Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Changes in phytoestrogen contents and antioxidant activities during fermentation of soybean-powder milks prepared from different soybean cultivars by Lactobacillus plantarum P1201

Lactobacillus plantarum P1201에 의한 콩 품종별 콩-분말 두유 발효 과정에서의 식물성 에스트로젠 함량과 항산화 활성의 변화

  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Haque, Md. Azizul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Jin Hwan (Division of Research Development and Education, National Institute of Chemical Safety (NICS), Ministry of Environment) ;
  • Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Lee, Byong Won (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Lee, Yu-Young (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Lee, Choonwo (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Kim, Byung Joo (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Park, Ji-Yong (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Sim, Eun-Yeong (Department of Central Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Lee, Dong Hoon (Department of Anatomy and Convergence Medical Science, School of Medicine, Gyeongsang National University) ;
  • Ko, Jong Min (Department of South Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Kim, Hyun Tae (Department of South Area, Crop Science, National Institute of Crop Science (NICS), Rural Development Administration (RDA)) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • Received : 2015.10.05
  • Accepted : 2016.06.21
  • Published : 2016.06.30
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Abstract

This study evaluated the changes of phytoestrogen contents and antioxidant activities of soybean-powder milk (SPM) prepared from yellow soybean during fermentation with Lactobacillus plantarum P1201. In consequence, the levels of total phenolic and isoflavone-aglycone contents, ABTS and DPPH radical-scavenging activities, and FRAP assay values increased, while isoflavone-glycoside contents decreased during fermentation. The highest levels of daidzein, glycitein, and genistein were present in the Daepung SPM at concentrations of 177.92, 20.64, and $106.14{\mu}g/g$, respectively after 60 h of fermentation. Moreover, Daepung SPM showed the highest DPPH radical-scavenging activity of 48.54%, an ABTS radical-scavenging activity of 99.25%, and a FRAP assay value of 0.84 at the end of fermentation. The fermented Daepung SPM possessed highest isoflavone aglycone contents and antioxidant activities, which can be utilized for the development of functional foods.

본 연구는 노란콩 8품종(새단백, 대원, 대풍, 늘찬, 태광, 선유, 황금, 및 대망)에 대해 Lactobacillus plantarum P1201 균주를 이용하여 콩-분말 두유 발효 중 식물성 에스트로겐 및 항산화 활성 변화를 측정하였다. 그 결과, 발효가 진행되는 동안 isoflavone-glycoside는 감소하였고, total phenolic 및 isoflavoneaglycone 함량과 DPPH와 ABTS 라디칼 소거활성 및 FRAP 환원력은 증가하였다. 특히 대풍콩-분말 두유는 발효 60시간 후 daidzein, glycitein, 및 genistein 함량이 각각 177.92, 20.64, 및 $106.14{\mu}g/g$으로 다른 콩 품종들보다 가장 높은 것으로 나타났으며 또한 대풍콩-분말 두유는 발효 후 DPPH 라디칼 소거활성은 48.54%, ABTS 라디칼 소거활성은 99.25% 및 FRAP 환원력은 0.84로 가장 높게 나타났다. 따라서 대풍콩-분말 두유는 aglycone 함량이 높고 우수한 항산화 활성을 나타내므로 기능성 식품 개발에 이용될 수 있을 것으로 기대된다.

Keywords

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