Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Development of Drainage Water Disinfection System by Electric Shock in Recirculating Soilless Culture

순환식 수경재배에서 배액의 전기충격살균법 연구

  • 이문행 (충청남도농업기술원) ;
  • 김성은 (써브스트라투스코리아) ;
  • 이상돈 (써브스트라투스코리아) ;
  • 이재은 (상명대학교 식물식품공학과) ;
  • 김학선 (상명대학교 식물식품공학과) ;
  • 조숙경 (상명대학교 식물식품공학과) ;
  • 심상연 (경기도농업기술원) ;
  • 김영식 (상명대학교 식물식품공학과)
  • Received : 2016.03.08
  • Accepted : 2016.03.23
  • Published : 2016.03.31
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Abstract

This study was conducted to develop the disinfection system using electric shock for recycled nutrient solution in recycling soilless culture. Stainless steel (SUS 316) was found as the most appropriate electrode material for electrical disinfection system from the view of high electrical conductivity, low electric resistance, and low price. There were no changes in nutritional elements when the electric shock passed through the nutrient solution by stainless steel electrode. The amount of electric current increased with width than thickness of the electrode. The farther the distance between the electrodes was increased the time to reach out the aimed amount of current. The electric shock was applied to Ralstonia solanacearum and Fusarium oxysporum as representative bacteria and also fungi. Any of those pathogens were killed with the percentage of higher than 97% in the condition of 15VDC or 24VDC.

본 연구는 의료살균기술의 하나인 전극을 이용한 전기충격 살균법을 순환식 수경재배의 배양액 재순환을 위한 배액 살균기술로 활용하고, 배액의 전기살균소독시스템을 구축하고자 할 때, 살균소독 효과가 높으며, 친환경적이고 경제적인 배양액 살균소독기술의 개발 및 전기살균소독시스템에서 사용될 전극의 최적 조건을 구명하고 전기소독의 가능성을 확인하기 위해 수행되었다. 전기살균소독시스템 구축 시 적정 전극소재 구명을 위해 금속 전도체의 특성 조사 및 전기실험을 실시하여 배액의 pH와 EC변화유무와 침전물 발생여부 및 배액의 원소변화 유무를 분석하였다. 새로이 개발된 전기살균소독시스템 구축 시 가장 적합한 금속 전도체 전극소재로는 전기전도도가 높고, 저항이 적으며, 소재의 수급이 용이하고, 가격이 저렴한 스테인리스 스틸임을 확인하였으며. 또한 스테인리스 스틸을 전극으로 사용하였을 때, 전기를 공급하기 전과 24V 이내의 전기를 공급한 후의 배양액내 원소변화는 거의 없는 것으로 분석되었다. 전극의 두께보다는 넓이가 증가함에 따라 전류의 양이 증가하였으며 전극의 거리가 멀수록 목표 전류량에 도달하는 시간이 증가하였다. 적합한 전류량에 따른 주요 병원균의 살균력을 조사한 결과 대표적 세균병인 풋마름병의 원인균인 Ralstonia solanacearum가 전류 15V-3A 170초에서 97%가 사멸되는 것을 확인하였으며 곰팡이병인 Fusarium oxysporum은 24V-10A에서 98%의 살균력을 보였다.

Keywords

References

  1. Akahori, Y., M. Murakami, and S. Hoshi. 2000. Sterilization effects and prevention of hospital-acquired infections by ozonated water. J. Jpn. Soc. Intensive Care Med 7: 3-10. https://doi.org/10.3918/jsicm.7.3
  2. Barak, Y. and J. van Rijn. 2000. Atypical polyphosphate accumulation by the denitrifying bacterium Paracoccus denitrificans. Appl. Environ. Microbiol. 66: 1209-1212. https://doi.org/10.1128/AEM.66.3.1209-1212.2000
  3. Bishay, J. M., and P. Leonard. 2003. Percutaneous electrical therapy system for minimizing electrode insertion discomfort. U.S. Patent No. 6,516,226. Washington, DC: U.S. Patent and Trademark Office.
  4. Bolton, J. R. 2000. Calculation of ultraviolet fluence rate distribution in an annular reactor: significance of refraction and reflection. Water Res. 34: 3315-3324. https://doi.org/10.1016/S0043-1354(00)00087-7
  5. Bukhari, Z., T. M. Hargy, J. R. Bolton, B. Dussert, and J. L. Clancy. 1999. Medium-pressure UV for oocyst inactivation. J. Am. Water Works Assoc. 91: 86-94.
  6. Cho, H.Y., L.Y. Zhang, and S.R. Kleeberger. 2001. Ozoneinduced lung inflammation and hyperreactivity are mediated via tumor necrosis factor-alpha receptors. Am. J. Physiol. 280: 537-546.
  7. Clancy, J. L., Z. Bukhari, T. M. Hargy, J. R. Bolton, B. W. Dussert, and M. M. Marshall. 2000. Using UV to inactivate Cryptosporidium. J. Am. Water Works Assoc. 92: 97-104. https://doi.org/10.1002/j.1551-8833.2000.tb09008.x
  8. Craik, S.A., D. Weldon, G.R. Finch, J.R. Bolton, and M. Belosevic. 2001. Inactivation of Cryptosporidium oocysts using medium- and low-pressure ultraviolet radiation. Water Res. 35: 1387-1398. https://doi.org/10.1016/S0043-1354(00)00399-7
  9. Fakhri, O., and M.A. Amin. 1987. The effect of low-voltage electric therapy on the healing of resistant skin burns. J. Burn Care & Research 8: 15-18. https://doi.org/10.1097/00004630-198701000-00003
  10. Gehl, J. 2003. Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research. Acta Physiologica Scandinavica 177:437-447. https://doi.org/10.1046/j.1365-201X.2003.01093.x
  11. Giancoli, D.C. 1995. Physics: Principles with Applications (4th ed.). London: Prentice Hall.
  12. Giancoli, D.C. 2009. "25. Electric Currents and Resistance". In Jocelyn Phillips. Physics for Scientists and Engineers with Modern Physics (4th ed.). Upper Saddle River, New Jersey: Prentice Hall. p. 658.
  13. Grguric, G., S.S. Wetmore, and R.W. Fournier. 2000. Biological denitrification in a closed seawater system. Chemosphere 40: 549-555. https://doi.org/10.1016/S0045-6535(99)00311-2
  14. Griffiths, D. 1999. "7. Electrodynamics". In Alison Reeves. Introduction to electrodynamics (3rd ed.). Upper Saddle River, New Jersey: Prentice Hall. p. 286.
  15. Hanasak, H. 2000. Clinical evaluation of cataract surgery using ozone water as an ocular surface disinfectant. Jpn. J. Ophthalmic Surg. 13: 456-458.
  16. Hoyer, O. 1998. Testing performance and monitoring of UV systems for drinking water disinfection. Water Supply 16: 424-429.
  17. Jin, H.J., J. Chen, V. Karageorgiou, G.H. Altman, and D.L. Kaplan. 2004. Human bone marrow stromal cell responses on electrospun silk fibroin mats. Biomaterials 25: 1039-1047. https://doi.org/10.1016/S0142-9612(03)00609-4
  18. Kalisvaart, B.F. 2001. Photobiology effects of polychromatic medium pressure UV lamps. Water Sci. Technol. 43: 191-197.
  19. Katoh, Y., N. Horiba, T. Matsumoto, and H. Nakamura. 2012. Study on the use of an ozone gel for endodontic therapy : bactericidal and cytotoxic effects. J. Jpn. Endo. Assoc. 33: 20-28.
  20. Kim, K.H., L. Jeong, H.N. Park, S.Y. Shin, W.H. Park, and S.C. Lee. 2005. Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration. J. Biotechnol. 120: 327-339. https://doi.org/10.1016/j.jbiotec.2005.06.033
  21. Lee, D.U., I.S. Lee, Y.D. Choi, and J.H. Bae. 2001. Effects of external carbon source and empty bed contact time on simultaneous heterotrophic and sulfur-utilizing autotrophic denitrification. Process Biochem. 36: 1215-1224. https://doi.org/10.1016/S0032-9592(01)00163-7
  22. Lee, H.C. 2014. Necessity and challenge project of closed-system in the hydroponics. Kor. J. Hort. Sci. Technol. 32 (Suppl II): 31-32.
  23. Lee, P.Y., S. Chesnoy, and L. Huang. 2004. Electroporatic delivery of TGF-${\beta}1$ gene works synergistically with electric therapy to enhance diabetic wound healing in db/db mice. J. Investigative dermatology 123: 791-798. https://doi.org/10.1111/j.0022-202X.2004.23309.x
  24. Lee, S.D. 2014. Recycling soilless systems. Kor. J. Hort. Sci. Technol. 32 (Suppl II): 32-33.
  25. Lee, S.R., T.H. Kang, C.S. Han, M.M. Oh. 2014. Air anions improve growth and mineral concentration of kale in a plant factory. Kor. J. Hort. Sci. Technol. 32 (Suppl II): 41-42.
  26. Lens, P., L.H. Pol, P. Wilderer, and T. Asano. 2005. Water recycling and resource recovery in industry. Water Intelligence Online, IWA Publishing e-Book, p. 661-664.
  27. Lin, W.Z., S.S. Lee, and W.T. Cheung. 2009. Efficient expression of foreign genes in CHO DHFR cells by electroporation. Biologicals 37: 277-281. https://doi.org/10.1016/j.biologicals.2009.03.003
  28. Linden, K. G., and J. L. Darby. 1997. Estimating effective germicidal dose form medium pressure UV lamps. J. Environ. Eng. 123: 1142-1149. https://doi.org/10.1061/(ASCE)0733-9372(1997)123:11(1142)
  29. Lippman, M. 1989. Health effects of ozone. J. Am. Air Pollut Control Assoc. 39: 672-695.
  30. Malley, J.O. 1992. Schaum's outline of theory and problems of basic circuit analysis. p. 19. McGraw-Hill Professional.
  31. Matsuki, N., T. Ishikawa, Y. Imai, and T. Yamaguchi. 2008. Low voltage pulses can induce apoptosis. Cancer letters 269: 93-100. https://doi.org/10.1016/j.canlet.2008.04.019
  32. Meinel, L., S. Hofmann, O. Betz, R. Fajardo, H.P. Merkle, and R. Langer. 2006. Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. Biomaterials 27: 4993-5002. https://doi.org/10.1016/j.biomaterials.2006.05.021
  33. Menasveta, P., T. Panritdam, P. Sihanonth, S. Powtongsook, B. Chuntapa, and P. Lee. 2001. Design and function of a closed, recirculating seawater system with denitrification for the culture of black tiger shrimp broodstock. Aquacult. Eng. 25: 35-39. https://doi.org/10.1016/S0144-8609(01)00069-3
  34. Metcalf, L., H.P. Eddy. 2004. Wastewater engineering : treatment, disposal, and reuse. 4th ed. McGraw-Hill, New York, USA. p. 182-220.
  35. Mofidi, A. A., H. Baribeau, P. A. Rochelle, R. De Leon, B. M. Coffey, and J. F. Green. 2001. Disinfection of Cryptosporidium parvum with polychromatic UV light. J. Am. Water Works Assoc. 93: 95-109.
  36. Noah, E.M., J. Chen, X. Jiao, I. Heschel, and N. Pallua. 2002. Impact of sterilization on the porous design and cell behavior in collagen sponges prepared for tissue engineering. Biomaterials 23: 2855-2861. https://doi.org/10.1016/S0142-9612(01)00412-4
  37. Patermarakis, G. and E. Fountoukidis. 1990. Disinfection of water by electrochemical treatment. Water Research 24:1491-1496. https://doi.org/10.1016/0043-1354(90)90083-I
  38. Patterson, M.A. 1976. Effects of neuro-electric therapy (NET) in drug addiction: interim report. UN Bull. Narc, 28: 55-62.
  39. Sakai, D., Y. Makita, K. Masuno, S. Fujiwara, J. Okazaki, and P. Wang. 2014. Local hemostatic effect of aqueous ozone in cutting wound surface. J. Hard. Tissue Biol. 23: 245-248. https://doi.org/10.2485/jhtb.23.245
  40. Schlegal, H.G., 1992.Allegemine Mikrobiologie. Thieme-Verlag, Stuttgart, New York, USA : p. 634
  41. Serway, R.A. 1998. Principles of physics (2nd ed). Fort Worth, Texas; London: Saunders College Pub. p. 602.
  42. Shnel, N., Y. Barak, T.Y., Ezer, Z. Dafni, and J. van Rijn. 2002. Design and performance of a zero-discharge tilapia recirculating system. Aquacult. Eng. 26: 191-203. https://doi.org/10.1016/S0144-8609(02)00013-4
  43. Shin, D.H. and Y.T. Lee. 2005. Preparation and characterization of sewage sterilization for electrodeless UV lamp. J. Kor. Ind. Eng. Chem. 16: 570-575.
  44. Sofia, S., M.B. McCarthy, G. Gronowicz, and D.L. Kaplan. 2001. Functionalized silk-based biomaterials for bone formation. J. Biomed Mater Res. 54: 139-148. https://doi.org/10.1002/1097-4636(200101)54:1<139::AID-JBM17>3.0.CO;2-7
  45. Sommer, R., M. Lhotsky, T. Haider, and A. Cabaj. 2000. UV inactivation, liquid-holding recovery, and photoreactivation of Escherichia coli O157 and other pathogenic Escherichia coli strains in water. J. Food Prot. 63: 1015-1020. https://doi.org/10.4315/0362-028X-63.8.1015
  46. van Rijn, J.V., Y. Tal, and H.J. Schreier. 2006. Denitrification in recirculating system: Theory and applications. Aquacult. Eng. 34: 364-376. https://doi.org/10.1016/j.aquaeng.2005.04.004
  47. Wohanka, W., 1992. Slow sand filtration and UV radiation; low-cost techniques for disinfection of recirculating nutrient solutions or surface water. In: Proc 8th Internat. Congr. Soilless Culture. ISOSC, Wageningen, The Netherlands. pp. 497-511.