Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지
DOI QR코드

DOI QR Code

Ameliorating Effects of Cinnamomum loureiroi and Rosa laevigata Extracts Mixture against Trimethyltin-induced Learning and Memory Impairment Model

트리메틸틴 처리로 유도된 기억·학습 능력 손상 모델에 대한 계피와 금앵자 혼합추출물의 개선 효과

  • 최수정 (고려대학교 건강기능식품 연구센터) ;
  • 김초롱 (고려대학교 식품생명공학과) ;
  • 박찬규 (고려대학교 식품생명공학과) ;
  • 김민철 (고려대학교 식품생명공학과) ;
  • 최종헌 ((주)네추럴웨이) ;
  • 신동훈 (고려대학교 식품생명공학과)
  • Received : 2017.09.18
  • Accepted : 2017.12.12
  • Published : 2017.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: A critical features of Alzheimer's disease (AD) is cognitive dysfunction, which partly arises from decreased in acetylcholine levels. AD afftected brains are characterized by extensive oxidative stress, which is thought to be primarily induced by the amyloid beta ($A{\beta}$) peptide. In a previous study, Cinnamomum loureiroi tincture inhibited acetylcholinesterase (AchE) activity. That study identified AChE inhibitor in the C. loureiroi extract. Furthermore, the C. loureiroi extract enhanced memory in a trimethyltin (TMT)-induced model of cognitive dysfunction, as assessed via two behavioral tests. Rosa laevigata extract protected against oxidative stress-induced cytotoxicity. Administrating R. laevigata extracts to mice significantly reversed $A{\beta}$-induced learning and memory impairment, as shown in behavioral tests. Methods and Results: We conducted behavioral to examine the synergistic effects of C. loureiroi and R. laevigata extracts in inhibiting AChE and counteracting TMT-induced learning and memory losses. We also performed biochemical assays. The biochemical results showed a relationship between increased oxidative stress and cholinergic neurons damage in TMT-treated mice. Conclusions: A diet containing C. loureiroi and R. laevigata extracts ameliorated learning and memory impairments in the Y-maze and passive avoidance tests, and exerted synergistic inhibitory effect against AChE and lipid peroxidation.

Keywords

References

  1. Berchtold NC and Cotman CW. (1998). Evolution in the conceptualization of dementia and Alzheimer's disease: Greco-roman period to the 1960s. Neurobiology of Aging. 19:173-189. https://doi.org/10.1016/S0197-4580(98)00052-9
  2. Butterfield DA, Reed T, Newman SF and Sultana R. (2007). Roles of amyloid ${\beta}$-peptide-associated oxidative stress and brain protein modifications in the pathogenesis of Alzheimer's disease and mild cognitive impairment. Free Radical Biology and Medicine. 43:658-677. https://doi.org/10.1016/j.freeradbiomed.2007.05.037
  3. Choi SJ, Kim JK, Kim HK, Harris K, Kim CJ, Park GG, Park CS and Shin DH. (2013). 2,4-Di-tert-butylphenol from sweet potato protects against oxidative stress in PC12 cells and in mice. Journal of Medicinal Food. 16:977-983. https://doi.org/10.1089/jmf.2012.2739
  4. Choi SJ, Kim MJ, Heo HJ, Kim HK, Hong B, Kim CJ, Kim BG and Shin DH. (2006). Protective effect of Rosa laevigata against amyloid beta peptide-induced oxidative stress. Amyloid: The Journal of Protein Folding Disorders. 13:6-12. https://doi.org/10.1080/13506120500535636
  5. Choi SJ, Kim MJ, Heo HJ, Kim JK, Jun WJ, Kim HK, Kim EK, Kim MO, Cho HY, Hwang HJ, Kim YJ and Shin DH. (2009). Ameliorative effect of 1,2-benzenedicarboxylic acid dinonyl ester against amyloid beta peptide-induced neurotoxicity. Amyloid: The Journal of Protein Folding Disorders. 16:15-24. https://doi.org/10.1080/13506120802676997
  6. Choi SJ, Oh SS, Kim CR, Kwon YK, Suh SH, Kim JK, Park GG, Son SY and Shin DH. (2016). Perilla frutescens extract ameliorates acetylcholinesterase and trimethyltin chloride-induced neurotoxicity. Journal of Medicinal Food. 19:281-289. https://doi.org/10.1089/jmf.2015.3540
  7. Ellman GL, Courtney KD, Andres jr V and Featherstone RM. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology. 7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  8. Geloso MC, Corvino V and Michetti F. (2011). Trimethyltin-induced hippocampal degeneration as a tool to investigate neurodegenerative processes. Neurochemistry International. 58:729-738. https://doi.org/10.1016/j.neuint.2011.03.009
  9. Hestrin S. (1949). The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. Journal of Biological Chemistry. 180:249-261.
  10. Inestrosa NC, Alvarez A, Perez CA, Moreno RD, Vicente M, Linker C, Casanueva OI, Soto C and Garrido J. (1996). Acetylcholinesterase accelerates assembly of amyloid-${\beta}$-peptides into Alzheimer's fibrils: Possible role of the peripheral site of the enzyme. Neuron. 16:881-891. https://doi.org/10.1016/S0896-6273(00)80108-7
  11. Jung MH, Song KS and Seong YH. (2012). Inhibitory effect of Chaenomeles sinensis fruit on amyloid ${\beta}$ protein (25-35)-induced neurotoxicity in cultured neurons and memory impairment in mice. Korean Journal of Medicinal Crop Science. 20:8-15. https://doi.org/10.7783/KJMCS.2012.20.1.008
  12. Kim CR, Choi SJ, Kim JK, Park CK, Gim MC, Kim YJ, Park GG and Shin DH. (2017). 2,4-Bis(1,1-dimethylethyl)phenol from Cinnamomum loureirii improves cognitive deficit, cholinergic dysfunction, and oxidative damage in tmt-treated mice. Biological and Pharmaceutical Bulletin. 40:932-935. https://doi.org/10.1248/bpb.b16-00997
  13. Kim CR, Choi SJ, Kwon YK, Kim JK, Kim YJ, Park GG and Shin DH. (2016). Cinnamomum loureirii extract inhibits acetylcholinesterase activity and ameliorates trimethyltin-induced cognitive dysfunction in mice. Biological and Pharmaceutical Bulletin. 39:1130-1136. https://doi.org/10.1248/bpb.b16-00045
  14. Kim JK, Choi SJ, Bae H, Kim CR, Cho HY, Kim YJ, Lim ST, Kim CJ, Kim HK, Peterson S and Shin DH. (2011). Effects of methoxsalen from poncirus trifoliata on acetylcholinesterase and trimethyltin-induced learning and memory impairment. Bioscience Biotechnology and Biochemistry. 75:1984-1989. https://doi.org/10.1271/bbb.110386
  15. Loullis CC, Dean RL, Lippa AS, Clody DE and Coupet J. (1985). Hippocampal muscarinic receptor loss following trimethyl tin administration. Pharmacology Biochemistry and Behavior. 22:147-151. https://doi.org/10.1016/0091-3057(85)90498-8
  16. McGleenon BM, Dynan KB and Passmore AP. (1999). Acetylcholinesterase inhibitors in Alzheimer's disease. British Journal of Clinical Pharmacology. 48:471-480.
  17. Nabeshima T and Nitta A. (1994). Memory impairment and neuronal dysfunction induced by ${\beta}$-amyloid protein in rats. The Tohoku Journal of Experimental Medicine. 174:241-249. https://doi.org/10.1620/tjem.174.241
  18. Ohno K, Tsujino A, Brengman JM, Harper CM, Bajzer Z, Udd B, Beyring R, Robb S, Kirkham FJ and Engel AG. (2001). Choline acetyltransferase mutations cause myasthenic syndrome associated with episodic apnea in humans. Proceedings of the National Academy of Sciences of the United States of America. 98:2017-2022. https://doi.org/10.1073/pnas.98.4.2017
  19. Shuto M, Higuchi K, Sugiyama C, Yoneyama M, Kuramoto N, Nagashima R, Kawada K and Ogita K. (2009). Endogenous and exogenous glucocorticoids prevent trimethyltin from causing neuronal degeneration of the mouse brain in vivo: Involvement of oxidative stress pathways. Journal of Pharmacological Sciences. 110:424-436. https://doi.org/10.1254/jphs.09107FP
  20. Smith DG, Cappai R and Barnham KJ. (2007). The redox chemistry of the Alzheimer's disease amyloid ${\beta}$ peptide. Biochimica et Biophysica Acta (BBA)-Biomembranes. 1768:1976-1990. https://doi.org/10.1016/j.bbamem.2007.02.002
  21. Wang X, Cai J, Zhang J, Wang C, Yu A, Chen Y and Zuo Z. (2008). Acute trimethyltin exposure induces oxidative stress response and neuronal apoptosis in Sebastiscus marmoratus. Aquatic Toxicology. 90:58-64. https://doi.org/10.1016/j.aquatox.2008.07.017
  22. Ko Wc and Lee SR. (2015). Effect of immature Citrus sunki peel extract on neuronal cell death. Korean Journal of Medicinal Crop Science. 23:144-149. https://doi.org/10.7783/KJMCS.2015.23.2.144