Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Extracts Derived from Red Ginseng Residue on Antioxidant Activity and Elastase Inhibition

홍삼박추출물의 항산화활성 및 주름개선 효과

  • Lee, Mi-Yeon (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics) ;
  • Kim, Bo-Ae (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics) ;
  • Yang, Jae-Chan (Mokwon University, College of Sciences & Technology, Division of Biomedicinal & Cosmetics)
  • 이미연 (목원대학교 테크노과학대학 생의약화장품학부) ;
  • 김보애 (목원대학교 테크노과학대학 생의약화장품학부) ;
  • 양재찬 (목원대학교 테크노과학대학 생의약화장품학부)
  • Received : 2016.10.17
  • Accepted : 2016.12.09
  • Published : 2016.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

We produced the Red ginseng residue water(RGW), ethanol(RGE), 1,3-butylene glycol(RGB) extract from Red ginseng residues, analyzed the components of the extracts by HPLC, and evaluated the cell viability on B16F10, antioxidant and anti-wrinkle effects for application of cosmetics. As a result, RGW, RGE, RGB have various ginsenoside and its content of RGB were higher than RGW, RGE as component analysis by using high performance liquid chromatography(HPLC). RGW showed similar with RGB in cell viability on B16F10 which were higher than RGE. DPPH radical scavenging activity increased according to the RGE>RGB>RGW. SOD-like activity increased according to the RGB>RGE>RGW. Also, elastase inhibition effect increased according to the RGW>RGB>RGE. These results suggested that RGB and RGW may have potential for the application of antioxidant and anti-wrinkle effects for cosmetics.

본 연구는 홍삼박 물추출물(RGW), 에탄올추출물(RGE), 1,3-B.G추출물(RGB)을 HPLC로 성분 분석과 B16F10에 대한 세포생존율, 항산화능 및 주름개선 효능 평가를 실시하여 홍삼박 추출물의 화장품 소재로서의 응용가능성을 알아보았다. 추출물의 HPLC 성분 분석 결과, 세 가지 추출물 모두 다양한 종류의 진세노사이드가 검출되었으며 그 중 RGB가 가장 많은 함량의 진세노사이드가 검출되었다. B16F10의 세포생존율 측정 결과, RGW와 RGB가 유사하며, RGE보다 높은 생존율을 보여주었다. DPPH radical 소거능 측정 결과 RGE>RGB>RGW 순으로 나타났으며 SOD유사활성능 측정 결과는 RGB>RGE>RGW순으로 활성능을 보여주었다. Elastase 저해능 측정 결과에서는 RGW>RGB>RGE 순으로 활성을 보였다. 위와 같은 결과를 종합하여 볼 때 피부안전성이 우수하며 항산화 및 주름개선 화장품소재로서 RGW와 RGB가 적합할 것으로 판단된다.

Keywords

References

  1. J. W. Lee, J. H. Do, Current studies on browning reaction products and acidic polysaccharide in korean red ginseng, J. Ginseng Res, 30(1), 41(2006). https://doi.org/10.5142/JGR.2006.30.1.041
  2. J. D. Park, Recent studies on the chemical constituents of Korean ginseng (Panax ginseng C.A. Meyer). Korean J. Ginseng Sci, 20(4), 389(1996).
  3. L. i. Gang, W. Zhenhua, S. Yaxuan, L. Ke, W. Ziren, Ginsenoside 20(S)-protopanaxadiol inhibits the proliferation and invasion of human fibrosarcoma HT1080 cell. Basic Clin Pharmacol Toxicol 98(6), 588(2006). https://doi.org/10.1111/j.1742-7843.2006.pto_415.x
  4. J. W. Lee, Sohn Ho, J. H. Do, Function of the water soluble browning reaction products isolated from Korean red ginseng, J. Ginseng Res, 24(1), 35(2000).
  5. H. J. Lee, Y. H. Lee, S. K. Park, et al. Korean red ginseng (Panax ginseng) improves insulin sensitivity and attenuates the development of diabetes in Otsuka Long-Evans Tokushima fattyrats. Metabolism. 58(8), 1170 (2009). https://doi.org/10.1016/j.metabol.2009.03.015
  6. V. Vuksan, M. K. Sung, J. L. Sievenpiper, et al. Korean red ginseng (Panax ginseng) improves glucose and insulin regulation in well-controlled, type 2 diabetes: results of a randomized, double-blind, placebocontrolled study of efficacy and safety, Nutr Metab Cardiovasc Dis, 18(1), 46(2008). https://doi.org/10.1016/j.numecd.2006.04.003
  7. H. A. Kim, I. G. Oh, K. H. Park, N. M. Kim, J. H. Do, et al, Stimulatory effect of dietary red ginseng on epidermal hydration and ceramide levels in ultraviolet-irradiated hairless mice, J. Med Food, 12(4), 746(2009) https://doi.org/10.1089/jmf.2008.1185
  8. A. B Fishbein, C. Z. Wang, X. L. Li et al, Asian ginseng enhances the anti-proliferative effect of 5-fluorouracil on human colorectal cancer: comparison between white and red ginseng, Arch Pharm Res, 32(4), 505(2009). https://doi.org/10.1007/s12272-009-1405-9
  9. S. Y. Hwang, D. J. Son, I. W. Kim et al, Korean red ginseng attenuates hypercholesterolemia enhanced platelet aggregation through suppression of diacylglycerol liberation in high cholesterol-diet-fed rabbits, Phytother Res, 22(6), 778(2008). https://doi.org/10.1002/ptr.2363
  10. S. Y. Yoon, J. Y. Park, J. B. Lee et al, Ginsenoside Rg3 regulates S-nitrosylation of the NLRP3 inflammasome via suppression of iNOS, Biochemical and biophysical Res, 463(4), 1184(2015). https://doi.org/10.1016/j.bbrc.2015.06.080
  11. S.B. Curri et al, "Dermocosmetic Activity of Ginsenosides. Note III: Long-Term Evaluation of the Moisturizing and Tonifying Effect on the Face Skin", 217(1986).
  12. J. H. Lee, S. H. Cho, M. Y. Yun, S. k. An, H. H. Jang, S. N. Lee, G. Y. Song, Anti-wrinkle effect of rare ginsenosides, produced from ginsenoside Rd, J. Aesthet Cosmetol, 13(6), 909(2015).
  13. H. S. Sung, S. K. Yoon, W. J. Kim, C. B. Yang, Relationship between chemical components and theirields of red ginseng extract by various extracting conditions. Korean J. Ginseng Res, 9(2), 170(1988).
  14. J. H. Choi, C. M Kim, I. H. Choi, A Study on Improving Egg Freshness Using Red Ginseng Marc Powder to Laying Hens., J. Environmental Science International, 24(9), 1233 (2015). https://doi.org/10.5322/JESI.2015.24.9.1233
  15. C. J. Lee, H. S. Han, C. S. Jhune, J. C. Cheong, J. A et al, Development of new substrate using redginseng marc for bottle culture of oyster mushroom (Pleurotus ostreatus), J. Mushroom science and production, 9(4), 139(2011).
  16. Y. Y. Kim, I. S. Kim, O. J. Park, Y. M. Kim, EGEG induces apoprosis under hypoxic state in B16F10 melanoma cancer cells, J. llife science, 21(2), 250(2010).
  17. W. K. Oh, K. B Kim, J. Y. Lim, K. K. Lee, Y. D. Kwon, S. R. Yeom, Y. S. Song, Effects of dokhwalkisaeng-tang on melanin synthesis inhibition and gene expression in B16f10 malnoma cells, J. pysology & pathology, 23(1), 63(2009).
  18. E. H. Lee, S. Y. Cho, S. J. Kim, E, S, Shin, H, K. Chang, T. R. Lee, Ginsenoside F1 protects human HaCaT keratinocytes from ultraviolet-B-induced apoptosis by maintaining constant levels of Bcl-2. J. invset. Dermatol. 121(3), 607(2003). https://doi.org/10.1046/j.1523-1747.2003.12425.x
  19. J. Chen, H. Peng, Xi. Ou-Yang, X. He, Research on the antitumor effect of ginsenoside Rg3 in B16 melanoma cells. Melanoma Res, 18(5), 322(2008). https://doi.org/10.1097/CMR.0b013e32830b3536
  20. E. Tapani, M. Taavitsainen et al, Toxicity of ethanol in low concentrations. Experimental evaluation in cell culture, Acta radiol, 37(6), 923 1996). https://doi.org/10.1177/02841851960373P296
  21. J. J. Cho, K. K. Lee, B. K. Jo, J. D. Choi, Isolation and characterization of elastase inhibitor from Areca catechu, J. Society of Cosmetic Scientists of Korea. 26(1), 163(2000).
  22. Y. H. Choi, S. E. Kim, J. H, Y. H. Han, M. J. Lee,Antibacterial and antioxidative activity of roasted coffee and red ginseng mixture extracts, J. Korean society of food science and nutrition, 41(3), 320(2012). https://doi.org/10.3746/jkfn.2012.41.3.320
  23. S. Y. Hwang, S. H. Ahn. Biological activities and cell proliferation effects of red ginseng ethanol extracts, J. Phamacopuncture, 55(2011).
  24. J. M. McCord, I. Fridovich, The utility of superoxide dismutase in studying free radical reactions. I. Radicals generated by the interaction of sulfite, dimethyl sulfoxide, and oxygen, J. Biol. Chem, 244(22), 6053(1963).
  25. H. J. Lee, D. Y. Kim, C. C. Chang, Antioxidant effects of korean red ginseng components on the antioxidant enzymes activity and lipid peroxidation in the liver of mouse treated with paraquat, J. Ginseng Res, 23(3), 182(1999).
  26. K..S. Sung, C Chun, Y. H. Kwon, K. H. Kim, C. C. Chang, Effects of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in the liver of mice, J. Ginseng Res, 24(1), 29(2000).
  27. K. S. Kang, I. D. Kim, R. H. Kwon, Y. Y. Heo, S. H. Oh, M. A. Kim, H. J. Jung, H. Y. Kang, B. J. Ha, The evaluation of anti-wrinkle effects in oriental herb extract, J. Life Science, 17(8), 1147(2007). https://doi.org/10.5352/JLS.2007.17.8.1147
  28. R. Voegeli et al, Elastase and tryptase determination on human skin surface. Cosmetic & Toiletries 111, 51(1996).
  29. S. M Lee, Y. H Im, S. C. Kim, J. H. Choi, J. H. Kim, Anti-wrinkle effects of converted protopanaxatriol prepared from Korean Red Ginseng, Kor J Aesthet Cosmetol, 11(5), 877 (2013).

Cited by

  1. 구아바 잎 추출물의 총 폴리페놀과 플라보노이드 함량 및 항 염증 억제 효과 vol.35, pp.1, 2016, https://doi.org/10.12925/jkocs.2018.35.1.254