Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Analysis of low molecular organic compounds produced during the spoilage of dairy cattle compound feed

착유우용 배합사료의 부패과정 중 발생하는 저분자 유기화합물의 분석

  • Kim, Yong Tak (Department of Medicinal Biotechnology, Dong-A University) ;
  • Yi, Kwon Jung (Department of Animal Science and Technology, Konkuk University) ;
  • Kim, Gyeom-Heon (Department of Animal Science and Technology, Konkuk University) ;
  • Kim, Dong Woon (National Institute of Animal Science, RDA) ;
  • Kim, Soo Ki (Department of Animal Science and Technology, Konkuk University) ;
  • Moon, Hyung-In (Department of Medicinal Biotechnology, Dong-A University)
  • 김용탁 (동아대학교 의약생명공학과) ;
  • 이권정 (건국대학교 동물자원학과) ;
  • 김겸헌 (건국대학교 동물자원학과) ;
  • 김동운 (축산과학원) ;
  • 김수기 (건국대학교 동물자원학과) ;
  • 문형인 (동아대학교 의약생명공학과)
  • Received : 2016.08.30
  • Accepted : 2016.09.27
  • Published : 2016.09.30
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Abstract

In this study, we analyzed for the changes of low organic compounds during 4 weeks incubation though inoculation of harmful microorganisms on commercial feed. Two percent of overnight cultures of Exiguobacterium acetylicum, Acinetobacter calcoaceticus, Aspergillus flavus and Fusarium graminearum were inoculated in feed, respectively. After adjusting moisture level to 50% for the promotion of feed spoilage, pH was decreased to 4.58~5.03 and microorganism was ranged to $6{\sim}10log_{10}CFU/g$. The compounds were compare between aflatoxin G1 producing feeds and aflatoxin G1 non-producing feeds. Aflatoxins G1 were detected by the immunoaffinity column clean-up method with HPLC-FLD, and were confirmed in samples incoulated by Aspergillus flavus and Acinetobacter calcoaceticus. Koiganal II, cyclohexanol and butadien-one were detected from samples (the non-sterilized inoculated feed) by Aspergillus flavus and Acinetobacter calcoaceticus. Respectively as aflatoxin G1 pre-detected substance, Koiganal II, cyclohexanol and butadien-one may be useful substance for the pre-detection of aflatoxin G1.

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  1. 배합사료의 자연부패과정 중 발생하는 저분자 유기화합물의 동정 및 분석 vol.40, pp.4, 2016, https://doi.org/10.7853/kjvs.2017.40.4.259