Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Comparison of Analytical Methods for the Detection of Paralytic Shellfish Toxins (PSTs)

마비성패류독소 검출을 위한 분석법 비교

  • Lee, Ka Jeong (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Kwon, Soon Jae (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Jung, Yeoun Joong (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Son, Kwang Tae (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science) ;
  • Ha, Kwang Soo (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Mok, Jong Soo (Food Safety and Processing Research Division, National Institute of Fisheries Science) ;
  • Kim, Ji Hoe (Research and Development Planning Division, National Institute of Fisheries Science)
  • 이가정 (국립수산과학원 남동해수산연구소) ;
  • 권순재 (국립수산과학원 남동해수산연구소) ;
  • 정연중 (국립수산과학원 남동해수산연구소) ;
  • 손광태 (국립수산과학원 남동해수산연구소) ;
  • 하광수 (국립수산과학원 식품위생가공과) ;
  • 목종수 (국립수산과학원 식품위생가공과) ;
  • 김지회 (국립수산과학원 연구기획과)
  • Received : 2017.10.31
  • Accepted : 2017.12.08
  • Published : 2017.12.31
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Abstract

Paralytic shellfish toxins (PSTs) are produced by marine dinoflagellate phytoplankton Alexandrium spp. and Gymnodinium spp. These toxins accumulate in filter feeding organisms such as bivalves and the ingestion of contaminated shellfish can cause illness in humans. The mouse bioassay (MBA) has been the preferred PST testing method worldwide for more than 50 years. However, this assay has several disadvantages, such as detection limits, non-toxic-profiles, and the ethical issues of using animals. The aim of this study was to establish an alternative to the MBA method for testing for PSTs. We optimized the analysis conditions of a post-column oxidation-high performance liquid chromatography (PCOX-HPLC) method and the Scotia Rapid Test Kit, and then compared the accuracy of these methods to the MBA method. The results demonstrated a strong correlation between the PCOX-HPLC method and the MBA, although the PCOX-HPLC method required expensive equipment and standard material, and was time consuming. The Scotia Rapid Test Kit promises to be a useful tool, as it provided rapid and qualitative results, although the method sometimes gave a false positive result that could not be explained by toxin profiles.

Keywords

References

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