Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effects of Extended Storage of Chlorhexidine Gluconate and Benzalkonium Chloride Solutions on the Viability of Burkholderia cenocepacia

  • Ahn, Youngbeom (Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration) ;
  • Kim, Jeong Myeong (Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration) ;
  • Lee, Yong-Jin (Department of Biological Sciences, Albany State University) ;
  • LiPuma, John J. (Department of Pediatrics & Communicable Diseases, University of Michigan) ;
  • Hussong, David (ValSource, LLC.) ;
  • Marasa, Bernard S. (Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration) ;
  • Cerniglia, Carl E. (Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration)
  • Received : 2017.06.14
  • Accepted : 2017.10.10
  • Published : 2017.12.28
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Abstract

Chlorhexidine gluconate (CHX) and benzalkonium chloride (BZK) formulations are frequently used as antiseptics in healthcare and consumer products. Burkholderia cepacia complex (BCC) contamination of pharmaceutical products could be due to the use of contaminated water in the manufacturing process, over-diluted antiseptic solutions in the product, and the use of outdated products, which in turn reduces the antimicrobial activity of CHX and BZK. To establish a "afe use" period following opening containers of CHX and BZK, we measured the antimicrobial effects of CHX ($2-10{\mu}g/ml$) and BZK ($10-50{\mu}g/ml$) at sublethal concentrations on six strains of Burkholderia cenocepacia using chemical and microbiological assays. CHX (2, 4, and $10{\mu}g/ml$) and BZK (10, 20, and $50{\mu}g/ml$) stored for 42 days at $23^{\circ}C$ showed almost the same concentration and toxicity compared with freshly prepared CHX and BZK on B. cenocepacia strains. When $5{\mu}g/ml$ CHX and $20{\mu}g/ml$ BZK were spiked to six B. cenocepacia strains with different inoculum sizes ($10^0-10^5CFU/ml$), their toxic effects were not changed for 28 days. B. cenocepacia strains in diluted CHX and BZK were detectable at concentrations up to $10^2CFU/ml$ after incubation for 28 days at $23^{\circ}C$. Although abiotic and biotic changes in the toxicity of both antiseptics were not observed, our results indicate that B. cenocepacia strains could remain viable in CHX and BZK for 28 days, which in turn, indicates the importance of control measures to monitor BCC contamination in pharmaceutical products.

Keywords

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