Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Nematicidal Activity of Streptomyces flavogriseus KRA15-528 to Meloidogyne incognita

Meloidogyne incognita에 대한 Streptomyces flavogriseus KRA15-528의 살선충활성

  • Oh, Mira (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Han, Jae Woo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Choi, Jung Sup (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Choi, Yong Ho (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Jang, Kyoung Soo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Choi, Gyung Ja (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Kim, Hun (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
  • 오미라 (한국화학연구원 친환경신물질연구센터) ;
  • 한재우 (한국화학연구원 친환경신물질연구센터) ;
  • 최정섭 (한국화학연구원 친환경신물질연구센터) ;
  • 최용호 (한국화학연구원 친환경신물질연구센터) ;
  • 장경수 (한국화학연구원 친환경신물질연구센터) ;
  • 최경자 (한국화학연구원 친환경신물질연구센터) ;
  • 김헌 (한국화학연구원 친환경신물질연구센터)
  • Received : 2016.06.22
  • Accepted : 2016.08.30
  • Published : 2016.12.31
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Abstract

Plant disease caused by root-knot nematode is a major problem in crop production. Using of chemical pesticides, one of the most efficient methods to control nematodes, have raised issues in toxicity to humans and animals and environmental pollution. In this study, to select actinomycete strains that have potential to serve as a microbial agent for control of nematodes, we investigated nematicidal activity of culture broth from 670 Streptomyces isolates. A culture filtrate of KRA15-528 isolate that was identified as S. flavogriseus on the basis of 16S rRNA sequence analysis, showed strong nematicidal activity against second stage of juveniles of Meloidogyne incognita and inhibited egg hatching; exposure to 10% of culture filtrate resulted in 71% juvenile mortality at 48 hours afters treatment and suppressed egg hatching by 54% at 9 days after treatment. When the KRA15-528 culture filtrate was partitioned with ethyl acetate and butanol, ethyl acetate layer exclusively showed strong activity; 91%, 53%, 30% of mortality at 1,000, 500, $250{\mu}g/ml$, respectively. Additionally, the culture filtrate suppressed gall formation on cucumber plant by M. incognita with no phytotoxicity. These results suggest that S. flavogriseus KRA15-528 has potential to serve as a microbial nematicide for the control of root-knot nematode disease.

뿌리혹선충에 의한 병 발생은 작물수확량의 감소를 초래하여 중요한 문제로 인식되고 있다. 화학농약의 사용은 선충방제를 위해 효과적인 방법 중의 하나로 고려되지만 환경 오염이나 인축에 대한 독성과 같은 문제를 야기하고 있다. 본 연구에서는 친환경 살선충제 개발에 사용될 수 있는 미생물을 선발하고자, 토양으로부터 분리한 670여 개의 방선균을 대상으로 뿌리혹선충에 대한 살선충 활성을 조사하였다. 이들 중 가장 우수한 살선충 활성을 나타내는 KRA15-528 균주를 선발하였으며, 16S rRNA 유전자 염기서열에 기초하여 S. flavogriseus로 동정하였다. S. flavogriseus KRA15-28 균주 배양여액을 최종농도 10%가 되도록 J2 단계의 유충과 선충알에 각각 처리 시, 48시간 후 71%의 선충 치사율과 9일째에 54%의 선충 알 부화 억제율을 확인하였다. 배양여액을 에틸아세테이트, 부탄올을 이용하여 용매 분획하여 에틸 아세테이트와 부탄올, 물 추출물에 대한 살선충 활성을 조사한 결과, 에틸 아세테이트 추출물로부터만 높은 살선충 활성을 확인하였다. 1,000, 500, $250{\mu}g/ml$의 농도에서 각각 91%, 53%, 30%의 치사율을 나타내었다. 이외에도, 오이에 대한 뿌리 혹 선충병 방제활성을 조사한 결과, 배양여액 처리구에서 눈에 띄게 뿌리혹형성이 억제되는 현상을 관찰하였다. 따라서, S. flavogriseus KRA15-528은 뿌리혹선충병을 방제에 사용할 수 있는 미생물제제로서의 가능성이 있음을 확인할 수 있었다.

Keywords

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