. 5: 115-124 (2011)
  8. Joo HK. Studies on chemical composition of commercial chungkuk-jang and flavor compounds of chung-kuk-jang by mugwort (Artmisia asiatica) or red pepper seed oil. Korea Soybean Digest. 13: 44-56 (1996)
  9. Andersson A, Ronner U, Granum PE. What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens. Int. J. Food Microbiol. 28: 145-155 (1995) https://doi.org/10.1016/0168-1605(95)00053-4
  10. Moon JH, Shin HA, Rha YA, Om AS. The intrinsic antimicrobial activity of bamboo salt against Salmonella enteritidis. Mol. Cell. Toxicol. 5: 323-327 (2009)
  11. Park SJ, Park KY, Jun HK. Effects of commercial salts on the growth of kimchi-related microorganisms. J. Korean Soc. Food Sci. Nutr. 30: 806-813 (2001)
  12. Hwang KM, Oh SH, Park KY. Increased antimutagenic and in vitro anticancer effects by adding green tea extract and bamboo salt during Doenjang fermentation. J. Korean Soc. Food Sci. Nutr. 36: 1-7 (2007) https://doi.org/10.3746/jkfn.2007.36.1.001
  13. Lee KI, Park KY, Ahn HK. The anticancer effects of doenjang made with various kinds of salt. Korean J. Culin. Res. 17: 241-252 (2011)
  14. Park SS. The quality characteristics and taste compounds of fermented fish, jari-jeot with bamboo salt. J. Korean Soc. Food Sci. Nutr. 41: 666-673 (2012) https://doi.org/10.3746/jkfn.2012.41.5.666
  15. Ikegami F, Sekine T, Fujii Y. Anti-dermaptophyte activity of phenolic compounds in "Mokusaku-Eki". Yakugaku Zasshi. 118: 27-30 (1998) https://doi.org/10.1248/yakushi1947.118.1_27
  16. Kang JS, Kim SH, Kim PG, Lee DW, Ryu SP. Differences of wood vinegar ingestion and exercise training on blood lipids, MDA, and SOD activities in rats. J. Life Sci. 19: 1190-1199 (2009) https://doi.org/10.5352/JLS.2009.19.9.1190
  17. Lee SS, Ahn BJ, Cho ST. Antimicrobial activities of wood vinegar and application as natural fungicides and food preservatives. J. Korean Wood Sci. Technol. 38: 341-348 (2010) https://doi.org/10.5658/WOOD.2010.38.4.341
  18. Shon WS, Yoo YC, Kim CY. The effect of NaCl and bamboo salt on the growth of various oral bacteria. J. Korean Acad. Oral Health. 15: 255-268 (1991)
  19. Lee NY, Kim YS, Shin DH. Growth inhibitory effects of chloride salts and organic acid salts against foodborne microorganisms. J. Korean Soc. Food Sci. Nutr. 32: 1233-1238 (2003) https://doi.org/10.3746/jkfn.2003.32.8.1233
  20. Lee KM, Jeong GT, Park DH. Study of antimicrobial and DPPH radical scavenger activity of wood vinegar. Korean J. Biotechnol. Bioeng. 19: 381-384 (2004)
  21. Kim JS, Park SW, Ham YS, Jung SK, Lee SH, Chung SK. Antimicrobial activities and phenolic compounds of pyroligneous liquor. Korean J. Food Preserv. 12: 470-475 (2005)
  22. Nahon DF, Navarro Y Koren PA, Roozen JP, Posthumus MA. Flavor release from mixtures of sodium cyclamate, sucrose, and an orange aroma. J. Agr. Food Chem. 46: 4963-4968 (1998) https://doi.org/10.1021/jf980679e
  23. Zhao J, Dai X, Liu X, Zhang H, Tang J, Chen W. Comparison of aroma compounds in naturally fermented and inoculated Chinese soybean pastes by GC-MS and GC-Olfactometry analysis. Food Control. 22: 1008-1013 (2011) https://doi.org/10.1016/j.foodcont.2010.11.023
  24. Shukla S, Choi TB, Park HK, Kim MH, Lee IK, Kim JK. Determination of non-volatile and volatile organic acids in Korean traditional fermented soybean paste (doenjang). Food Chem. Toxicol. 48: 2005-2010 (2010) https://doi.org/10.1016/j.fct.2010.04.034
  25. Boelens MH. Chemical and sensory evaluation of lavandula oils. Perfum. Flavor. 20: 23-51 (1995)
  26. Ho CT, Carlin JT. Formation and aroma characteristics of heterocyclic compounds in foods. pp. 92-104. In: Flavor Chemistry Trends and Developments. Teranishi R, Buttery RG, Shahidi F (eds). American Chemical Society, Washington, DC, USA (1989)
  27. Bernstein LR, Tanner T, Godfrey C, Noll B. Chemistry and pharmacokinetics of gallium maltolate, a compound with high oral gallium bioavailability. Metal-Based Drugs. 7: 33-47 (2000) https://doi.org/10.1155/MBD.2000.33
  28. Lasekan O. Volatile constituents of roasted tigernut oil (Cyperus esculentus L.). J. Sci. Food Agr. 93: 1055-1061 (2013) https://doi.org/10.1002/jsfa.5846