Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Synergistic Anti-bacterial Effects of Phellinus baumii Ethyl Acetate Extracts and β-Lactam Antimicrobial Agents Against Methicillin-Resistant Staphylococcus aureus

  • Hong, Seung Bok (Department of Clinical Laboratory Science, Chungbuk Health & Science University) ;
  • Rhee, Man Hee (Laboratory of Veterinary Physiology & Signaling, College of Veterinary Medicine and Stem Cell Research Therapeutic Institute, Kyungpook National University) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environmental & Bioresource Sciences, College of Environmental & Bioresource Science, Chonbuk National University) ;
  • Lim, Young Hoon (Department of Pathology, Chungbuk National University College of Medicine) ;
  • Song, Hyung Geun (Department of Pathology, Chungbuk National University College of Medicine) ;
  • Shin, Kyeong Seob (Department of Laboratory Medicine, Chungbuk National University College of Medicine)
  • Received : 2015.08.12
  • Accepted : 2015.11.20
  • Published : 2016.03.01
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Abstract

Background: The development of new drugs or alternative therapies effective against methicillin-resistant Staphylococcus aureus (MRSA) is of great importance, and various natural anti-MRSA products are good candidates for combination therapies. We evaluated the antibacterial activities of a Phellinus baumii ethyl acetate extract (PBEAE) and its synergistic effects with ${\beta}$-lactams against MRSA. Methods: The broth microdilution method was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the PBEAE. The PBEAE synergistic effects were determined by evaluating the MICs of anti-staphylococcal antibiotic mixtures, with or without PBEAE. Anti-MRSA synergistic bactericidal effects of the PBEAE and ${\beta}$-lactams were assessed by time-killing assay. An ELISA was used to determine the effect of the PBEAE on penicillin binding protein (PBP)2a production. Results: The MICs and MBCs of PBEAE against MRSA were 256-512 and $1,024-2,048{\mu}g/mL$, respectively. The PBEAE significantly reduced MICs of all ${\beta}$-lactams tested, including oxacillin, cefazolin, cefepime, and penicillin. However, the PBEAE had little or no effect on the activity of non-${\beta}$-lactams. Time-killing assays showed that the synergistic effects of two ${\beta}$-lactams (oxacillin and cefazolin) with the PBEAE were bactericidal in nature (${\Delta}log_{10}$ colony forming unit/mL at 24 hr: 2.34-2.87 and 2.10-3.04, respectively). The PBEAE induced a dose-dependent decrease in PBP2a production by MRSA, suggesting that the inhibition of PBP2a production was a major synergistic mechanism between the ${\beta}$-lactams and the PBEAE. Conclusions: PBEAE can enhance the efficacy of ${\beta}$-lactams for combined therapy in patients infected with MRSA.

Keywords

Acknowledgement

Grant : 임업현장 실용화 연구

Supported by : 전북대학교 산학협력단

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