Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Fermentation of Environmental Friend Total Mixed Ration and Alteration of Rumen Fermentation Characteristics

환경친화적 섬유질 배합사료의 발효와 반추위 발효특성 변화

  • 류채화 (전북대학교 축산학과) ;
  • 박명선 (전북대학교 축산학과) ;
  • 박철 (전북대학교 수의학과) ;
  • 최낙진 (전북대학교 동물자원과학과) ;
  • 조상범 ((주)칼스엔비티 순환농축산연구소, 전북대학교 동물자원과학과)
  • Received : 2017.03.29
  • Accepted : 2017.05.10
  • Published : 2017.05.31
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Abstract

Total mixed ration (TMR) including concentrate diet and roughage together have been used for the ruminant animal. Relatively high concentrations of moisture and water soluble carbohydrate are representative feature of TMR. Those moisture and water can also provide a niche for bacterial growth. Therefore, a possible fermentation of TMR induced by micro-organism is generally accepted. The present study hypothesized that different lactic acid bacteria could alter fermentation of TMR and subsequently rumen fermentation. Three lactic acid bacteria, Lactobacillus paracasei (A), L. plantarum (B) and L. parabuchneri (C), were employed and 7 treatments under full factorial design were compared with control without inoculation. TMR for dairy cow was used. Significant alterations by treatments were detected at lactic acid and butyric acid contents in TMR (p<0.05). Treatment AC (mixture of A and C) and BC (mixture of B and C) showed great lactate production. Great butyrate production was found at treatment C. At in vitro rumen fermentation, treatments B, C and AB (mixture of A and B) showed significantly great total gas production (p<0.05). All treatments except treatments B and AB, showed less dry matter digestibility, significantly (p<0.05). Total volatile fatty acid production at treatment AC was significantly greater than others (p<0.05). In individual volatile fatty acid production, treatment AB and AC showed great acetate and propionate productions, significantly (p<0.05). This study investigated correlation between organic acid production in TMR and rumen volatile fatty acid production. And it was found that butyric acid in TMR had significant negative correlation with acetate, propionate, total volatile fatty acid, AP ratio and dry matter digestibility.

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References

  1. AOAC. 1995. Official methods of analysis. Association of Official Analytical Chemists, Washington DC.
  2. Bannink, A., J. Kogut, J. Dijkstra, J. France, E. Kebreab, A. M. Van Vuuren, and S. Tamminga. 2006. Estimation of the stoichiometry of volatile fatty acid production in the rumen of lactating cows. J. Theor. Biol. 238(1): 36-51. https://doi.org/10.1016/j.jtbi.2005.05.026
  3. Cho, S.-B., S.-M. Lee, and E.-J. Kim. 2012. Effect of Different Forages on Growth Performance, Meat Production and Meat Quality of Hanwoo Steers: Meta-analysis. J. Kor. Grassl. Forage Sci. 32(2): 175-184. https://doi.org/10.5333/KGFS.2012.32.2.175
  4. Cho, S., J. S. Kang, K. J. Cho, K. H. Lee, C. H. Kwon, J. Song, K. Lee, S. Y. Kim, and E. J. Kim. 2014. Effect of homofermentative and heterofermentative lactic acid bacteria on the quality and aerobic stability of silage: Meta-analysis. J. Kor. Grassl. Forage Sci. 34(4): 247-253. https://doi.org/10.5333/KGFS.2014.34.4.247
  5. Dijkstra, J., T. M. Forbes, and J. France. 2005. Quantitative aspects of ruminant digestion and metabolism. Second edition. CABI Publishing, Cambridge, MA 02139. pp. 159-160.
  6. Lee, A. L., S.-J. Shin, J. Yang, S. Cho, and N.-J. Choi. 2016. Effect of Lactic acid bacteria and Enzyme Supplementation on Fermentative Patterns of Ensiling Silages, Their In vitro Ruminal Fermentation, and Digestibility. J. Kor. Grassl. Forage Sci. 36(1): 7-14. https://doi.org/10.5333/KGFS.2016.36.1.7
  7. Lee, S. J., N. H. Shin, J. H. Hyun, T. W. Kang, J. J. An, H. S. Jung, Y. H. Moon, and S. S. Lee. 2009. Effects of supplementation of synbiotic co-cultures manufactured with anaerobic microbes on in vitro fermentation characteristics and in situ degradability of fermented TMR. J. Life Sci. 19(11): 1538-1546. https://doi.org/10.5352/JLS.2009.19.11.1538
  8. Liu, Q., X. Li, S. T. Desta, J. Zhang, and T. Shao. 2016. Effects of Lactobacillus plantarum and fibrolytic enzyme on the fermentation quality and in vitro digestibility of total mixed rations silage including rape straw. J. Integrative Agric. 15(9): 2087-2096. https://doi.org/10.1016/S2095-3119(15)61233-3
  9. McDougall, E. 1948. Studies on ruminant saliva. 1. The composition and output of sheep's saliva. Biochem. J. 43:99-109. https://doi.org/10.1042/bj0430099
  10. Morvay, Y., A. Bannink, J. France, E. Kebreab, and J. Dijkstra. 2011. Evaluation of models to predict the stoichiometry of volatile fatty acid profiles in rumen fluid of lactating Holstein cows. J. Dairy Sci. 94(6): 3063-3080. https://doi.org/10.3168/jds.2010-3995
  11. Reynal, S. M. and G. A. Broderick. 2005. Effect of dietary level of rumen-degraded protein on production and nitrogen metabolism in lactating dairy cow. J. Dairy Sci. 88: 4045-4046. https://doi.org/10.3168/jds.S0022-0302(05)73090-3
  12. Seymour, W. M., D. R. Campbell, and Z. B. Johnson. 2005. Relationships between rumen volatile fatty acid concentrations and milk production in dairy cows: a literature study. Anim. Feed Sci. Tech. 119(1-2): 155-169. https://doi.org/10.1016/j.anifeedsci.2004.10.001
  13. Team, R. D. C. 2010. R: A language and environment for statistical computing. R Foundation for Statistical Computing. Retrived from http://www.R-project.org, Vienna, Austria.
  14. Van Soest, P. V., J. Robertson, and B. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysacchrides in relation to animal nutrition. J. Dairy Sci. 74: 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2