Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Effects of Direct Moxibustion Applied to EX-LE4 and EX-LE5 on the Pain Behavior and Expression of TRPM8 in the Rat Model of Ambient Cold Exposed Osteoarthritis

추위에 노출된 슬관절염 모델에서 내슬안, 외슬안 직접구가 통증행동과 TRPM8 발현에 미치는 영향

  • Ji, Byeong Uk (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Kim, Yiquot (Department of Korean Medical Science, School of Korean Medicine, Pusan National University) ;
  • Lee, Ji Eun (Healthy Aging Korean Medical Research Center, Pusan National University) ;
  • Koo, Sungtae (Department of Korean Medical Science, School of Korean Medicine, Pusan National University)
  • 지병욱 (부산대학교 한의학전문대학원 한의과학과) ;
  • 김이꽃 (부산대학교 한의학전문대학원 한의과학과) ;
  • 이지은 (부산대학교 건강노화 한의과학 연구센터) ;
  • 구성태 (부산대학교 한의학전문대학원 한의과학과)
  • Received : 2016.11.24
  • Accepted : 2016.12.13
  • Published : 2016.12.27
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Abstract

Objectives : The aim of the study is to investigate the effects of moxibustion on the pain behavior and expression of TRPM8 in the dorsal root ganglion(DRG) in the rat model of ambient cold(AC) exposed osteoarthritis(OA). Methods : OA was induced by the injection of $50{\mu}l$ of 2% monosodium iodoacetate(MIA) into the knee joint cavity. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. For the AC exposure, the animals were housed in 6 h/day at $4^{\circ}C$ for 14 days after MIA injection. Moxibustion treatment was performed at EX-LE4 and EX-LE5 with 5 cons(1, 7 or 10 mg) per day for 13 days from 5 days after MIA injection. The expressions of TRPM8 in DRG were measured by western blotting analysis. Results : The WBFs of MIA-AC group were decreased significantly compared to MIA group at 2, 3, 6, 7, 8 and 9 days after arthritis induction. After the first 6 h-AC exposure, expressions of TRPM8 in MIA-AC group were increased significantly compared to those of naive group. After moxibustion treatment, only the WBFs of 7 mg treated group were restored significantly. Moreover, the over-expressions of TRPM8 were attenuated by the moxibustion treatment in AC exposed rats. Conclusions : The data suggest that AC can increase arthritic knee pain via up-regulated TRPM8 and moxibustion treatment improve the arthritic pain via modulation of TRPM8 expression in DRG in the rat model of AC exposed MIA induced arthritis.

Keywords

References

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