Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Citri Reticulatae Viride Pericarpium on 4-Hydroxynonenal-Induced Inflammation in PC12 Cells

청피의 항염증효과

  • Ye, Young Jun (Department of Anatomy-Pointology, College of Korean Medicine, Gachon University) ;
  • Kim, Youn Sub (Department of Anatomy-Pointology, College of Korean Medicine, Gachon University) ;
  • Kang, Mi Suk (Department of Acupuncture and Moxibustion, College of Korean Medicine, Gachon University)
  • 예영준 (가천대학교 한의과대학 해부경혈학교실) ;
  • 김연섭 (가천대학교 한의과대학 해부경혈학교실) ;
  • 강미숙 (가천대학교 한의과대학 침구의학교실)
  • Received : 2016.10.07
  • Accepted : 2016.12.13
  • Published : 2016.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The purpose of this study was to observe the effects of Citri Reticulatae Viride Pericarpium (CP) on 4-Hydroxynonenal (4-HNE)-induced inflammation in PC12 cells. Methods: 4-HNE was treated in PC12 cell to cause inflammatory response, and then treated with CP water extract at 25, 50, and $100{\mu}g/ml$. The phosphorylation of Jun N-terminal kinase (JNK) and the expression of $NF-{\kappa}B$ in PC12 cells were determined by Western blot, respectively. Results: The phosphorylation of JNK was significantly decreased in 4-HNE-stimulated PC12 cell by the treatment of CP extract at $25{\mu}g/ml$. The 4-HNE-induced expression of nuclear factor kappa-light-chain-enhancer of activated B cells ($NF-{\kappa}B$) p65 in nuclear of the cells was significantly decreased in PC12 cell by treatment with CP extract at 25, 50, and $100{\mu}g/ml$. Conclusions: These results suggest that CP water extract has an anti-inflammatory activity through suppressing the JNK and $NF-{\kappa}B$ activation.

Keywords

References

  1. Whitney NP, Eidem TM, Peng H, Huang Y, Zheng JC. Inflammation mediates varying effects in neurogenesis: relevance to the pathogenesis of brain injury and neurodegenerative disorders. J Neurochem. 2009 ; 108(6) : 1343-59. https://doi.org/10.1111/j.1471-4159.2009.05886.x
  2. Kannappan R, Gupta SC, Kim JH, Reuter S, Aggarwal BB. Neuroprotection by spice-derived nutraceuticals: you are what you eat! Mol Neurobiol. 2011 ; 44(2) : 142-59. https://doi.org/10.1007/s12035-011-8168-2
  3. Siddiqui MA, Kumar V, Kashyap MP, Agarwal M, Singh AK, Khanna VK, et al. Short-term exposure of 4-hydroxynonenal induces mitochondria-mediated apoptosis in PC12 cells. Hum Exp Toxicol. 2012 ; 31(4) : 336-45. https://doi.org/10.1177/0960327111432500
  4. Bruckner SR, Estus S. JNK3 contributes to c-jun induction and apoptosis in 4-hydroxynonenal-treated sympathetic neurons. J Neurosci Res. 2002 ; 70(5) : 665-70. https://doi.org/10.1002/jnr.10437
  5. Yin G, Wang Y, Cen XM, Yang M, Liang Y, Xie QB. Lipid peroxidation-mediated inflammation promotes cell apoptosis through activation of NF-${\kappa}B$ pathway in rheumatoid arthritis synovial cells. Mediators Inflamm. 2015 : 460310.
  6. Mattson MP. Roles of the lipid peroxidation product 4-hydroxynonenal in obesity, the metabolic syndrome, and associated vascular and neurodegenerative disorders. Exp Gerontol. 2009 ; 44(10) : 625-33. https://doi.org/10.1016/j.exger.2009.07.003
  7. Purkayastha S, Cai D. Disruption of neurogenesis by hypothalamic inflammation in obesity or aging. Rev Endocr Metab Disord. 2013 ; 14(4) : 351-6. https://doi.org/10.1007/s11154-013-9279-z
  8. Ham MR. Role of macrophage glucose-6-phosphate dehydrogenase in proinflammatory responses and oxidative stress associated with obesity. Doctor thesis. Seoul: Seoul University. 2013.
  9. Cho WJ. Effects of combined and walking exercises on inflammatory markers in obese middle-aged women. Korea J Sport. 2013 ; 11(1) : 251-63.
  10. Oriental University Edit Committee. Herbology. Seoul : YoungLim Co. 2011 : 217.
  11. Baik SO, Bock JY, Chun HJ, Jeong SI, Baek SH, Oh HB, et al. Analysis and quantitative distribution of glycosided flavonoids in citruses and Korean Chung-pi. Anal Sci Technol. 2001 ; 14(4) : 340-8.
  12. Min SS, Yuan TS, Po JC. Antioxidant activities of citrus herbal product extracts. Food Chem. 2008 ; 111 : 892-6. https://doi.org/10.1016/j.foodchem.2008.05.002
  13. Li X, Huang Y, Chen D. Protective effect against hydroxylinduced DNA damage and antioxidant activity of citri reticulatae pericarpium. Adv Pharm Bull. 2013 ; 3(1) : 175-81.
  14. Rho YH, Jeong HW, Lee WS. Experimental study of fraction of Citri Reticulatae Viride Pericarpium extract on the brain damage in cerebral ischemia. Korean J Orient Physiol Pathol. 2002 ; 16 : 893-8.
  15. Kim DK, Lee KT, Eun JS, Zee OP, Lim JP, Eum SS, et al. Anti-allergic components from the peels of Citrus unshiu. Arch Pharm Res. 1999 ; 22(6) : 642-5. https://doi.org/10.1007/BF02975340
  16. Lee HJ. Effects of citro reticulatae viride pericarpium on the vascular endothelial growth factor (VEGF) in the bronchial asthma mouse model. J Pediatr Korean Med. 2006 ; 4 : 181-94.
  17. Jeong HY, Oh CH, Eun JS. Effect of Citri Reticulatae Viride Pericarpium water extract on immunocytes in mice. Korean J Orient Med Pathol. 1998 ; 12 : 55-62.
  18. Jung SY. Anti-obesity effect of Citrus unshiu peel in rats fed a high-fat diet. Master thesis. Seoul: Kyunghee University. 2013.
  19. Jo HK, Han MH, Hong SH, Choi YH, Park C. Ethanol extracts of citrus peel inhibits adipogenesis through AMPK signaling pathway in 3T3-L1 preadipocytes. J Life Sci. 2015 ; 25(3) :285-92. https://doi.org/10.5352/JLS.2015.25.3.285
  20. Choi ES. Effect of citri unshiu pericarpium and citri unshiu pericarpium immaturus on differentiation of pre-adipocyte. Master thesis. Iksan: Wonkwang University. 2014.
  21. Joo KG. Effects of Citri Reticulatae Viride Pericarpium on apoptosis in PC-12 cell. Master thesis. Seongnam: Gachon University. 2016.
  22. Xie QW, Kashiwabara Y, Nathan C. Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase. J Biol Chem. 1994 ; 269(7) : 4705-8.
  23. Fu J, Shi Q, Song X, Xia X, Su C, Liu Z, et al. Tetrachlorobenzoquinone exhibits neurotoxicity by inducing inflammatory responses through ROS-mediated $IKK/I{\kappa}B/NF-{\kappa}B$ signaling. Environ Toxicol Pharmacol. 2016 ; 6 : 241-50.
  24. Yoon JH, Youn K, Ho CT, Karwe MV, Jeong WS, Jun M. P coumaric acid and ursolic acid from corni fructus attenuated ${\beta}$ amyloid25-35-induced toxicity through regulation of the NF-${\kappa}B$ signaling pathway in PC12 cells. J Agric Food Chem. 2014 ; 62(21) : 4911-6. https://doi.org/10.1021/jf501314g
  25. Kang SY, Jung HW, Lee MY, Lee HW, Chae SW, Park YK. Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia. Chin J Nat Med. 2014 ; 12(8) : 573-81. https://doi.org/10.1016/S1875-5364(14)60088-1
  26. Caivano M. Role of MAP kinase cascades in inducing arginine transporters and nitric oxide synthetase in RAW264 macrophages. FEBS Lett. 1998 ; 429(3) : 249-53. https://doi.org/10.1016/S0014-5793(98)00578-X
  27. Nolan GP, Ghosh S, Liou HC, Tempst P, Baltimore D. DNA binding and $I{\kappa}B$ inhibition of the cloned p65 subunit of NF-${\kappa}B$, a rel-related polypeptide. Cell. 1991 ; 64(5) : 961-9. https://doi.org/10.1016/0092-8674(91)90320-X

Cited by

  1. Lipopolysaccharide를 처리한 RAW 264.7 세포에서 백두옹 메탄올 추출물의 항염증 효과 vol.17, pp.1, 2016, https://doi.org/10.15429/jkomor.2017.17.1.1
  2. Can Medicinal Plants and Bioactive Compounds Combat Lipid Peroxidation Product 4-HNE-Induced Deleterious Effects? vol.10, pp.1, 2016, https://doi.org/10.3390/biom10010146
  3. 최근 10년간 한방비만학회지의 연구동향 분석: 2010-2019년 한방비만학회지 게재논문을 중심으로 vol.20, pp.2, 2016, https://doi.org/10.15429/jkomor.2020.20.2.149
  4. 청피 물 추출물의 항염증 효과와 기전 연구 vol.34, pp.1, 2016, https://doi.org/10.15204/jkobgy.2021.34.1.034
  5. Antioxidant Activities and Quality Characteristics of Sponge Cake Added with Premature Mandarin Peel Powder vol.50, pp.9, 2021, https://doi.org/10.3746/jkfn.2021.50.9.981