The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Torilis japonica Extract on DNCB-induced Contact Dermatitis in BALB/c Mice

사상자 추출물이 DNCB로 유도된 BALB/c 마우스의 접촉성 피부염에 미치는 효과

  • Lee, Kyou-Young (Dept. of Oriental Opthalmology and Otolaryngology and Dermatology, College of Korean Medicine, Sangji University) ;
  • Song, Eun-Hye (Dept. of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Hong, Chul-Hee (Dept. of Oriental Opthalmology and Otolaryngology and Dermatology, College of Korean Medicine, Sangji University)
  • 이규영 (상지대학교 한의과대학 안이비인후피부과학교실) ;
  • 송은혜 (상지대학교 한의과대학 약리학교실) ;
  • 홍철희 (상지대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2016.10.06
  • Accepted : 2016.11.10
  • Published : 2016.11.25
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Abstract

목적: 본 연구에서는 TJ 열수추출물이 아토피 피부염에 미치는 효과를 확인하고자 하였다. 방법: 2, 4-dinitrochlorobenzen (DNCB)으로 BALB/c 마우스에 1차 감작 후 3주간 2차 감작을 시행하여 아토피 유사 피부병변을 나타내는 접촉성 피부염을 유발한 뒤 동결건조 처리된 TJ 분말을 (PBS/EtOH/Cremophor=6:1:3)에 용해시켜 쥐의 등 피부에 10, 50 mg/ml 농도로 3주간 도포하였다. 결과: TJ 열수추출물은 아토피 피부 병변의 염증세포 침윤을 억제하였고 (10, 50 mg/ml ) 표피와 진피 두께를 회복시켰으며 (10, 50 mg/ml ), 혈청에서 히스타민 방출을 억제하였다 (00 mg/ml ). 또한 Th2 세포와 관련된 cytokine인 interleukin (IL)-4, IL-5, IL-13과 thymic stromal lymphopoietin (TSLP)의 mRNA 발현 양을 감소시켰고 (10, 50 mg/ml ), Th2 chemokine인 thymus- and activation-regulated chemokine (TARC or CCL17)의 mRNA의 발현 양을 감소시켰다 (10, 50 mg/ml ). 결론: TJ 열수추출물을 BALB/c 마우스에 외용하였을 때 항아토피 효과가 있음을 확인하였으며 따라서 TJ가 아토피 피부염 치료의 외용제로 활용될 수 있을 것으로 사료된다.

Keywords

References

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