DOI QR코드

DOI QR Code

Assessment of Antioxidant Activity of Garlic (Allium sativum L.) Peels by Various Extraction Solvents

  • Kim, Gyeong-Hwuii (Department of Biological Science and Technology, Yonsei University) ;
  • Duan, Yishan (Department of Food Science and Technology, Pusan National University) ;
  • Lee, Sang-Chang (College of Nano Science and Technology, Office of Administration, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • Received : 2016.02.17
  • Accepted : 2016.04.01
  • Published : 2016.03.30

Abstract

This study was carried out to investigate the antioxidant activities, total phenol, and flavonoid contents of 70% methanol, 70% ethanol, and chloroform-methanol (CM, 2:1, v/v) extracts from garlic (Allium sativum L.) peels. Antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical and ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] radical scavenging activity, ${\beta}$-carotene bleaching assay, and ferric reducing antioxidant power. Total phenol and flavonoid were in a range of 32.59-63.05 mg CAE/g, 5.26-9.22 mg QE/g, respectively. Total phenol and flavonoid were predominant with the values of $63.05{\pm}0.20mgCAE/g$ and $9.22{\pm}0.17mgQE/g$ in 70% ethanol extract. Additionally, 70% ethanol extract also displayed the strongest antioxidant activity followed by 70% methanol extract and CM extract in all assays. These results showed that garlic peel can be used as natural antioxidant in nutraceutical preparations to prevent human diseases.

Keywords

References

  1. S. K. Banerjee, P. K. Mukherjee and S. K. Maulik, Garlic as an Antioxidant: The Good, the bad and the ugly, Phytother. Res., 17, 97 (2003). https://doi.org/10.1002/ptr.1281
  2. S. P. Hussain, L. N. Jannu and A. R. Rao, Chemopreventive action of garlic on methylcholanthreneinduced carcinogenesis in the uterine cervix of mice, Cancer Letters, 49, 175 (1990). https://doi.org/10.1016/0304-3835(90)90155-Q
  3. S. Oommen, R. J. Anto, G. Srinivas and D. Karunagaran, Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells, European J. Pharmaco., 485, 97 (2004). https://doi.org/10.1016/j.ejphar.2003.11.059
  4. H. Amagase, Clarifying the Real Bioactive Constituents of Garlic, J. Nutr., 136, 716S (2006). https://doi.org/10.1093/jn/136.3.716S
  5. V. Lanzotti, The analysis of onion and garlic, J. Chromatogr. A, 1112, 3 (2006). https://doi.org/10.1016/j.chroma.2005.12.016
  6. M. Ali, M. Thomson, M. Afzal, Garlic and onions: their effect on eicosanoid metabolism and its clinical relevance, Prostag. Leukotr. Essential Fatty Acids, 62, 55 (2000). https://doi.org/10.1054/plef.1999.0124
  7. S. Ankri and D. Mirelman, Antimicrobial properties of allicin from garlic, Microbes Infect., 2, 125 (1999).
  8. M. Ali, K. K. Al-Qattan, F. Al-Enezi, R. M. A. Khanafer and T. Mustafa, Effect of allicin from garlic powder on serum lipids and blood pressure in rats fed with a high cholesterol diet, Prostag. Leukotr. Essential Fatty Acids, 62, 253 (2000). https://doi.org/10.1054/plef.2000.0152
  9. M. Laguerre, J. Lecomte and P. Villeneuve, Evaluation of the ability of antioxidants to counteract lipid oxidation: Existing methods, new trends and challenges, Progress Lipid Res., 46, 244 (2007). https://doi.org/10.1016/j.plipres.2007.05.002
  10. R. H. Liu, Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals, American Soc. Clinical Nutr., 78, 517S (2003). https://doi.org/10.1093/ajcn/78.3.517S
  11. R. Prior and G. H. Cao, Antioxidant phytochemicals in fruits and vegetables: diet and health implications, Hort Sci., 35, 588 (2000).
  12. M. Ichikawa, K. Ryu, J. Yoshida, N. Ide , Y. Kodera ,T. Sasaoka and R. T. Rosen, Identification of six phenylpropanoids from garlic skin as major antioxidants, J. Agric. Food Chem., 51, 7313 (2003). https://doi.org/10.1021/jf034791a
  13. Y. S. Duan, M. A. Kim, J. H. Seong, Y. G. Lee, D. S. Kim, H. S. Chung and H. S. Kim, Impacts of various solvent extracts from wild haw (Crataegus pinnatifida Bunge) pulpy on the antioxidative activities, J. East Asian Soc. Dietary Life, 24, 392 (2014).
  14. A. Wojdylo, J. Oszmianski and R. Czemerys, Antioxidant activity and phenolic compounds in 32 selected herbs, Food Chem., 105, 940 (2007). https://doi.org/10.1016/j.foodchem.2007.04.038
  15. Y. S. Duan, M. A. Kim, H. S. Kim, S. H. Jang and D. S. Kang, Screening of antioxidant activity from exocarp of watermelon (Citrullus vulgaris L.), J. Environ. Sci. Int., 23, 1233 (2014). https://doi.org/10.5322/JESI.2014.23.7.1233
  16. A. Meda, C. E. Lamien, M. Romito, J. Millogo and O. G. Nacoulma, Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity, Food Chem., 91, 571 (2005). https://doi.org/10.1016/j.foodchem.2004.10.006
  17. S. Gorinstein, J. Drzewiecki, H. Leontowicz, M. Leontowicz, K. Najman, Z. Jastrzebski, Z. Zachwieja, H. Barton, B. Shtabsky, E. Katrich and S. Trakhtenberg, Comparison of the bioactive compounds and antioxidant potentials of fresh and cooked polish, Ukrainian, and Israeli garlic, J. Agric. Food Chem., 53, 2726 (2005). https://doi.org/10.1021/jf0404593
  18. C. Kaur and H. C. Kapoor, Anti-oxidant activity and total phenolic content of some Asian vegetables, Int. J. Food Sci. Technol., 37, 153 (2002). https://doi.org/10.1046/j.1365-2621.2002.00552.x
  19. F. F. Benzie and J. J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Anal. Biochem., 239, 70 (1996). https://doi.org/10.1006/abio.1996.0292
  20. R. J. Robbins, Phenolic acids in foods: an overview of analytical methodology, J. Agric. Food Chem., 51, 2866 (2003). https://doi.org/10.1021/jf026182t
  21. P. Stratil, B. Klejdus and V. Kuban, Determination of Total Content of Phenolic Compounds and Their Antioxidant Activity in Vegetables-Evaluation of Spectrophotometric Methods, J. Agric. Food Chem., 54, 607 (2006). https://doi.org/10.1021/jf052334j
  22. Y. S. Velioglu, G. Mazza, L. Gao and B. D. Oomah, Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products, J. Agric. Food Chem., 46, 4113 (1998). https://doi.org/10.1021/jf9801973
  23. N. C. Cook and S. Samman, Flavonoids- Chemistry, metabolism, cardioprotective effects, and dietary sources, Nutri. Biochem., 7, 66 (1996). https://doi.org/10.1016/0955-2863(95)00168-9
  24. P. C. H. Hollman, M. G. L. Hertog and M. B. Katan, Analysis and health effects of flavonoids, Food Chem., 57, 43 (1996). https://doi.org/10.1016/0308-8146(96)00065-9
  25. B. Bozin, N. Mimica-Dukic, I. Samojlik, A. Goran and R. Igic, Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae), Food Chem., 111, 925 (2008). https://doi.org/10.1016/j.foodchem.2008.04.071
  26. L. S. Lai, S. T. Chou and W. W. Chao, Studies on the antioxidative activities of hsian-tsao (Mesona procumbens Hemsl) leaf gum. J. Agric. Food Chem., 49, 963 (2001). https://doi.org/10.1021/jf001146k
  27. A. Braca, C. Sortino, M. Politi, I. Morelli and J. Mendez, Antioxidant activity of flavonoids from Licania licaniaeflora, J. Ethnopharmacol., 79, 379 (2002). https://doi.org/10.1016/S0378-8741(01)00413-5
  28. V. Roginsky and E. A. Lissi, Review of methods to determine chain-breaking antioxidant activity in food, Food Chem., 92, 235 (2005). https://doi.org/10.1016/j.foodchem.2004.08.004
  29. Y. Hanasaki, S. Ogawar and S. Fukui, The correlation between active oxygen scavenging and antioxidative effects of flavonoids, Free Radical Biol. Med., 16, 845 (1994). https://doi.org/10.1016/0891-5849(94)90202-X
  30. A. A. Elzaawely, T. D. Xuan and S. Tawata, Antioxidant and Antibacterial Activities of Rumex japonicus HOUTT. Aerial Parts, Biol. Pharm. Bull., 28, 2225 (2005). https://doi.org/10.1248/bpb.28.2225
  31. Y. S. Queiroz, E. Y. Ishimoto, D. H. M. Bastos, G. R. Sampaio and E. A. F. S. Torres, Garlic (Allium sativum L.) and ready-to-eat garlic products: In vitro antioxidant activity, Food Chem., 115, 371 (2009). https://doi.org/10.1016/j.foodchem.2008.11.105
  32. F. Taji, H. Shirzad, K. Ashrafi, N. Parvin, S. Kheiri, A. Namjoo, A. Asgari, R. Ansari and M. Rafieian-kopaei, A comparison between the antioxidant strength of the fresh and stale allium sativum (garlic) extracts, Zahedan J. Res. Med. Sci., 14, 25 (2012).
  33. X. N. Lu, C. F. Ross, J. R. Powers, D. E. Aston and B. A. Rasco, Determination of total phenolic content and antioxidant activity of garlic (Allium sativum) and elephant garlic (Allium ampeloprasum) by attenuated total reflectance_fourier transformed infrared spectroscopy, J. Agric. Food Chem., 59, 5215 (2011). https://doi.org/10.1021/jf201254f

Cited by

  1. 추출 용매에 따른 아사이 베리(Euterpe oleracea Mart.)의 생리활성 및 항산화 활성 비교 vol.33, pp.4, 2016, https://doi.org/10.12925/jkocs.2016.33.4.741
  2. Effect of Allium sativum methanol extract in amelioration of arsenic-induced toxicity in Swiss albino mice vol.2, pp.1, 2016, https://doi.org/10.1016/j.phyplu.2021.100192