Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Suppression of brown blotch disease by tolaasin inhibitory factors

톨라신 저해 물질을 이용한 갈반병의 억제

  • Yun, Yeong-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kim, Min-Hee (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Han, Ji-Hye (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • Received : 2017.06.09
  • Accepted : 2017.06.15
  • Published : 2017.06.30
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Abstract

Tolaasin, a 1.9 kDa peptide toxin, is produced by Pseudomonas tolaasii and causes the brown blotch disease of cultivated oyster mushroom. It forms pores on the membrane and thus destroys cellular membrane structure, seriously reducing the productivity of mushroom cultivation. The mechanism of tolaasin-induced cytotoxicity is not known in detail. However, it has been reported to form a pore structure in the cytoplasmic membrane through the molecular multimerization. Therefore, food additives which can interact with tolaasin molecules may inhibit the pore formation by hydrophobic interactions with tolaasin molecules. In this study, various food additive materials have been identified as inhibitors of the tolaasin activity and named tolaasin-inhibitory factors (TIF). Most of TIFs are emulsifying agents for food processing procedures. Among various TIFs, polyglycerol and sucrose esters of fatty acids blocked effectively the cytotoxicity of tolaasins at the concentrations $10^{-4}-10^{-5}M$. These TIFs also successfully suppressed the blotch disease development in the shelf cultivation of oyster mushroom.

톨라신은 1.9 kDa의 펩티드 독소로서 Pseudomonas tolaasii에 의해 생성되며, 재배중 느타리버섯에 갈반병을 일으킨다. 톨라신은 막에 pore를 형성하여 세포 구조를 파괴하고, 버섯 재배의 생산성을 심하게 감소시킨다. 톨라신에 의한 세포독성의 작용 기작은 완전히 밝혀지지 않았지만, 분자다중화에 의해 세포막에 채널구조 형성으로 이루어진다. 그러므로, 톨라신과 작용하는 식품첨가물 중에 톨라신의 다중화결합을 통한 세포막 pore 형성을 저해하는 물질이 있을 것이다. 본 연구에서는, 다양한 물질들이 톨라신의 활성을 저해함을 확인하고, 이들을 톨라신 저해물질(TIF)이라 명명하였다. 대부분의 톨라신 저해물질들은 식품가공과정에 쓰이는 유화제였다. 다양한 종류의 저해물질 중에 지방산과 에스터 결합한 polyglycerol과 지방산과 에스터 결합한 sucrose 화합물이 $10^{-4}-10^{-5}M$ 농도범위에서 톨라신의 세포독성을 효과적으로 저해하였다. 이러한 저해물질들은 균상재배하는 느타리버섯에서 갈반병의 발생을 성공적으로 억제하였다.

Keywords

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