The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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Antinociceptive effect of intrathecal sec-O-glucosylhamaudol on the formalin-induced pain in rats

  • Kim, Sang Hun (Department of Anesthesiology and Pain Medicine, School of Medicine, Chosun University) ;
  • Jong, Hwa Song (Department of Anesthesiology and Pain Medicine, Chosun University Hospital) ;
  • Yoon, Myung Ha (Department of Anesthesiology and Pain Medicine, Medical School, Chonnam National University) ;
  • Oh, Seon Hee (Department of Premedics, School of Medicine, Chosun University) ;
  • Jung, Ki Tae (Department of Anesthesiology and Pain Medicine, School of Medicine, Chosun University)
  • Received : 2017.02.08
  • Accepted : 2017.02.27
  • Published : 2017.04.01
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Abstract

Background: The root of Peucedanum japonicum Thunb., a perennial herb found in Japan, the Philippines, China, and Korea, is used as an analgesic. In a previous study, sec-O-glucosylhamaudol (SOG) showed an analgesic effect. This study was performed to examine the antinociceptive effect of intrathecal SOG in the formalin test. Methods: Male Sprague-Dawley rats were implanted with an intrathecal catheter. Rats were randomly treated with a vehicle and SOG ($10{\mu}g$, $30{\mu}g$, $60{\mu}g$, and $100{\mu}g$) before formalin injection. Five percent formalin was injected into the hind-paw, and a biphasic reaction followed, consisting of flinching and licking behaviors (phase 1, 0-10 min; phase 2, 10-60 min). Naloxone was injected 10 min before administration of SOG $100{\mu}g$ to evaluate the involvement of SOG with an opioid receptor. Dose-responsiveness and ED50 values were calculated. Results: Intrathecal SOG showed a significant reduction of the flinching responses at both phases in a dose-dependent manner. Significant effects were showed from the dose of $30{\mu}g$ and maximum effects were achieved at a dose of $100{\mu}g$ in both phases. The ED50 value (95% confidence intervals) of intrathecal SOG was 30.3 $(25.8-35.5){\mu}g$ during phase 1, and 48.0 (41.4-55.7) during phase 2. The antinociceptive effects of SOG ($100{\mu}g$) were significantly reverted at both phases of the formalin test by naloxone. Conclusions: These results demonstrate that intrathecal SOG has a very strong antinociceptive effect in the formalin test and it seems the effect is related to an opioid receptor.

Keywords

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