Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Draft genome sequence of Lactobacillus salivarius KLW001 isolated from a weaning piglet

이유자돈으로부터 분리한 Lactobacillus salivarius KLW001의 유전체 분석

  • Jin, Gwi-Deuk (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Jun-Yeong (Department of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Eun Bae (Department of Animal Life Science, Kangwon National University)
  • 진귀득 (강원대학교 동물생명과학과) ;
  • 이준영 (서울대학교 농생명공학부) ;
  • 김은배 (강원대학교 동물생명과학과)
  • Received : 2017.05.10
  • Accepted : 2017.05.17
  • Published : 2017.06.30
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Abstract

Lactobacillus salivarius KLW001, a species of lactic acid bacteria (LAB), was isolated from a weaning piglet in a swine farm, South Korea, to develop an antimicrobial probiotic strain for piglets. Herein, we report the draft genome sequence of the strain. The genome contains 2,326,706 bp with a G+C content of 33.0% in 166 contigs (${\geq}500bp$). From the genome, we found out 4 genes related to antibiotic resistance, 36 genes for phages, 3 genes for bile hydrolysis, and 27 CRISPR spacers.

이유자돈용 생균제 개발을 위해, 본 연구자들은 유산균의 일종인 Lactobacillus salivairus KLW001 균주를 대한민국 양돈가에서 사육 중인 이유자돈으로부터 분리하였다. 이 균주는 K88 antigen-positive Escherichia coli, Salmonella enterica serovar Typhimurium에 대한 항균 활성이 타 균주보다 우수하여, 우리는 이 균주의 유전체를 분석하였다. 유전체 초안 속의 166개 Contig (${\geq}500bp$)들에서, G+C content (%)가 33.0%였고, 2,326,706 bp 크기의 염기서열을 확보할 수 있었다. 유전체 초안으로부터 항생제 저항 유전자 4개, Phage 관련 유전자 36개, Bile 대사 유전자 3개, CRISPR Spacer 27개를 확인하였다.

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References

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