Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhancement of Skin Antioxidant and Anti-Inflammatory Potentials of Agastache rugosa Leaf Extract by Probiotic Bacterial Fermentation in Human Epidermal Keratinocytes

프로바이오틱 유산균 발효에 의한 배초향 잎 추출물의 피부 항산화 및 항염증 활성 증대

  • Received : 2017.02.01
  • Accepted : 2017.03.24
  • Published : 2017.03.28
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Abstract

This study aimed to investigate the effects of probiotic fermentation by comparing the skin antioxidant and anti-inflammatory properties of non-fermented (ARE) and fermented (ARE-F) hot water extracts of Agastache rugosa leaves. ARE-F was obtained via ARE fermentation using Lactobacillus rhamnosus HK-9. In vitro, anti-inflammatory properties were evaluated by analyzing the levels of nitric oxide (NO), reactive oxygen species (ROS), and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated HaCaT keratinocytes. In vitro antiradical activity was measured using 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Attenuation of LPS-stimulated NO (p < 0.01), ROS (p < 0.001) and iNOS (p < 0.05) levels by ARE-F was significantly stronger than that by ARE in HaCaT keratinocytes. However, no differences were observed between the DPPH radical scavenging activities of ARE and ARE-F. ARE-F possesses enhanced skin antioxidant and anti-inflammatory properties, suggesting that probiotic bacterial fermentation can be considered an effective tool for augmenting some pharmacological properties of A. rugosa leaves. In brief, the skin antioxidant and anti-inflammatory potentials of A. rugosa leaf extract are augmented by the fermentation with L. rhamnosus HK-9, a probiotic bacterium.

본 연구에서, 비발효(ARE) 및 발효(ARE-F) 배초향 잎 열수 추출물의 피부 항산화 및 항염증 효능을 비교함으로써 프로바이오틱 발효의 효과를 검토하였다. ARE-F는 Lactobacillus rhamnosus HK-9 발효에 의하여 ARE로부터 제조되었다. In vitro 항염증 효능은 지질다당류(LPS)에 의하여 자극된 HaCaT 각질세포에서 일산화질소(NO), 활성산소종(ROS) 및 유도형 일산화질소합성효소(iNOS) 분석에 의하여 평가되었다. In vitro antiradical 활성은 2,2-diphenyl-2-picrylhydrazyl radical (DPPH) 소거 측정법에 의하여 평가되었다. LPS 자극에 의하여 증가된 NO, ROS 및 iNOS 수준에 미치는 ARE-F의 감소효과가 ARE에 의한 효과보다 현저히 강하였다. 그러나, ARE와 ARE-F의 DPPH 소거 효능에는 차이가 발견되지 않았다. ARE-F가 증강된 피부 항산화 및 항염증 효능을 갖고 있는데, 이 결과는 프로바이틱세균 발효가 배초향 잎의 일부 약리학적 효능을 증가시키는 효과적인 방법일 수 있음을 암시해 주고 있다. 요약하면, 배초향 잎 추출물의 피부항산화 및 항염증 활성이 프로바이오틱 세균인 L. rhamnosus HK-9에 의한 발효에 의하여 증강한다.

Keywords

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