Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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S100A8 and S100A9 Secreted by Allergens in Monocytes Inhibit Spontaneous Apoptosis of Normal and Asthmatic Neutrophils via the Lyn/Akt/ERK Pathway

단구에서 분비되는 S100A8과 S100A9의 Lyn/Akt/ERK 경로를 통한 정상인과 천식질환 호중구의 세포고사 억제 효과

  • Kim, In Sik (Department of Biomedical Laboratory Science, School of Medicine, Eulji University) ;
  • Lee, Ji-Sook (Department of Clinical Laboratory Science, Wonkwang Health Science University)
  • 김인식 (을지대학교 대전캠퍼스 임상병리학과) ;
  • 이지숙 (원광보건대학교 임상병리과)
  • Received : 2017.04.13
  • Accepted : 2017.05.29
  • Published : 2017.06.30
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Abstract

Der p 1 and Der p 2 are essential allergens of house dust mite associated with the development of asthma. In the present study, we examined whether Der p 1 and Der p 2 induce a release of S100A8 and S100A9 in monocytes, which are involved in the regulatory mechanism of neutrophil apoptosis. We found that Der p 1 and Der p 2 significantly increased the secretion of S100A8 and S100A9 in normal monocytes. Moreover, S100A8 and S100A9 strongly suppressed the spontaneous apoptosis of normal and asthmatic neutrophils. The inhibitory effect of S100A9 was stronger than that of S100A8, and asthmatic neutrophils showed a higher inhibitory effect than normal neutrophils. S100A8 and S100A9 induced activation of Lyn, Akt, and ERK in a time-dependent manner. These findings elucidate the roles of Der p 1 and Der p 2 in the interaction between monocytes and neutrophils, as well as contributing to our knowledge of the pathogenesis of allergic diseases.

Der p 1과 Der p 2는 알레르기 질환과 관련된 집먼지 진드기의 핵심적인 알러젠이다. 본 연구에서는 Der p 1과 Der p 2가 단구에서 S100A8과 S10A9을 분비시키는지를 확인하였고, 분비된 S100A8과 S10A9이 호중구의 세포고사 조절기전에 작용하는지를 연구하였다. Der p 1과 Der p 2는 정상인의 단구에서 S100A8과 S10A9을 유의하게 증가시켰고, S100A8과 S10A9은 정상인과 알레르기 질환 호중구의 자발적 세포고사를 억제 시켰다. 호중구의 Lyn, Akt, ERK는 S100A8과 S10A9을 시간별로 처리하였을 때 활성화하였다. 본 연구를 통하여 단구와 호중구에서 Der p 1과 Der p 2의 역할을 규명하였고, 나아가 관련된 알레르기 병인기전을 이해하는데 유용할 것이다.

Keywords

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