Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Microbial Monitoring and Exploring Ways to Prevent or Minimize Microbial Contamination at the Production and Distribution Stages of Fresh Strawberries

신선한 딸기의 생산 및 유통 단계에서의 미생물 모니터링 및 미생물 오염 방지 또는 저감화 방법 모색

  • Kim, Sol-A (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Lee, Jeong-Eun (Division of Applied Life Science, Graduate School, Gyeongsang National University) ;
  • Kim, Go-Un (Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) ;
  • Kim, Soo-Hwan (Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University) ;
  • Shim, Won-Bo (Department of Agricultural Chemistry and Food Science & Technology, Gyeongsang National University)
  • 김솔아 (경상대학교 응용생명과학부) ;
  • 이정은 (경상대학교 응용생명과학부) ;
  • 김고운 (경상대학교 농화학식품공학과) ;
  • 김수환 (경상대학교 농화학식품공학과) ;
  • 심원보 (경상대학교 농화학식품공학과)
  • Received : 2017.10.10
  • Accepted : 2017.11.12
  • Published : 2017.12.30
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Abstract

This study investigated to determine the microbial contamination levels of strawberries at harvest and distribution stages and to suggest a control measure for reducing the microbial contamination of strawberries by replacing worker's gloves used at harvest and distribution stages. According to the monitoring results, the contamination levels of total aerobic bacteria (TAB) were in the order of soil ($7.12{\pm}0.61{\log}_{10}CFU/g$), gloves ($6.06{\pm}1.80{\log}_{10}CFU/cm^2$), strawberry ($3.28{\times}0.98{\log}_{10}CFU/g$), and water ($3.08{\pm}0.55{\log}_{10}CFU/g$) at harvest stage. TAB of strawberry at was harvest stage reduced from $3.28{\pm}0.98{\log}_{10}CFU/g$ to $1.85{\pm}0.21{\log}_{10}CFU/g$ and $2.6{\pm}0.30{\log}_{10}CFU/g$ at cold and room temperature storage, respectively. By the replacement of worker's gloves and distribution temperature, TAB levels of the strawberries were significantly reduced when compared to those of the strawberries treated without replacement of worker's gloves and distributed at room temperature. For reusing the replaced gloves, washing with a commercial disinfectant, clorox, was effective to reduce microorganisms contaminated on the worker's gloves. These results demonstrated that appropriate replacement of gloves at the harvest and distribution stages is an effective method for reducing microbial contamination of fresh strawberries.

이 연구는 수확 및 유통 단계에서 딸기의 미생물 오염 수준을 확인하고 수확 단계에서 사용 된 작업자 장갑을 교체하고 유통 온도를 제어함으로써 딸기에 대한 미생물 오염을 줄일 수 있는 방법을 확인하고자 하였다. 모니터링 결과 수확 단계에서 일반세균의 오염 수준은 토양 평균 $7.12{\pm}0.61{\log}_{10}CFU/g$, 장갑 평균 $6.06{\pm}1.80{\log}_{10}CFU/g$, 딸기 평균 $3.28{\pm}0.98{\log}_{10}CFU/g$, 용수 평균 $3.08{\pm}0.55{\log}_{10}CFU/g$ 순으로 높았다. 수확 단계의 딸기에서 평균적으로 $3.3{\log}_{10}CFU/g$의 일반세균이 검출되었으며, 저온 및 상온에서 각각 분포 된 딸기에서 $1.85{\pm}0.21{\log}_{10}CFU/g$ 및 $2.6{\pm}0.21{\log}_{10}CFU/g$수준의 일반세균 수가 확인되어 수확단계보다는 감소된 결과를 확인할 수 있었다. 작업자의 장갑 교체 및 냉장 유통 조건에 따라 딸기의 일반세균은 작업자의 장갑을 교체하지 않고 처리 한 딸기와 실온에서 유통 한 딸기의 일반세균 수준보다 유의적으로 낮게 확인되었다. 교체 된 장갑을 재활용하기 위해 시판 소독제 clorox로 세척하는 것과 삶아서 세척하는 것은 사용 후 작업자의 장갑에서 오염 된 미생물을 제어하기 위한 효과적인 세척 방법임이 확인되었다. 이 결과는 수확 단계에서 장갑을 주기적으로 교체하고 현장에서 쉽게 수행 할 수 있는 냉장 유통 조건이 딸기의 미생물 오염을 감소시키는데 효과적이라는 것을 확인할 수 있었다.

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References

  1. Kim, J.S., Shim, W.B., Kim, J.H., Kim, S.R., and Chung D.H.: Sanitary microbial distribution at the tomato farms in western Gyeongnam. Korean J. Environ. Health, 32, 77-88 (2006).
  2. Kim, J.W., and Kim, S.H.: Establishment of washing conditions for salad to reduce the microbial hazard. Korean J. Food and Cook. Sci., 21, 703-708 (2005).
  3. Yu, K.M., Newman, C., Archbold, D.D. and Hamilton- Kemp, T.R.: Survival of Escherichia coli O157: H7 on strawberry fruit and reduction of the pathogen population by chemical agents, J. Food Prot., 64, 1334-1340 (2001). https://doi.org/10.4315/0362-028X-64.9.1334
  4. Food and Drug Administration: Hazard analysis and critical control point (HACCP); procedures for the safe and sanitary processing and importing of juice. Fed. Regist., 63, 20450- 20486 (2001).
  5. Won, J.S., Kim, M.H., Han, G.J., Noh, B.S. and Min. S.C.: Preservation of strawberry juice by dynamic high-pressure processing. Korean J. Food Sci. Technol., 47, 480-485 (2015). https://doi.org/10.9721/KJFST.2015.47.4.480
  6. Shim, W.B., Kim, K.Y., Yoon, Y.H., Kim, J.E., Shim, S.I, Kim, Y.S. and Chung D.H.: Microbiological hazard analysis for strawberry farms at the harvest stage to establish Good Agricultural Practices (GAP) model based on principle of HACCP, Korean J. Food Sci. Technol., 45,104-110 (2013). https://doi.org/10.9721/KJFST.2013.45.1.104
  7. Park, S.S., Sung, J.M., Jeong, J.W., Park, K.J. and Lim, J.H.: Efficacy of electrolyzed water and aqueous chlorine dioxide for reducing pathogenic microorganism on chinese cabbage, Korean J. Food Sci. Technol., 44, 240-246 (2012). https://doi.org/10.9721/KJFST.2012.44.2.240
  8. Choi, J.K., Shin, I.S., Kim, D.U., and Kim, H.Y.: Control of microorganisms in school refectories and kitchens using ozone water and ozone gas, Korean J. Food Sci. Technol., 47, 586-592 (2015). https://doi.org/10.9721/KJFST.2015.47.5.586
  9. Lee, J.S., Kim, J.G. and Park, S.h.: Effects of chlorine wash on the quality and microbial population of tah Tsai Chinese Cabbage (Brassica campestris var. narinosa) microgreen. Korean J. Hort. Sci. Technol., 27, 625-630 (2009).
  10. Kim, K.M.: Color changes in fresh-cut fruit and vegetables and its pretreatment processing. J. Korea Soc. Packag. Sci. Technol., 14, 23-29 (2008).
  11. Kim, D.M.: Extension of freshness of minimally processed fruits and vegetables. Korea J. Hort. Sci. Technol., 17, 790- 795 (1999).
  12. Sun, S.H., Kim, S.J., Kim, G.C., Kim, H.R and Yoon, K.S.: Changes in quality characteristics of fresh-cut produce during refrigerated storage. Korean J. Food Sci. Technol., 43, 495- 503 (2011). https://doi.org/10.9721/KJFST.2011.43.4.495
  13. Kim, J.E., Kim, I.H., and Min, S.C.: Microbial decontamination of vegetables and spices using cold plasma treatments, Korean J. Food Sci. Technol., 45, 735-741 (2013) https://doi.org/10.9721/KJFST.2013.45.6.735
  14. Byrene, R.D. JR., and Bishop, J.R.: Evaluation of a dry medium culture plate (3M Petri film AC) for laboratory pasteurized counts. J. Food Prot., 54, 308-309 (1991). https://doi.org/10.4315/0362-028X-54.4.308
  15. Schraft H. and Watterworth L.A.: Enumeration of heterotrophs, fecal coliforms and Escherichia coli in water: comparison of 3MTM PetrifilmTM plates with standard plating procedures, J. Micro. Methods, 60, 335-342 (2005). https://doi.org/10.1016/j.mimet.2004.10.008
  16. Beuchat, L. R., Nail, B. V., Brackett, R.E., and Fox, T. L.: Comparison of the Petrifilm Yeast and Mold culture film method to conventional methods for enumerating Yeasts and Molds in foods. J. Food Prot., 54, 443-447 (1991). https://doi.org/10.4315/0362-028X-54.6.443
  17. Kim, S.R.: Assessment of microbiological hazard for the construction of HACCP in non-pasteurized strawberry juice. MS thesis, Gyeongsang National University, Jinju, Korea (2005).