Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
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The Content of Total Polyphenol, Total Flavonoid and the Antioxidant Activity in Different Origin and Parts of Moringa Olerifera Cultivated in Cheorwon

모링가(Moringa olerifera)의 부위별 총페놀, 총플라보노이드 함량 및 항산화 활성

  • Lee, Jung Sun (Dept. of Food and Nutrition, Hallym Polytechnic University) ;
  • Lee, Myung Heon (Dept. of Food and Nutrition, Hallym Polytechnic University) ;
  • Park, Chun Il (Cheorwon Agricultural Technology Center) ;
  • Lee, Hee Jong (Cheorwon Agricultural Technology Center) ;
  • Hong, Hyun Dong (Cheorwon Agricultural Technology Center) ;
  • Lee, Bo Duck (Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Sung, In Je (Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Chang, Kwang Jin (Korea National College of Agriculture & Fisheries) ;
  • Park, Cheol Ho (Institute of Bioscience & Biotechnology, Kangwon National University)
  • 이정선 (한림성심대학교 식품영양학과) ;
  • 이명헌 (한림성심대학교 식품영양학과) ;
  • 박춘일 (철원군농업기술센터) ;
  • 이희종 (철원군농업기술센터) ;
  • 홍현동 (철원군농업기술센터) ;
  • 이보덕 (강원대학교 의생명과학연구소) ;
  • 성인제 (강원대학교 의생명과학연구소) ;
  • 장광진 (한국농수산대학교 특용작물학과) ;
  • 박철호 (강원대학교 의생명과학연구소)
  • Received : 2016.05.18
  • Accepted : 2016.09.23
  • Published : 2016.09.30
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Abstract

This study was conducted to investigate the total polyphenol, total flavonoid and the antioxidant activity in different origin and parts of Moringa olerifera cultivated in Cheorwon. The extraction yield from leaf, root and stem were 25.5, 23.0, 12.3%, respectively. The total polyphenol content was high in the leaf extracts (15.77 mg/g) followed by root extracts (11.23 mg/g) and stem extracts (3.50 mg/g) but the difference of origin was not observed. The total flavonoid content was high in the leaf extracts (14.78 mg/g) followed by stem (1.36 mg/g) and root (1.14 mg/g). The total flavonoid content of Philippines and Thailand leaf were statistically high compared to india leaf. The DPPH radical scavenging activity was high in the leaf extracts at the 1 mg/ml, 0.25 mg/ml but the difference of origin was not observed. The DPPH radical scavenging activity may be attributed to the presence of several compounds such as polyphenol and flavonoid. Hence consumption of diet supplemented with Moringa olerifera leaves could protect the human against diseases induced by oxidative stress.

인도, 필리핀, 태국에서 생산된 모링가(Moringa oleifera) 종자를 강원도 철원농업기술센터에서 2015년 5월 파종하고 11월 수확하여 잎, 줄기, 뿌리로 나누어 건조, 분말화 하여 시료로 사용하였다. 시료를 70% 에탄올 용액으로 추출하여 추출 수율과 항산화력과 관련이 있는 총 폴리페놀 함량, 총 플라보노이드 함량을 구하였고 항산화 활성은 DPPH를 이용한 자유라디칼 소거능으로 확인하였으며 결과는 다음과 같았다. 1. 모링가 잎, 줄기, 뿌리 건조분말을 70% 에탄올로 추출한 수율은 잎(25.5%), 뿌리(23.0%), 줄기(12.3%) 순으로 나타났으며 종자의 지역별 차이는 크지 않았다. 2. 모링가 잎, 줄기, 뿌리에 함유된 총 폴리페놀 함량의 평균치는 잎(15.77 mg/g), 뿌리(11.23 mg/g), 줄기(3.50 mg/g) 순으로 잎에 가장 많이 함유되어있었으며 부위별로는 유의적인 차이를 보였으나 지역별 차이는 보이지 않았다. 3. 모링가 잎, 줄기, 뿌리에 함유된 총 플라보노이드 함량의 평균치는 잎(14.78 mg/g), 줄기(1.36 mg/g), 뿌리(1.14mg/g) 순이었으며 잎의 경우만 필리핀산과 태국산이 인도산보다 유의적으로 많았으며 줄기나 뿌리에서는 지역별로 총 플라보노이드 함량 차이를 보이지 않았다. 4. 모링가 잎, 줄기, 뿌리의 DPPH 자유라디칼 소거능은 시료농도와 비례적인 반응을 보였으며, 부위별로는 잎> 뿌리> 줄기 순으로 높았으며 지역적인 차이는 미미하였다. 모링가의 DPPH 자유라디칼 소거능은 시료의 플라보노이드 함량보다는 총 폴리페놀 함량과 더 관련이 있었다.

Keywords

Acknowledgement

Supported by : 강원대학교

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