The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Protective Effect of PineXol® against Amyloid-β-induced Cell Death

아밀로이드 베타로 유도된 신경세포 사멸에 대한 PineXol®의 보호효과

  • 한경훈 (서울의료원 의학연구소) ;
  • 이승희 (서울의료원 의학연구소) ;
  • 박광성 (서울의료원 의학연구소) ;
  • 송관영 (서울의료원 신경외과) ;
  • 김정희 (서울의료원 신경외과) ;
  • 박은국 (아주대학교 의과대학 의학유전학과) ;
  • 한성희 (고려대학교 보건과학대학 BK21Plus 보건과학사업단)
  • Received : 2017.09.15
  • Accepted : 2017.11.07
  • Published : 2017.12.31
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Abstract

$Amyloid-{\beta}$ protein ($A{\beta}$) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of $PineXol^{(R)}$ on $A{\beta}_{25-35}$ induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with $100{\mu}g/mL$ of $PineXol^{(R)}$ for 2 h. The cells were exposed to single dose of $30{\mu}M$ $A{\beta}_{25-35}$ for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both $A{\beta}_{25-35}$ and $PineXol^{(R)}$, compared to the control group. $PineXol^{(R)}$ induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of $A{\beta}_{25-35}$ induced apoptosis. These results suggest that $PineXol^{(R)}$ may be a good candidate for the prevention of Alzheimer's disease(AD).

Keywords

References

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