Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Identification of the Component with Anti-acetylcholinesterase Activity from the Essential Oil of Artemisia iwayomogi

더위지기 정유로부터 아세틸콜린에스테라제 억제활성 성분의 동정

  • Choi, Jae Sue (Department of Food Science and Nutrition, Pukyong National University) ;
  • Song, Byong-Min (Department of Forest Science, Sangji University) ;
  • Park, Hee-Juhn (epartment of Pharmaceutical Engineering, Sangji University)
  • Received : 2016.09.02
  • Accepted : 2016.11.09
  • Published : 2017.02.28
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Abstract

Since the acetylcholinesterase (AChE) inhibitor is used to treat Alzheimer's disease, the present study aimed to analyze the component with anti-AChE activity from the essential oil of Artemisia iwayomogi (Compositae). The four major components of the essential oil were identified to be camphor (29.8%), borneol (28.0%), eucalyptol (5.81%) and coumarin (5.49%) from a gas chromatography-mass spectrometry (GC-MS). The essential oil and its three components, camphor, borneol, and coumarin, were subjected to anti-AChE assay. The $IC_{50}$ values of the essential oil and coumarin were shown to be $0.298mg/m{\ell}$ and $0.236mg/m{\ell}$, though those of other two components, camphor and borneol, were more than $0.250mg/m{\ell}$. These results suggest that coumarin is an active substance of this essential oil with anti-AChE activity.

AChE 억제제는 알츠하이머 질환 치료에 이용될 수 있으므로, 본 연구는 더위지기 정유로부터 AChE 활성성분을 탐색하기 위하여 수행한 것이다. 더위지기로부터 얻은 정유를 GC-MS로 분석했을 때 camphor가 29.8%, borneol이 28.0%, eucalyptol이 5.81%, coumarin이 5.49%의 피크 면적 비율을 보였다. 이 중 camphor, borneol, coumarin을 사용하여 Anti-AChE 활성 검색을 수행하였다. 그 결과, $0.298mg/m{\ell}$, coumarin은 $0.236mg/m{\ell}$의 $IC_{50}$ 값을 나타내었다. 이러한 결과는 coumarin이 anti-AChE 활성을 나타내는 활성물질임을 나타내는 것이다.

Keywords

References

  1. Chaiyana, W. and S. Okonogi. 2012. Inhibition of cholinesterase by essential oil from food plant. Phytomedicine 19:836-839. https://doi.org/10.1016/j.phymed.2012.03.010
  2. Ehrnhoofer-Ressler, M.M., K. Fricke, M. Pignitter, J.M. Walker, J. Walker, M. Rychlik and V. Somoza. 2013. Identification of 1,8-cineole, borneol, camphor, and thujone as anti-inflammatory compounds in a Salvia officinalis L. infusion using human gingival fibroblasts. J. Agric. Food Chem. 61:3451-3459. https://doi.org/10.1021/jf305472t
  3. Ellman, G. L., K.D. Courtney, V.J. Andres and R.M. Feather-stone. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  4. Lai, T.S., W.F. Wang, B.S. Yip, Y.W. Yang, G.S. Peng, S.J. Tsai, Y.C. Liao and M.C. Pai. 2016. Real-world evaluation of compliance and preference in Alzheimer's disease treatment: an observational study in Taiwan. Patient Pref. Adherence 10:383-390.
  5. Lopez, M.D., F.J. Campoy, M.J. Pascual-Villalobos, E. Munoz-Delgado and C.J. Vidal. 2015. Acetylcholinesterase activity of electric eel is increased or decreased by selected monoterpenoids and phenylpropanoids in a concentration dependent manner. Chem. Biol. Interac. 229:36-43. https://doi.org/10.1016/j.cbi.2015.01.006
  6. Nugroho, A., S.C. Lim and H.J. Park. 2012. Qualitative analysis of phenolic substances in Artemisia capillaris by LC-MS. Kor. J. Pharmacogn. 43:302-307.
  7. Nugroho, A., S.C. Lim, S. Karki, J.S. Choi and H.J. Park. 2015. Simultaneous quantification and validation of new peroxynitrite scavengers from Artemisia iwayomogi. Pharmaceut. Biol. 52:653 - 661.
  8. Salleh, W.M., F. Ahmad and K.H. Yen. 2015. Antioxidant and anticholinesterase activities of essential oils of Cinnamomum griffithii and C. macrocarpum. Nat. Prod. Commun. 10:1465-1468.
  9. Seo, K.S. and K.W. Yun. 2008. Antioxidant activities of extracts from Artemisia capillaris THUNB. and Artemisia iwayomogi KITAM. used as Injin. Korean J. Plant Res. 21:292-298.
  10. Syad, A.N. and K.P. Devi. 2014. Botanics: a potential source of new therapies for Alzheimer's disease? Bot. Targ. Ther. 4:11-26.
  11. Passos, G.F., E.S. Fernandes, F.M. da Cunha, J. Ferreira, L.F. Pianowski, M.M. Campos and J.B. Calixto. 2007. Anti-inflammatory and anti-allergic properties of the essential oil and active compounds from Cordia verbenacea. J. Ethnopharmacol. 110:323-33. https://doi.org/10.1016/j.jep.2006.09.032
  12. Tsui, W.Y. and G.D. Brown. 1996. Sesquiterpenes from Baeckea frutescens, J. Nat. Prod. 59:1084-1086. https://doi.org/10.1021/np960419w
  13. Wang, J.H., L. Xu, L. Yang, Z.L. Liu and L. Zhou. 2011. Composition, antibacterial and antioxidant activities of essential oils from Ligusticum sinense and L. jeholense (Umbelliferae) from China. Rec. Nat. Prod. 5:314-318.
  14. Yu, H.H., Y.H. Kim, B.S. Kil, K.J. Kim, S.I. Jeong and Y.O. You. 2003. Chemical composition and antibacterial activity of essential oil of Artemisia iwayomogi. Planta Med. 69:1159-1162. https://doi.org/10.1055/s-2003-818011

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