Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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MALDI TOF MS for the identification of Salmonella spp. from swine

돼지유래 Salmonella속 균의 동정을 위한 MALDI TOF MS 활용

  • Sohn, Jun Hyung (North Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Jeon, Woo Jin (North Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Lee, Young Mi (North Branch, Gyeongbuk Veterinary Service Laboratory) ;
  • Kim, Seon Soo (North Branch, Gyeongbuk Veterinary Service Laboratory)
  • 손준형 (경북동물위생시험소 북부지소) ;
  • 전우진 (경북동물위생시험소 북부지소) ;
  • 이영미 (경북동물위생시험소 북부지소) ;
  • 김선수 (경북동물위생시험소 북부지소)
  • Received : 2016.11.28
  • Accepted : 2016.12.26
  • Published : 2016.12.30
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Abstract

Salmonella is one of the most common bacteria that causes heavy losses in swine industry and major causative pathogen of food poisoning in public health. Various methods for the identification of Salmonella such as Gram staining, agglutination test, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) have been used. Several studies have demonstrated that Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI TOF) Mass Spectrometry (MS) identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. In this study, MALDI TOF MS could provide rapid, accurate identification of Salmonella spp. from swine compared with end point PCR and real time PCR.

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References

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