Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Profiling of flavonoid glycosides in fruits and leaves of jujube (Zizyphus jujuba var. inermis (Bunge) Rehder) using UPLC-DAD-QTOF/MS

UPLC-DAD-QTOF/MS를 이용한 대추나무(Zizyphus jujuba var. inermis (Bunge) Rehder) 잎과 열매의 플라보노이드 배당체 분석

  • Lee, Min-Ki (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Heon-Woong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Young Jin (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Seon-Hye (National Academy of Agricultural Science, Rural Development Administration) ;
  • Jang, Hwan-Hee (National Academy of Agricultural Science, Rural Development Administration) ;
  • Jung, Hyun-Ah (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Kim, Sook-Bae (Department of Food Science and Human Nutrition, Chonbuk National University) ;
  • Lee, Sung-Hyen (National Academy of Agricultural Science, Rural Development Administration) ;
  • Choe, Jeong-Sook (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jung-Bong (National Academy of Agricultural Science, Rural Development Administration)
  • 이민기 (국립농업과학원 농식품자원부) ;
  • 김헌웅 (국립농업과학원 농식품자원부) ;
  • 김영진 (국립농업과학원 농식품자원부) ;
  • 이선혜 (국립농업과학원 농식품자원부) ;
  • 장환희 (국립농업과학원 농식품자원부) ;
  • 정현아 (전북대학교 식품영양학과) ;
  • 김숙배 (전북대학교 식품영양학과) ;
  • 이성현 (국립농업과학원 농식품자원부) ;
  • 최정숙 (국립농업과학원 농식품자원부) ;
  • 김정봉 (국립농업과학원 농식품자원부)
  • Received : 2016.09.07
  • Accepted : 2016.11.11
  • Published : 2016.12.30
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Abstract

Flavonoids, non-nutrient secondary metabolites of plants, are widely distributed in commonly consumed agro-food resources. Flavonoids include aglycones, and their glycosides are reported to have potential health-promoting compounds. The aim of this study was to investigate flavonoid glycosides in the fruit and leaves of Zizyphus jujuba var. inermis (Bunge) Rehder (jujube). A total of six flavonoids (five flavonols and one chalcone) were identified in jujube fruit and leaves by using ultra-performance liquid chromatography-diode array detector-quadrupole time of flight mass spectrometry along with chemical library and an internal standard. In positive ion mode, six flavonoids were linked to the C- and O-glycosides which were conjugated with sugar moieties based on kaempferol, quercetin, and phloretin aglycones. Total flavonoid contents of leaves (8,356.5 mg/100 g dry weight (DW)) was approximately 900-fold higher than that of fruit (fresh fruit, 13.6 mg/100 g dry DW; sun-dried fruits, 9.2 mg/100 g dry DW). Quercetin 3-O-rutinoside (rutin) and quercetin 3-O-robinobioside were the predominant flavonols in fruit and leaves of jujube. In particular, rutin had the highest content (6,735.2 mg/100 g DW) in leaves, and rutin is a widely reported bioactive compound. Phloretin 3',5'-di-C-glucoside (chalcone type) was detected only in leaves. The leaves of jujube contain a high content of flavonoids and the results of this study indicate that jujube leaves may be a source of bioactive flavonoids.

본 연구에서는 국내산 대추나무의 잎과 열매의 flavonoid 배당체를 조사하기 위하여 선행 연구된 인용문헌을 바탕으로 대추나무, 묏대추나무, 야생대추나무 등 대추나무속(Zizyphus)에 따른 flavonoid의 화학적 정보 수집 및 정리하여 대추나무속의 flavonoid 라이브러리를 제작하였다. 각 flavonoid 개별성분은 UPLC-DAD-QTOF-ESI/MS를 사용하여 분석하였으며 제작된 라이브러리의 정보를 이용하여 국내산 대추나무로부터 총 6종의 flavonoid를 확인하였다. 이를 통하여 국내산 대추나무 잎의 주요 성분은 quercetin 배당체인 rutin임을 확인하였고, 특히 quercetin 3-O-robinobioside 및 isoquercitrin는 구축된 라이브러리를 바탕으로 대추나무 잎에서는 처음 확인된 것을 알 수 있었다. 본 연구와 같이 대추나무속 flavonoid의 화합물 정보를 포함하여 제작한 라이브러리는 선행된 연구와의 차이점 판단을 가능하게 할 것으로 사료된다.

Keywords

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