Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Gonadal Maturation, RNA/DNA Ratio of Mature Eggs, and the Effect of Refrigeration on Egg Viability and Sperm Motility in Korean Walleye pollock Theragra chalcogramma

동해안 북부 자망에서 어획된 명태(Theragra chalcogramma)의 생식소 성숙과 포획 후 경과시간에 따른 성숙란의 RNA/DNA ratio 및 냉장보관 정자의 활력

  • 서주영 (강원도한해성수수산자원센터) ;
  • 권오남 (강원양식생물연구소(주))
  • Received : 2016.09.20
  • Accepted : 2017.06.14
  • Published : 2017.06.30
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Abstract

We conducted a study to 1) determine the indicators of gonadal maturity in male and female Korean walleye pollock Theragra chalcogramma for the purposes of artificial insemination; 2) establish the RNA/DNA ratio of mature eggs in this species; and 3) monitor the effect of refrigerated storage on egg viability and the motility of sperm collected from dead adult males. During the spawning season, the color of female gonads changed from orange to transparent, and that of male gonads changed from pale orange to milky white. The DNA content and RNA/DNA ratio of mature eggs were maintained without significant changes for approximately 6 h when eggs were preserved at $4^{\circ}C$. Sperm could be obtained from both milt and undiluted semen. Sperm obtained from milt ceased moving on the second day after isolation, while over 60% of sperm obtained from semen showed movement until the 13th day. Seven attempts were made to artificially inseminate mature eggs, of which two resulted in successful fertilization. The successful inseminations produced 94,000 and 5,000 fertilized eggs, respectively. This study shows that artificial insemination of walleye pollock is a viable strategy when natural propagation is not possible.

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References

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