Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant and Physiological Activities of Coriolus versicolor Fruit Body Crude Extracts

운지버섯(Coriolus versicolor) 자실체 조 추출물의 항산화 활성 및 생리활성 연구

  • Choi, Byeong Yong (Dept. of Naturopathic Medicine, Dongbang Culture Graduate Univ.) ;
  • Lee, Hyung H. (Dept. of Biological Sciences, Konkuk University)
  • 최병용 (동방문화대학원대학교 자연치유학과) ;
  • 이형환 (건국대학교 생명과학특성화학과)
  • Received : 2016.07.18
  • Accepted : 2016.08.11
  • Published : 2016.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this research, the total polyphenol and flavonoid contents in the hot water extract (HE) and methanol extract (ME) of the Coriolus versicolor fruiting body and their partial bioactivities were investigated from March, 2015 to February, 2016. For the analysis of the data, the software used was SPSS 2.0. The results indicate that the total polyphenol content in the HE (129.60 mg/dl) was 85,8 mg/dl higher than that in the ME (43.80 mg/dl). The total flavonoid content in the HE (30.50 mg/dl) was 9.5 mg/dl higher than that in the ME (20.90 mg/dl). The DPPH scavenging activities of the HE and ME at a concentration of 3 mg/ml were 41.03% and 16.36% higher than those at a concentration of 1 mg/ml. The tyrosinase inhibitory activities of the HE and ME at a concentration of 3 mg/ml were 8.29% and 10.32% higher than those at a concentration of 1 mg/ml. The elastase inhibitory activities of the HE and ME at a concentration of 3 mg/ml were 3.12% and 21.53% higher than those at a concentration of 1 mg/ml. The collagenase inhibitory activities of the HE and ME at a concentration of 3 mg/ml were 5.44% and 4.07% higher than those at a concentration of 1 mg/ml. In conclusion, these two kinds of extracts are judged to be useful as skin wrinkle relaxants, due to their anti-oxidative and bioactive functions.

본 연구는 운지버섯 자실체의 열수추출물(HE)과 메탄올 추출물(ME)의 총 polyphenol과 flavonoid 함량과 일부 생리활성을 2015년 3월부터 2016년 2월에 조사하였다. 자료 분석은 SPSS 2.0을 이용하였다. 연구결과는 HE의 총 polyphenol 함량(129.60 mg/dl)은 ME함량(43.80 mg/dl)보다 85,8 mg/dl가 더 높았으며, 열수에서 추출이 더 높았다. HE의 총 flavonoid 함량(30.50 mg/dl)은 ME함량(20.90 mg/dl)보다 9.5 mg/dl가 더 높았다. DPPH의소거활성은 HE 1 mg/ml에 비해서 3 mg/ml에서는 41.03%가, ME 1 mg/ml에 비해서 3 mg/ml에서는 16.36%로 더 높았으며, Tyrosinase저해활성은 HE 1 mg/ml에 비해서 3 mg/ml에서는 8.29%, ME 3 mg/ml에서는 10.32%가 더 높았고, Elastase 저해활성은 HE 1 mg/ml에 비해서 3 mg/ml에서는 3.12%가, ME에서는 21.53%가 더 높았다. Collagenase저해활성은 HE 1 mg/ml에 비해서 3 mg/ml에서는 5.44%가, ME에서는 4.07%로 더 높았다. 상기 효소활성은 모두 추출액농도증가에 비례하여 활성이 증가하였다. 결론적으로 상기의 두 종류의 추출물은 항산화 및 생리활성기능을 나타내어 피부의 주름의 완화제로서 유용하다고 판단되었다.

Keywords

References

  1. Y. K. Lee, Y. S. Song, K. K. Lee. "Evaluation of Antioxidant, Tyrosinase Inhibitory and Anti-inflammatory Activities of C. longa extracts", International. Journal of Beauty, Cultures and Arts, 1(1), pp. 2-8, 2012.
  2. S. C. Bae, D. Y. Kim, T. H. Kim. et al. "Nitric Oxide in Inflammatory Arthritis", Korean Journal of Medicine, 52(1), pp. 32-41, 1997.
  3. M. S. Kim, D. C. Lee, J. E., Hong, et al. "Antimicrobial Effects of Ethanol Extracts from Korean and Indonesian plants", Korean Journal of Food Sciences and Technology, 32(4), pp. 949-958, 2000.
  4. G. Kuttan, D. M. Vasudevana, R. Kuttan. "Effect of a Preparation from Viscum album on Tumor Development in vitro and in Mice", Journal of Ethnopharmacology, 29(1), pp. 35-41, 1990. DOI: http://dx.doi.org/10.1016/0378-8741(90)90095-B
  5. U. R. Hengge, B. Benninghoff, T. Ruzicka, M. Goos. "Topical Immunomodulators Progress Towards Treating Inflammation, Infection, and Cancer", Lancet Infectious. Disease, 1(3), pp. 189-198, 2001. DOI: http://dx.doi.org/10.1016/S1473-3099(01)00095-0
  6. B. J. An, C. E. Lee, J. H. Son, et al. "Antioxidant, Anticancer and Tyrosinase Inhibition Activities of Extracts from Rhododendron mucronulatum T", Journal of Korean Society for Applied Biological Chemistry, 48(3), pp. 280-284, 2005.
  7. E. J. Roh, B. K. Kim, D. S. Kim. 'Antioxidative Activity and Antiaging Effects of Tetrapanax papyriferum Extract.", Journal of Korean Oil Chemists Society, 28(2), pp. 219-224, 2011.
  8. J. A. Lee, J. Y. Kim, W. J. Yoon, et al. "Biological Activities of Oenothera laciniata Extracts (Onagraceae, Myrtales)", Korean Journal of Food Sciences and Technology, 38(6), pp. 810-815, 2006.
  9. M. S. Ko. "Chemical Components in Stalks and Leaves of Sasa borealis Makino and Antioxidative and Antimicrobial Activities of Extracts", Korean Journal of Food Preservation, 15(1), pp. 125-132, 2008.
  10. J. W. Nho, I. G. Hwang, E. M. Joung, et al. "Biological Activities of Magnolia denudata Desr. Flower Extracts", .Journal of Korean Society for Food Science and. Nutrition, 38(11), pp. 1478-1484, 2009. DOI: http://dx.doi.org/10.3746/jkfn.2009.38.11.1478
  11. H. J. Ban, M. Y. Kim, K. S. Ko. "A Study on the Cosmeceutical Ingredient of the Methanol Extract of Acanthopanax sessiliflorus Seeman", Journal of Korean Society for Beauty, Culture and Arts, 2(2), pp. 36-42, 2013.
  12. J. S. Yoo, K. K. Lee, M. K. Kim. "Biological Activities of the Hot Water and Ethanol Extracts from Opuntia humifus," Journal of Applied Biological Chemistry, 55(2), pp. 117-121, 2012. DOI: http://dx.doi.org/10.3839/jabc.2011.068
  13. J. Y. Lee. Coloured Korean mushroom. Academy Book Co. Seoul, Korea. p.241, 1993.
  14. L. Mau, H. C. Lin, C. C. Chen. "Non-volatile Components of Several Medicinal Mushroom", Food Research International, 60: pp. 763-766, 1997.
  15. P. D. Critten, and N. Proter. "Lichen forming Fungi: Potential Sources of Novel Metabolites", Trends Biotechnology, 20, pp. 311-315, 1991.
  16. B. W. Lee, M. S. Lee, K. M. Park, et al. "Anticancer Activities of the Extract from the Mycellia of Corious versicolor", Korean Journal of Applied Microbiology and Biotechnology, 20, pp. 311-315, 1992.
  17. Y. Dong. C. Y. Kwan, Z. N. Chen, M. M. Yang. "Antitumor Effect of a Refined Polysaccharide Peptide Fraction isolated from Corious vercicolor in vitro and in vivo studies", Research. Communications in Molecular Pathology and Pharmacology, 92, pp. 140-148, 1996.
  18. K. W. Tsang, C. L. Lam, W. K. Lam. "Corious vercicolor Polysaccharide Peptide Slows Progession of Advanced non-small Cell Lung Cancer", Respiratory Medicine, 97, pp. 618-624, 2003. DOI: http://dx.doi.org/10.1053/rmed.2003.1490
  19. E. K. Park, B. K. Kim. "Antineoplastic Components of Mushroom-antineoplastic Activities of PS-K, a protein-bond Polysaccharide of Coriolus versiccolor (Fr.) quel", Korean Journal of Mycology, 5(2), pp. 25-30, 1997.
  20. S. J. Lee, S. H. Moon, T. Kim, et al. "Anticancer and Antioxidant Activities of Corious versicolor Culture Extracts Cultivated in the Citrus Extracts", Korean Journal of Microbiology and Biotechnology, 31(4), pp. 362-367, 2003.
  21. J. B. Koh. "Effects of Liquid Culture of Corious versicolor on Lipid Metabolism and Enzyme Activities in Rats Fed Cholesterol Diet", Journal of Life Sciences, 15(5), pp. 790-795, 2005. DOI: http://dx.doi.org/10.5352/JLS.2005.15.5.790
  22. K. R. Park, W. J. Lee, M. G. Cho, et al. "Effects of the Extracts from Gyrophora esculenta and Coriolus versicolor judae Mycelia on the Growth of Intestinal Bacteria", Journal of Korean Society for Food Science and Nutrition, 39, pp. 820-825, 2010. DOI: http://dx.doi.org/10.3746/jkfn.2010.39.6.820
  23. V. L. Singleton, R. Orthofer, R. M. Lamuela-Raventos. "Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-ciocalteu reagent", Methods in Enzymology, Elsevier, 1999.
  24. S. K. Lee, S. Y. Park, I. C. Hwang & H. Kang. "Antioxidant and Anti-inflammatory Activities of Ethanol Extracts from Fagopyrum tataricum", Journal of. Naturopathy, 5(1&2), pp. 9-14, 2016.
  25. Korean Society of Food and Nutrition.. Handbook of Food and Nutrition Experiments.. Seoul, Hyoil Publisher, 2000.
  26. A. R. Kim, J. E. Kim, S. N. Park. "Antioxidative Activity and Component Analysis of Phellinus lintes Extracts", Journal of the Society of Cosmetic Scientists of Korea, 37(4), pp. 309-318, 2011.
  27. S. W. Jung, N. K. Lee, S. J. Kim, D. Han. "Screening of Tyrosinase Inhibitor from Plants", Korean Journal of Food Science and Technology, 27(6), pp. 891-896, 1995.
  28. A. E. K.. James, D. W. Timothy, L. Golden. "Inhibition of Human Leukocyte and Porcin Pancreatic Elastase by Homologes of Bovine Pancreatic Tyrosine Inhibitors", Biochemistry, 35, pp. 9090-9096, 1996. DOI: http://dx.doi.org/10.1021/bi953013b
  29. E. Wunsch, H. G. Heindrich. "Zur Quantitative Bestimmung der Collagenase", Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 333, pp. 149-151, 1963. DOI: http://dx.doi.org/10.1515/bchm2.1963.333.1.149
  30. K. H. Lee, H. J. Kwon, S. S. Chun, et al. "Biological Activities of Extracts from Phellinus linteus", Journal of Korean Society for Applied Biological Chemistry, 49(4), pp. 298-303, 2006.
  31. H. S. Yoon, J. S. Choi, K. S. Kim, et al. "Analysis of Physiological Activities of Wild Flammulina velutipes", Journal of Mushroom Society for Production, 8(1), pp. 22-26. 2010.
  32. J. H. Song, H. S. Lee, J. K. Hwang, et al. "Physiological Activities of Phellinus ribis Extracts", Korean Journal of Food Science and Technology, 35(4), pp. 690-695, 2003.
  33. M. K. Kim, K K. Lee, A. R. Jang. "A Study on Cosmetic Physiological Activities of Sasa borealis Root Extracts", Journal of Korean Society for Cosmetics, 16(4), pp. 1017-1027, 2011.
  34. Y. H. Park, S. K. Chang. "Screening of Inhibitory Effect of Edible Mushrooms on Tyrosinase and Isolation of Active Component", Journal of Food Hygiene and Safety, 12(3), pp. 195-199, 1997.