Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Dietary effect of green tea extract on hydration improvement and metabolism of free amino acid generation in epidermis of UV-irradiated hairless mice

자외선 조사와 병행된 녹차추출물 식이공급이 무모생쥐의 표피보습 개선 및 유리아미노산 생성 관련 대사에 미치는 영향

  • Choi, Sumin (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Shin, Jihye (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Lee, Bomin (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Cho, Yunhi (Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University)
  • 최수민 (경희대학교 동서의학대학원 의학영양학과) ;
  • 신지혜 (경희대학교 동서의학대학원 의학영양학과) ;
  • 이보민 (경희대학교 동서의학대학원 의학영양학과) ;
  • 조윤희 (경희대학교 동서의학대학원 의학영양학과)
  • Received : 2016.06.10
  • Accepted : 2016.09.05
  • Published : 2016.10.31
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Abstract

Purpose: Ultraviolet (UV) irradiation decreases epidermal hydration, which is maintained by reduction of natural moisturizing factors (NMFs). Among various NMFs, free amino acids (AA) are major constituents generated by filaggrin degradation. This experiment was conducted to determine whether or not dietary supplementation of green tea extract (GTE) in UV-irradiated mice can improve epidermal levels of hydration, filaggrin, free AAs, and peptidylarginine deiminase-3 (PAD3) expression (an enzyme involved in filaggrin degradation). Methods: Hairless mice were fed a diet of 1% GTE for 10 weeks in parallel with UV irradiation (group UV+1%GTE). As controls, hairless mice were fed a control diet in parallel with (group UV+) or without (group UV-) UV irradiation. Results: In group UV+, epidermal levels of hydration and filaggrin were lower than those in group UV-; these levels increased in group UV+1% GTE to levels similar to group UV-. Epidermal levels of PAD3 and major AAs of NMF, alanine, glycine and serine were similar in groups UV- and UV+, whereas these levels highly increased in group UV+1% GTE. Conclusion: Dietary GTE improves epidermal hydration by filaggrin generation and degradation into AAs.

본 연구에서는 자외선 조사를 병행하여 녹차 추출물의 식이공급을 10주간 공급한 무모생쥐 (UV+1%GTE군)의 표피 보습과 filaggrin 의 대사물질 및 관련 효소인 PAD3의 발현 및 함량 변화를 정상대조군 (UV-군) 및 자외선 조사군 (UV+군)과 비교 분석하였으며 결과는 다음과 같다. UV+군의 피부 보습은 UV-군보다 유의적으로 낮은 반면 UV+1%GTE군 의 피부 보습은 UV+군에 비해 유의적으로 높았고, UV-군에는 미치지 못하였다. UV+군의 profilaggrin 함량은 UV-군과 유의적인 차이가 없었으나, filaggrin의 함량은 UV-군에 비해 유의적으로 낮았다. UV+1%GTE군 에서 profilaggrin은 UV- 및 UV+에비해 현저히 높았고, filaggrin은 UV-군과 유사한 수준으로 높았다. UV+군의 PAD3 효소 발현은 UV-군에 비해 유의적으로 낮았다. UV+1%GTE군은 UV+군에 비해 유의적으로 높았으며, UV-군에 비해 높았다. 자연보습인자의 주요 아미노산인 serine 및 glycine, alanine의 함량은 UV-군과 UV+군간 유사하였으나 UV+1%GTE군에서는 UV- 및 UV+군에 비해 현저히 높았다. UV-군과 비교하여 UV+군의 피부 보습함량이 낮게 측정된 것에 비해 유리아미노산 함량에는 변화가 없어 자외선에 따른 피부 보습에 관여되는 또 다른 메커니즘인 표피 지질함량 분석과 함께 녹차추출물의 효과에 대한 연구가 추후 이루어질 필요성이 있다. 그러나, 결론적으로 자외선 조사와 병행된 녹차 추출물 식이 공급은 (pro)filaggrin의 함량 및 PAD3의 발현이 높게 나타남에 의한 표피 유리아미노산의 함량이 높아 궁극적으로 자외선 조사에 의해 야기된 피부건조를 완화시켰다.

Keywords

References

  1. Rawlings AV, Harding CR. Moisturization and skin barrier function. Dermatol Ther 2004; 17 Suppl 1: 43-48. https://doi.org/10.1111/j.1396-0296.2004.04S1005.x
  2. Baroni A, Buommino E, De Gregorio V, Ruocco E, Ruocco V, Wolf R. Structure and function of the epidermis related to barrier properties. Clin Dermatol 2012; 30(3): 257-262. https://doi.org/10.1016/j.clindermatol.2011.08.007
  3. Verdier-Sevrain S, Bont F. Skin hydration: a review on its molecular mechanisms. J Cosmet Dermatol 2007; 6(2): 75-82. https://doi.org/10.1111/j.1473-2165.2007.00300.x
  4. Sandilands A, Sutherland C, Irvine AD, McLean WH. Filaggrin in the frontline: role in skin barrier function and disease. J Cell Sci 2009; 122(Pt 9): 1285-1294. https://doi.org/10.1242/jcs.033969
  5. Nachat R, Méchin MC, Takahara H, Chavanas S, Charveron M, Serre G, Simon M. Peptidylarginine deiminase isoforms 1-3 are expressed in the epidermis and involved in the deimination of K1 and filaggrin. J Invest Dermatol 2005; 124(2): 384-393. https://doi.org/10.1111/j.0022-202X.2004.23568.x
  6. Gilchrest BA. Skin aging and photoaging: an overview. J Am Acad Dermatol 1989; 21(3 Pt 2): 610-613. https://doi.org/10.1016/S0190-9622(89)70227-9
  7. Chung JH. Photoaging in Asians. Photodermatol Photoimmunol Photomed 2003; 19(3): 109-121. https://doi.org/10.1034/j.1600-0781.2003.00027.x
  8. Tagami H. Functional characteristics of the stratum corneum in photoaged skin in comparison with those found in intrinsic aging. Arch Dermatol Res 2008; 300 Suppl 1: S1-S6.
  9. Gruber JV, Holtz R. Examining communication between ultraviolet (UV)-damaged cutaneous nerve cells and epidermal keratinocytes in vitro. Toxicol Ind Health 2009; 25(4-5): 225-230. https://doi.org/10.1177/0748233709103412
  10. Hwang E, Sun ZW, Lee TH, Shin HS, Park SY, Lee DG, Cho BG, Sohn H, Kwon OW, Kim SY, Yi TH. Enzyme-processed Korean Red Ginseng extracts protects against skin damage induced by UVB irradiation in hairless mice. J Ginseng Res 2013; 37(4): 425-434. https://doi.org/10.5142/jgr.2013.37.425
  11. Kim J, Lee Y, Cho Y. Effects of dietary royal jelly on epidermal generation of ceramides from acidic sphingomyelin and glucosylceramides in UV-irradiated hairless mice. Curr Top Nutraceutical Res 2012; 10(3/4): 151-164.
  12. Khan SG, Katiyar SK, Agarwal R, Mukhtar H. Enhancement of antioxidant and phase II enzymes by oral feeding of green tea polyphenols in drinking water to SKH-1 hairless mice: possible role in cancer chemoprevention. Cancer Res 1992; 52(14): 4050-4052.
  13. Min J, Lee Y, Han SM, Choi Y. Dietary effect of royal jelly supplementation on epidermal levels of hydration, filaggrins, free amino acids and the related enzyme expression in UV irradiated hairless mice. Korean J Nutr 2013; 46(2): 109-118. https://doi.org/10.4163/kjn.2013.46.2.109
  14. Katiyar SK, Elmets CA. Green tea polyphenolic antioxidants and skin photoprotection (Review). Int J Oncol 2001; 18(6): 1307-1313.
  15. Jackson JK, Zhao J, Wong W, Burt HM. The inhibition of collagenase induced degradation of collagen by the galloyl-containing polyphenols tannic acid, epigallocatechin gallate and epicatechin gallate. J Mater Sci Mater Med 2010; 21(5): 1435-1443. https://doi.org/10.1007/s10856-010-4019-3
  16. Hsu S, Bollag WB, Lewis J, Huang Q, Singh B, Sharawy M, Yamamoto T, Schuster G. Green tea polyphenols induce differentiation and proliferation in epidermal keratinocytes. J Pharmacol Exp Ther 2003; 306(1): 29-34. https://doi.org/10.1124/jpet.103.049734
  17. Kiss I, Chen S, Tramposch KM. The effect of high and low ultraviolet-B dose exposure on the degree of hairless mouse skin wrinkling. Photochem Photobiol 1991; 53(1): 109-112. https://doi.org/10.1111/j.1751-1097.1991.tb08474.x
  18. Takami S, Imai T, Hasumura M, Cho YM, Onose J, Hirose M. Evaluation of toxicity of green tea catechins with 90-day dietary administration to F344 rats. Food Chem Toxicol 2008; 46(6): 2224-2229. https://doi.org/10.1016/j.fct.2008.02.023
  19. Lee B, Kim J, Hwang J, Cho Y. Dietary effect of green tea extract on epidermal levels of skin pH related factors, lactate dehydrogenase protein expression and activity in UV-irradiated hairless mice. J Nutr Health 2016; 49(2): 63-71. https://doi.org/10.4163/jnh.2016.49.2.63
  20. Ginger RS, Blachford S, Rowland J, Rowson M, Harding CR. Filaggrin repeat number polymorphism is associated with a dry skin phenotype. Arch Dermatol Res 2005; 297(6): 235-241. https://doi.org/10.1007/s00403-005-0590-8
  21. Dale BA, Resing KA, Lonsdale-Eccles JD. Filaggrin: a keratin filament associated protein. Ann N Y Acad Sci 1985; 455: 330-342. https://doi.org/10.1111/j.1749-6632.1985.tb50420.x
  22. Kanno T, Kawada A, Yamanouchi J, Yosida-Noro C, Yoshiki A, Shiraiwa M, Kusakabe M, Manabe M, Tezuka T, Takahara H. Human peptidylarginine deiminase type III: molecular cloning and nucleotide sequence of the cDNA, properties of the recombinant enzyme, and immunohistochemical localization in human skin. J Invest Dermatol 2000; 115(5): 813-823. https://doi.org/10.1046/j.1523-1747.2000.00131.x
  23. Steinert PM, Cantieri JS, Teller DC, Lonsdale-Eccles JD, Dale BA. Characterization of a class of cationic proteins that specifically interact with intermediate filaments. Proc Natl Acad Sci U S A 1981; 78(7): 4097-4101. https://doi.org/10.1073/pnas.78.7.4097
  24. Visscher M, Robinson M, Wickett R. Stratum corneum free amino acids following barrier perturbation and repair. Int J Cosmet Sci 2011; 33(1): 80-89. https://doi.org/10.1111/j.1468-2494.2010.00592.x
  25. Yamada Y, Obayashi M, Ishikawa T, Kiso Y, Ono Y, Yamashita K. Dietary tocotrienol reduces UVB-induced skin damage and sesamin enhances tocotrienol effects in hairless mice. J Nutr Sci Vitaminol (Tokyo) 2008; 54(2): 117-123. https://doi.org/10.3177/jnsv.54.117
  26. Scott IR, Harding CR, Barrett JG. Histidine-rich protein of the keratohyalin granules. Source of the free amino acids, urocanic acid and pyrrolidone carboxylic acid in the stratum corneum. Biochim Biophys Acta 1982; 719(1): 110-117. https://doi.org/10.1016/0304-4165(82)90314-2
  27. Kam E, Resing KA, Lim SK, Dale BA. Identification of rat epidermal profilaggrin phosphatase as a member of the protein phosphatase 2A family. J Cell Sci 1993; 106(Pt 1): 219-226.
  28. Pearton DJ, Nirunsuksiri W, Rehemtulla A, Lewis SP, Presland RB, Dale BA. Proprotein convertase expression and localization in epidermis: evidence for multiple roles and substrates. Exp Dermatol 2001; 10(3): 193-203. https://doi.org/10.1034/j.1600-0625.2001.010003193.x
  29. Jiang SJ, Chu AW, Lu ZF, Pan MH, Che DF, Zhou XJ. Ultraviolet B-induced alterations of the skin barrier and epidermal calcium gradient. Exp Dermatol 2007; 16(12): 985-992. https://doi.org/10.1111/j.1600-0625.2007.00619.x
  30. Jang SI, Steinert PM, Markova NG. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J Biol Chem 1996; 271(39): 24105-24114. https://doi.org/10.1074/jbc.271.39.24105
  31. Balasubramanian S, Efimova T, Eckert RL. Green tea polyphenol stimulates a Ras, MEKK1, MEK3, and p38 cascade to increase activator protein 1 factor-dependent involucrin gene expression in normal human keratinocytes. J Biol Chem 2002; 277(3): 1828-1836. https://doi.org/10.1074/jbc.M110376200
  32. Hoste E, Kemperman P, Devos M, Denecker G, Kezic S, Yau N, Gilbert B, Lippens S, De Groote P, Roelandt R, Van Damme P, Gevaert K, Presland RB, Takahara H, Puppels G, Caspers P, Vandenabeele P, Declercq W. Caspase-14 is required for filaggrin degradation to natural moisturizing factors in the skin. J Invest Dermatol 2011; 131(11): 2233-2241. https://doi.org/10.1038/jid.2011.153
  33. Kim Y, Han SM, Cho Y. The dietary effect of royal jelly supplementation on epidermal levels of filaggrin and free amino acids during menopause in rats. J Korean Soc Food Sci Nutr 2013; 42(3): 389-396. https://doi.org/10.3746/jkfn.2013.42.3.389
  34. Hsu S, Yamamoto T, Borke J, Walsh DS, Singh B, Rao S, Takaaki K, Nah-Do N, Lapp C, Lapp D, Foster E, Bollag WB, Lewis J, Wataha J, Osaki T, Schuster G. Green tea polyphenol-induced epidermal keratinocyte differentiation is associated with coordinated expression of p57/KIP2 and caspase 14. J Pharmacol Exp Ther 2005; 312(3): 884-890. https://doi.org/10.1124/jpet.104.076075
  35. Koyama J, Horii I, Kawasaki K, Nakayama Y, Morikawa Y, Mitsui T, Kumagai H. Free amino acids of stratum corneum as a biochemical marker to evaluate dry skin. J Soc Cosmet Chem 1984; 35(4): 183-195.
  36. Tabachnick J, LaBadie JH. Studies on the biochemistry of epidermis. IV. The free amino acids, ammonia, urea, and pyrrolidone carboxylic acid content of conventional and germ-free albino guina pig epidermia. J Invest Dermatol 1970; 54(1): 24-31. https://doi.org/10.1111/1523-1747.ep12551492
  37. Joo KM, Han JY, Son ED, Nam GW, Jeong HJ, Lim KM, Cho JC. Study on the relationship between skin dryness and amino acids in stratum corneum. J Soc Cosmet Sci Korea 2012; 38(1): 75-82. https://doi.org/10.15230/SCSK.2012.38.1.075
  38. Bak H, Hong SP, Jeong SK, Choi EH, Lee SE, Lee SH, Ahn SK. Altered epidermal lipid layers induced by long-term exposure to suberythemal-dose ultraviolet. Int J Dermatol 2011; 50(7): 832-837. https://doi.org/10.1111/j.1365-4632.2010.04789.x
  39. Kim H, Oh I, Park KH, Kim NM, Do JH, Cho Y. Stimulatory effect of dietary red ginseng on epidermal hydration and ceramide levels in ultraviolet-irradiated hairless mice. J Med Food 2009; 12(4): 746-754. https://doi.org/10.1089/jmf.2008.1185
  40. Kezic S, Kammeyer A, Calkoen F, Fluhr JW, Bos JD. Natural moisturizing factor components in the stratum corneum as biomarkers of filaggrin genotype: evaluation of minimally invasive methods. Br J Dermatol 2009; 161(5): 1098-1104. https://doi.org/10.1111/j.1365-2133.2009.09342.x
  41. Mark H, Harding CR. Amino acid composition, including key derivatives of eccrine sweat: potential biomarkers of certain atopic skin conditions. Int J Cosmet Sci 2013; 35(2): 163-168. https://doi.org/10.1111/ics.12019

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