The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant Activity and Melanin Inhibitory Effects of Yambean (Pachyrhizus erosus) Extract

얌빈 추출물의 항산화 효능과 멜라닌 생성 억제효과

  • Lee, AhReum (College of Korean Medicine, Daegu Haany University) ;
  • Kim, Gyo-Nam (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Hae-Ok (Department of Nursing, Kyungnam University) ;
  • Song, WeonJung (Sangju-si Agricultural Technology Center) ;
  • Roh, Seong-Soo (College of Korean Medicine, Daegu Haany University)
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김교남 (경남대학교 식품생명학과) ;
  • 김혜옥 (경남대학교 간호학과) ;
  • 송원정 (상주시농업기술센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2017.02.14
  • Accepted : 2017.03.15
  • Published : 2017.03.30
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Abstract

Objectives : Yam bean (Pachyrhizus erosus) possess various nutrients, it has been widely used as traditional cosmetic material in Indonesia. The aim of this study was to investigate the anti-oxidant activity and the anti-melanogenic effect of Yambean (Pachyrhizus erosus) extract and its fractions. Methods : The anti-oxidant activity of yam bean extract assessed based on total polyphenol, flavonoid contents, DPPH and ABTS radical scavenging assay. To evaluate anti-melanogenic effects and cytotoxicity of Yambean extract and its fractions, B16F10 melanoma cell was used. Results : In results, total polyphenol content of yam bean water extract (YW) and Yambean 70% ethanol extract (YE) were $1.18{\pm}0.03mg/g$ (mg of gallic acid/g of sample), $1.16{\pm}0.01mg/g$. Total flavonoid contents of YW, YE were $3.55{\pm}0.06mg/g$ (mg of naringin/g of sample), $1.78{\pm}0.03mg/g$. Moreover, YE scavenged DPPH and ABTS effectively in $4mg/m{\ell}$ compared to YW. Cytotoxicity of YE and its fractions in B16F10 melanoma cell was measured using MTT assays. It had no cytotoxicity up to $500{\mu}g/m{\ell}$. Melanin accumulation in B16F10 melanoma cell was induced using alpha-melanocyte stimulating hormone (${\alpha}-MSH$) and 3-isobutyl-1-methylxanthine (IBMX). B16F10 melanoma cell treated with $10-500{\mu}g/m{\ell}$ YE and hexane, ethyl acetate, butanol, $H_2O$ fractions for 24h. Non treated B16F10 melanoma cell (Control) markedly increased melanin contents. In contrast, YE ethylacetate fraction effectively suppressed melanin accumulation in a dose-dependent manner. Conclusion : In conclusion, these results suggest that Yambean extract has the potential as a cosmetic material which possess anti-oxidant and anti-melanogenic activities.

Keywords

References

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