Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Canna edulis Leaf Extract-Mediated Preparation of Stabilized Silver Nanoparticles: Characterization, Antimicrobial Activity, and Toxicity Studies

  • Otari, S.V. (Department of Chemical Engineering, Konkuk University) ;
  • Pawar, S.H. (Center for Interdisciplinary Research, D. Y. Patil University) ;
  • Patel, Sanjay K.S. (Department of Chemical Engineering, Konkuk University) ;
  • Singh, Raushan K. (Department of Chemical Engineering, Konkuk University) ;
  • Kim, Sang-Yong (Department of Food Science and Biotechnology, Shin-Ansan University) ;
  • Lee, Jai Hyo (Department of Mechanical Engineering, Konkuk University) ;
  • Zhang, Liaoyuan (Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Life Sciences, Gutian Edible Fungi Research Institute, Fujian Agriculture and Forestry University) ;
  • Lee, Jung-Kul (Department of Chemical Engineering, Konkuk University)
  • Received : 2016.10.10
  • Accepted : 2017.01.11
  • Published : 2017.04.28
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Abstract

A novel approach to synthesize silver nanoparticles (AgNPs) using leaf extract of Canna edulis Ker-Gawl. (CELE) under ambient conditions is reported here. The as-prepared AgNPs were analyzed by UV-visible spectroscopy, transmission emission microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, energy-dispersive analysis of X-ray spectroscopy, zeta potential, and dynamic light scattering. The AgNPs showed excellent antimicrobial activity against various pathogens, including bacteria and various fungi. The biocompatibility of the AgNPs was analyzed in the L929 cell line using NRU and MTT assays. Acridine orange/ethidium bromide staining was used to determine whether the AgNPs had necrotic or apoptotic effects on L929 cells. The concentration of AgNPs required for 50% inhibition of growth of mammalian cells is far more than that required for inhibition of pathogenic microorganisms. Thus, CELE is a candidate for the eco-friendly, clean, cost-effective, and nontoxic synthesis of AgNPs.

Keywords

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