Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Isolation and Characterization of Plant Growth Promoting Rhizobacteria From Button Mushroom Compost

  • Oh, Sung-Hoon (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Lee, Chang-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2015.11.11
  • Accepted : 2016.03.11
  • Published : 2016.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

An auxin-producing bacteria (strain 5-1) was isolated from button mushroom compost in Boryeong-Si, Chungcheongnam-Do. The 5-1 strain was classified as a novel strain of Enterobacter aerogenes based on chemotaxonomic and phylogenetic analyses. The isolated E. aerogenes 5-1 was confirmed to produce indole-3-acetic acid (IAA), one of the auxin hormones, using TLC and HPLC analyses. When the concentration of IAA was assessed by performing HPLC quantitative analysis, a maximum concentration of IAA of $109.9mgL^{-1}$ was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 24 h at $35^{\circ}C$. Acidification of the culture was deemed caused by an increase of IAA because a negative relationship between IAA production and pH was observed. Supplementation with a known precursor of IAA production, L-tryptophan, appeared to induce maximal production at 0.1% concentration, but it reduced production at concentrations above 0.2%. To investigate the growth-promoting effects to crops, the culture broth of E. aerogenes 5-1 was used to inoculate water cultures and seed pots of mung bean and lettuce. In consequence, adventitious root induction and root growth of mung bean and lettuce were two times higher than those of the control.

Keywords

References

  1. Anroun H, Beauchamp CJ, Goussard N, Chabot R, Lalande R. 1998. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on non-legumes: effect on radishes (Raphanus sativus L.). Plant Soil 204: 57-67. https://doi.org/10.1023/A:1004326910584
  2. Barazani O, Friedman J. 1999. Is IAA the major root growth factor secreted from plant-growth-mediating bacteria. Journal of Chemical Ecology 25:2397-2406. https://doi.org/10.1023/A:1020890311499
  3. Ezaki T, Hashimoto Y, Yabuuchi E. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in micro-dilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. International journal of Systematic Bacteriology 39:224-229. https://doi.org/10.1099/00207713-39-3-224
  4. Gamalero E, Fracchia L, Cavaletto M, Garbaye J, Frey-Klett P, Varese GC, Martinotti MG. 2003. Characterization of functional traits of two fluorescent Pseudomonas isolated from basidiomycetes of ectomycorrhizal fungi. Soil Biology & Biochemistry 35:55-63. https://doi.org/10.1016/S0038-0717(02)00236-5
  5. Joshi KK, Kumer V, Dubey RC, Masheshwari DK, Bajapai VK, Kang SC. 2006. Effect of chemical fertilizer-adaptive variants, Pseudomonas aerugininosa GRC2 and Azotobacter chroococcum ACI, on macrophominia paseolina causing charcoal rot of Brassica juncea. Korean Journal of Environmental Agriculture 25:228-235. https://doi.org/10.5338/KJEA.2006.25.3.228
  6. Jung HK, Kim JR, Woo SM, Kim SC. 2006. An auxin producing plant growth promoting rhizobacterium Bacillus subtilis AH18 which has siderophore-producing biocontrol activity. Korean Journal of Microbiology and Biotechnology 34:94-100.
  7. Jung HK, Kim JR, Woo SM, Kim SD. 2007. Selection of the auxin, siderophore, and cellulase-producing PGPR, Bacillus licheniformis K11 and its plant growth promoting mechanisms. Journal of the Korean Society for Applied Biological Chemistry 50(1):23-28.
  8. Jung YP, Kyung KC, Jang KY, Yoon MH. 2011. Isolation and characterization of plant growth promoting rhizobacteria from waste mushroom bed from Agaricus bisporus. Korean Journal of Soil Science and Fertilizer 44(5): 866-871. https://doi.org/10.7745/KJSSF.2011.44.5.866
  9. Kloepper JW, Leong J, Teintze M, Schroth MN. 1980. Enhancement plant growth by siderophore produced by plant growth-promoting rhizobacteria. Nature 286:885-886. https://doi.org/10.1038/286885a0
  10. Lee CJ, Lee HH, Yoon MH. 2015. Plant growth promotion effect of auxin producing bacteria isolated from soil of Oyster mushroom farmhouse. Journal of Mushrooms 13:1-7. https://doi.org/10.14480/JM.2015.13.1.1
  11. Lim HS, Lee JM, Kim SD. 2002. A plant growth promoting Pseudomonas fluorescens GL20: Mechanism for disease suppression, outer membrane receptor for ferric siderophore, and genetic improvement for increased biocontrol efficacy. Journal of Microbiology and Biotechnology 12:249-257.
  12. Mehnaz S, Mirza MS, Haurat J, Bally R, Normand P, Bano A, Malik KA. 2001. Isolation and 16S rRNA sequence analysis of the beneficial bacterium from the rhizosphere of rice. Canadian Journal of Microbiology 472:110-117.
  13. Ouzari H, Khsairi A, Raddadi N, Hassen A, Zarrouk M, Daffonchio D, Boudabous A. 2008. Diversity of auxin-producing bacteria associated to Pseudomonas savastanoi-induced olive knots. Journal of Basic Microbiology 48(5):370-377. https://doi.org/10.1002/jobm.200800036
  14. Pishchik VN, Vorobyev NI, Chernaeva II, Kozhemyakov AP, Alexeev YV, Lukin SM. 2002. Experimental and mathematical simulation of plant growth promoting rhizobacteria and plant interaction under cadmium stress. Plant Soil 243:173-186. https://doi.org/10.1023/A:1019941525758
  15. Pozem MJ, Aguilar CA, Gaudot CD, Barea JM. 1999. 1,3-$\beta$-Glucanase activities in tomato roots inoculated with arbuscular mycorrhizal fungi and Phytopthora parasitica and their possible involvement in bio protection. Plant Science 141:149-157. https://doi.org/10.1016/S0168-9452(98)00243-X
  16. Ramamoorthy V, Viswanathan R, Raguchander, Prakasam V, Samiyappan R. 2001. Induction of systemic resistance by plant promoting rhizobacteria in crop plants against pests and diseases. Crop Protection 20:1-11. https://doi.org/10.1016/S0261-2194(00)00056-9
  17. Wei G, Kleopper JW, Tuzun S. 1996. Induced systemic resistance to cucumber diseases plant growth by plant promoting rhizobacteria under field conditions. Phytopathology 86:221-224. https://doi.org/10.1094/Phyto-86-221
  18. Zimmer W, Wesche M, Timmermans L. 1998. Identification and isolation of indole-3-pyruvate decarboxylase gene from Azopirillum brasilense, sequencing and functional analysis of gene locus gene. Current Microbiology 36:327-331. https://doi.org/10.1007/s002849900317

Cited by

  1. Mechanisms of herbicide resistance in weeds vol.44, pp.1, 2016, https://doi.org/10.7744/kjoas.20170001
  2. Comparing the composting characteristics of food waste supplemented with various bulking agents vol.46, pp.4, 2016, https://doi.org/10.7744/kjoas.20190072
  3. Production of liquid fertilizer from broken eggs and evaluation of its effect on lettuce growth vol.47, pp.1, 2016, https://doi.org/10.7744/kjoas.20190077
  4. Effect of organic fertilizer mixed with dehydrated food waste powder on growth of leaf lettuce vol.47, pp.4, 2016, https://doi.org/10.7744/kjoas.20200085
  5. A highly efficient auxin-producing bacterial strain and its effect on plant growth vol.19, pp.1, 2021, https://doi.org/10.1186/s43141-021-00252-w
  6. A highly efficient auxin-producing bacterial strain and its effect on plant growth vol.19, pp.1, 2021, https://doi.org/10.1186/s43141-021-00252-w