Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
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Probiotic Properties of Lactic Acid Bacteria isolated from Feces and Kimchi

베트남인 분변 및 김치로부터 분리된 유산균의 프로바이오틱스 기능성 연구

  • Received : 2017.12.06
  • Accepted : 2017.12.20
  • Published : 2017.12.31
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Abstract

The purpose of this study was to investigate the probiotic properties and antioxidant capacity of lactic acid bacteria isolated from Vietnamese feces and the Korean traditional food kimchi. Six isolated strains were identified as Lactobacillus sp. by 16S rRNA sequencing. All strains showed good resistance to low pH (1.5, 2.0, and 3.0) and 0.3% oxgall bile acids. Culture filtrates from the six strains showed various antioxidant effects, including DPPH, ABTS, reducing power, and metal chelating ($Fe^{2+}$) activities. Two of the six Lactobacillus strains showed potential probiotic activity. Heat resistance and adhesion assays were conducted by mixing the selected strains, Lactobacillus acidophilus V4, Lactobacillus plantarum V7, and Lactobacillus paracasei DK121 isolated from kimchi. The results showed that the heat resistance of these strains was similar to that of a commercial strain, L. plantarum LP. In addition, a mucin attachment assay using the mixture of selected strains (V4, V7, and DK121) showed high binding activity to the mucous layer. In conclusion, a mixture of V4, V7, and DK121 shows promising probiotic activity and may be useful for the development of health-related products.

본 연구에서는 베트남인의 분변으로부터 분리한 유산균의 probiotics 특성을 연구하기 위해 형태학적, 생화학적 특성조사 및 내산성, 내담즙성이 뛰어난 6개의 균주를 선별하고 동정하였다. 6개의 균주의 16S rRNA 분석 결과, L. acidophilus V4, L. plantarum V7, V9, V10, V11, V12의 2개 group으로 동정되었다. 베트남인의 분변으로부터 분리한 유산균은 pH 1.5, 2.0, 3.0의 산성 및 담즙산(0.3% oxgall) 조건에서 내산성과 내담즙성을 측정한 결과, V4, V7, V9, V11, V12의 5개 균주에서 70% 이상의 우수한 내산성과 내담즙성을 확인하였다. 항산화 활성에서는 V4, V7이 상용균주인 L. paracasei BGP2와 비교하였을 때, 높은 항산화 활성을 보여 최종 2종을 한국전통식품으로부터 분리한 L. paracasei DK121와의 혼합균주 가능성을 알아보았다. 열 안정성 실험에서 V4, V7, DK121을 5:4:1(w/v)로 혼합한 혼합균주는 $55^{\circ}C$에서 5분 98.6%, 15분 76.4%, $65^{\circ}C$에서 5분 66%, 15분 63.8%로 단일균주보다는 낮은 생존율을 보였지만, 향후 적합한 혼합도간 연구를 통해 프로바이오틱 균주로서 충분한 가능성을 보여주었다. 장내 부착능에서는 선발균주 및 혼합균주 모두 상용균주와 비교했을 때 6.4 log CFU/g 이상으로 우수한 결합능을 보여 향후 내열성과 안정성을 갖는 우수한 균주라고 판단된다.

Keywords

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