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Cultural Characteristics of Fungi Strains isolated from Korean Nuruk

전통누룩에서 분리한 양조용 곰팡이의 배양 특성 연구

  • Mun, Ji-Young (Fermented Food Science Division, Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Baek, Seong-Yeol (Fermented Food Science Division, Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration) ;
  • Park, Hye-Young (Crop Post-Harvest Technology Division, National Institute of Crop Science, Rural Development Administration) ;
  • Ro, Hyeon-Su (Dept. of Microbiology and Research Institute of Life Science, Gyeongsang National University) ;
  • Yeo, Soo-Hwan (Fermented Food Science Division, Dept. of Agrofood Resources, National Institute of Agricultural Science, Rural Development Administration)
  • 문지영 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 백성열 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 박혜영 (국립식량과학원 중부작물부 수확후이용과) ;
  • 노현수 (경상대학교 생명과학부) ;
  • 여수환 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과)
  • Received : 2016.01.25
  • Accepted : 2016.03.17
  • Published : 2016.04.30

Abstract

We analyzed the cultural characteristics of isolated brewing fungi for developing fermentation starters. In a previous study, we collected 87 domestic nuruk, from which 481 fungi strains were isolated and 11 were selected showing improved productivity. After culturing these 11 fungi strains in several types of media, temperatures, carbon and nitrogen sources, Rhizopus sp. grew well in MEA, ME20S, PDA medium while Aspergillus sp. grew well in ME20S and YES. Both Rhizopus sp. and Aspergillus sp. survived well at optimal growth temperatures of 30 and $37^{\circ}C$. Rhizopus sp. utilized lactose, glucose and peptone sources while Aspergillus sp. utilized glucose, mannose, fructose and yeast extract sources. ${\alpha}-Amylase$ activity was excellent in L. ramosa CN044, R. oryzae 82-7(MEB), R. oryzae CN174 and A. oryzae 58-11(WEB) culture extracts. This study suggests that R. delemar 26-4, 58-8 and A. oryzae 78-5, 37-7 might be appropriate fungi strains for fermentation starters based on development of large fungi bodies and their good enzyme activities.

Keywords

References

  1. Choi YH, Lee JE, Kim EM, Park SY (2012) Quality changes of steamed rice bread with addition of active gluten and rice Nuruk. Korean J Food Nutr 25(2): 253-258. https://doi.org/10.9799/ksfan.2012.25.2.253
  2. http://www.bioin.or.kr/board.do?cmd=view&bid=system&num=229722. Accessed on August 27, 2012.
  3. http://www.kiet.re.kr/kiet_web/?sub_num=40&state=view&idx=34784. Accessed on Oct. 29, 2012.
  4. Huh CK, Kim SM, Kim YD (2014) Comparison for enzymic activity of Nuruk and quality properties of Yakju by different fungi. Korean J Food Preserv 21(4): 573-580. https://doi.org/10.11002/kjfp.2014.21.4.573
  5. Hwang JY, Choi SH, Lee SK, Kim SM (2009) Optimal conditions for the production of salt-tolerant protease from Aspergillus sp. 101 and its characteristics. J Korean Soc Food Sci Nutr 38(11): 1612-1617. https://doi.org/10.3746/jkfn.2009.38.11.1612
  6. IIT (2014) Effect of Nagoya protocol on industry. Trade Brief, Korea. No. 59, Institute for International Trade, Seoul. pp 1-8.
  7. Im SY, Baek CH, Baek SY, Park HY, Choi HS, Choi JH, Jeong ST, Shin WC, Park HD, Yeo SH (2014) Quality characteristics of Takju according to different rice varieties and mixing ratio of Nuruk. Korean J Food Preserv 21(6): 892-902. https://doi.org/10.11002/kjfp.2014.21.6.892
  8. Jeong JH, Chai HS, Lee YH, Kim JM, Lee JH (2015) Quality characteristics of Takju, Yakju, spirit made by cereal Nuruks. Korean J Culinary Research 21(1): 267-280.
  9. Nam TJ, Pyeun JH, Kim DS, Kim HR (1999) Medium composition of Aspergillus oryzae PF for the production of proteolytic enzyme. Korean J Appl Microbiol Biotechnol 27(5): 404-409.
  10. Noh JM, Choi JH, Jung ST, Yeo SH, Park JW, Lee JW, Choi HS (2013) Mycelial production and amylase activity of fungi for brewing in different submerged culture conditions. J East Asian Soc Diet Life 23(6): 833-838.
  11. OH DS (2004) Improvement of Aspergillus oryzae by mutagenesis and optimization of its sporulation conditions. Woosuk University. Wanju. pp 21-41.
  12. Park JH, Yeo SH, Choi JH, Jeong ST, Choi HS (2012) Production of Makgeolli using rice treated with Gaeryang-Nuruk (for non-steaming process) extract. Korean J Food Preserv 19(1): 144-152. https://doi.org/10.11002/kjfp.2012.19.1.144
  13. Park JY, Kim JG (2009) Inhibitory effect of garlic on the growth of Aspergillus parasiticus. J Env Hlth Sci 35(2): 95-99.
  14. Park SH, Koo KB, Park JS, Hong YK (2001) Optimal culture conditions of fungal pathogen YK101 for mycelium production. Korean Soc Biotechnol Bioeng pp 471-474.
  15. So MH (1991) Improvement in the quality of Takju by the com- bined use of Aspergillus kawachii and Aspergillus oryzae. Korean J Food Nutr 4(2): 115-124.
  16. So MH (1999) Characteristics of a modified Nuruk made by inoculation of traditional Nuruk microorganisms. Korean J Food Nutr 12(3): 219-225.
  17. So MH, Lee JW (1996) Takju brewing by combined use of Rhizopus japonicus-Nuruk and Aspergillus oryzae-Nuruk. J Korean Soc Food Nutr 25(1): 157-162.
  18. So MH, Lee YS, Noh WS (1999) Improvement in the quality of Takju by a modified Nuruk. Korean J Food Nutr 12(4): 427-432.
  19. Woo SM, Shin JS, Seong JH, Yeo SH, Choi JH, Kim TY, Jeong YJ (2010) Quality characteristics of brown rice Takju by different Nuruks. J Korean Soc Food Sci Nutr 39(2): 301-307. https://doi.org/10.3746/jkfn.2010.39.2.301
  20. Yeo SH, Baek SY (2014) Application technology of useful brewing microorganisms. Final Report, Korea. National Institute of Agricultural Science, Jeonju. pp 8-52.

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