Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Yeasts in Internal Roots of the Rare Plant Dendropanax morbifera

  • Kim, Jong-Shik (Gyeongbuk Institute for Marine Bio-Industry (GIMB)) ;
  • Kim, Dae-Shin (World Heritage Office, Jeju Special Self-Governing Provincial Government) ;
  • Ko, Suk-Hyung (World Heritage Office, Jeju Special Self-Governing Provincial Government)
  • Received : 2017.01.25
  • Accepted : 2017.03.20
  • Published : 2017.03.30
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Abstract

To isolate and identify the yeast strains associated with D. morbifera, homogenized D. morbifera root samples were spread onto GPY, DG18, SCG and DOB agar media containing antibiotics, Triton X-100, and l-sorbose. Total 81 yeast isolates were analyzed by sequencing of internal transcribed spacer (ITS) region of the ribosomal DNA. The results showed that the root-associated yeast species were composed of the genera Vanderwaltozyma (40 isolates), Cryptococcus (40 isolates), and Kluyveromyces (one isolate). Moreover, the Kluyveromyces isolate exhibited high bioethanol productivity. In addition, the Vanderwaltozyma and Cryptococcus were dominant in D. morbifera roots. The specific yeast community associated with D. morbifera roots was identified by phylogenetic sequence analyses. These yeast isolates may have industrial applications as biosurfactant and bioethanol.

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References

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