Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
권호 표지
DOI QR코드

DOI QR Code

The Anti-bacterial Activity of Eco-friendly Farming Material based on Chinese Nut-gall Extraction on Acidovorax citrulli

오배자 추출물 유래 친환경제제의 세균성과실썩음병균에 대한 항세균활성

  • 서태진 (전북대학교 농생물학과) ;
  • 양수정 (전북대학교 농생물학과) ;
  • 이봉춘 (국립식량과학원) ;
  • 김강민 (전북대학교 생명공학부) ;
  • 이귀재 (전북대학교 생명공학부) ;
  • 주호종 (전북대학교 농생물학과, 식물의학연구센터, 농업과학기술연구소)
  • Received : 2016.06.17
  • Accepted : 2016.07.21
  • Published : 2016.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Bacterial fruit blotch (BFB) is one of most important diseases in Cucurbitaceae due to infection of Acidovorax citrulli, causing huge economic losses damage worldwide. This seedborn disease spread rapidly at period of high temperature and humidity. The eco-friendly farming is getting popular. So far there was no effective agent to control BFB in eco-friendly farming. Therefore, effect of the material based on chinese nut-gall extract with antibacterial activity against BFB to was tested against A. citrulli. Different hosts showed various symptoms of BFB. Liquid formulation among exhibited most effective anti-bacterial activity on A. citrulli. Pot experiment in greenhouse showed the potential as an control agent of BFB in cucurbits. The treatment of material based on chinese nut-gall extract showed the positive effect on survival of the watermelon seedling and on the length of the cucumber seedling treated with A. citrulli. We cautiously conclude that the material based on chinese nut-gall extract used in this study may be good agents against major diseases of cucurbits in the future even though it is require to be tested with more study on field test.

세균성과실썩음병(BFB)은 Acidovorax citrulli가 원인균으로서 전 세계적으로 박과채소에 큰 손실을 야기하며 고온다습한 조건에서 급속도로 진전되는 종자 병이다. 요즘 친환경재배가 인기를 얻어가고 있으나 BFB를 효율적으로 방제할 수 있는 적당한 방제제가 없는 실정에서 오배자 추출물 기반 약제의 A. citrulli에 대한 황세균활성을 시험하였다. 먼저 식물마다 다른 다양한 BFB 증상이 나타났다. 오배자 추출물 기반 약제의 액상 형태의 방제제는 높은 항세균활성을 보였으며, 온실내 포트실험 또한 오배자 추출물 기반 약제의 BFB 방제에 대한 잠재적 효과성을 보였다. 오배자 추출물 기반 약제 처리는 수박 유묘의 생존율과 오이의 유묘 길이에서 긍정적인 효과를 보였다. 따라서 이 연구에서 사용한 오배자 추출물 기반 약제에 대하여 추가적인 연구가 이루어진다면 박과채소인 BFB를 효과적으로 방제할 수 있는 가능성이 아주 높은 물질로 생각이 된다.

Keywords

References

  1. Cheng, A. H., Y. L. Hsu, T. C. Huang, and H. L. Wang. 2000. Susceptibility of cucurbits to Acidovorax avenae subsp. citrulli and control of fruit blotch on melon. Plant Pathol. Bull. 9:151-156.
  2. Dutta, B., H. Scherm, R. D. Gitaitis, and R. R. Walcott. 2012. Acidovorax citrulli seed inoculum load affects seedling transmission and spread of bacterial fruit blotch of watermelon under greenhouse conditions. Plant Dis. 96: 705-711. https://doi.org/10.1094/PDIS-04-11-0292
  3. FAOSTAT. 2013. FAOSTAT, available at http://faostat3.fao.org/home/E accessed on March 2016.
  4. Hopkins, D. L. and C. M. Thomson. 2002. Seed transmission of Acidovorax avenae subsp. citrulli in cucurbits. HortSci. 37: 924-926.
  5. Hopkins, D. L., J. D. Cucuzza, and J. C. Watterson. 1996. Wet seed treatments for the control of bacterial fruit blotch of watermelon. Plant Dis. 30: 529-532.
  6. Hopkins, D. L. and N. C. Schenck. 1971. Bacterial leaf spot of watermelon caused by Pseudomonas lachrymans. Phytopathology 62: 542-545.
  7. Isakeit, T., M. C. Black, L. W. Barnes, and J. B. Jones. 1997. First report of infection of honeydew with Acidovorax avenae subsp. citrulli. Plant Dis. 81: 694-701.
  8. KOSTAT. 2013. KOSIS, available at http://kosis.kr/ accessed on March 2016.
  9. Latin, R. X. and D. L. Hopkins. 1995. Bacterial fruit blotch of watermelon: the hypothetical exam question becomes reality. Plant Dis. 79: 761-765. https://doi.org/10.1094/PD-79-0761
  10. Latin, R. X. and K. K. Fane. 1990 Bacterial fruit blotch of watermelon in Indiana. Plant Dis. 74: 331-335.
  11. Lessl, J. T., A. Fessehaie, and R. R. Walcott. 2007. Colonization of female watermelon blossoms by Acidovorax avenae subsp. citrulli and the relationship between blossom inoculum dosage and seed infestation. J. Phytopathol. 155: 114-121. https://doi.org/10.1111/j.1439-0434.2007.01204.x
  12. Martin, H. L., R. G. O'Brien, and D. V. Abbott. 1999 First report of Acidovorax avenae subsp. citrulli as a pathogen of cucumber. Plant Dis. 83: 965.
  13. Rane, K. K. and R. X. Latin. 1992. Bacterial fruit blotch of watermelon association of the pathogen with seed. Plant Dis. 76: 509-512. https://doi.org/10.1094/PD-76-0509
  14. Schaad, N. W., E. Postnikova, A. Sechler, L. E. Claflin, A. K. Vidaver, J. B. Jones, I. Agarkova, A. Ignatov, E. Dickstein, and B. A. Ramundo. 2008. Reclassification of subspecies of Acidovorax avenae as A. avenae (Manns, 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli (Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov. Syst. Appl. Microbiol. 31: 434-446. https://doi.org/10.1016/j.syapm.2008.09.003
  15. Seo, S. T., . H. Park, J. S. Lee, K. S. Han, and S. R. Cheong. 2006. Bacterial fruit blotch of melon caused by Acidovorax avenae subsp. citrulli. Res. Plant Dis. 12: 185-188. (In Korean) https://doi.org/10.5423/RPD.2006.12.3.185
  16. Shirakawa, T., S. Kikuchi, T. Kato, A. Abiko, and A. Kaiwa. 2000. Occurrence of watermelon bacterial fruit blotch in Japan. Jpn. J. Plant Pathol. 66: 223-231.
  17. Song, W. Y., H. M. Kim, I. Y. So, and Y. K. Kang. 1991. Pseudomonas pseudoalcaligenes subsp. citrulli: The causal agent of bacterial fruit blotch rot on watermelon. Kor. J. Plant Pathol. 7: 177-182. (In Korean)
  18. Walcott, R. R., R. D. Gitaitis, and A. C. Castro, 2003. Role of blossoms in watermelon seed infestation by Acidovorax avenae subsp. citrulli. Phytopathology 93: 528-534. https://doi.org/10.1094/PHYTO.2003.93.5.528