Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-inflammatory Effects of Aster yomena Extracts by the Suppression of Inducible Nitric Oxide Synthase Expression

  • Kim, Ah-Yeon (Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University) ;
  • Shin, Hyeon-Myeong (Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University) ;
  • Kim, Ji-Soo (Departments of Medical Science, College of Medical Sciences, SoonChunHyang University) ;
  • Shim, Hyun-Jin (Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University) ;
  • Nam, Kung-Woo (Department of Life Science and Biotechnology, College of Natural Science, Soonchunhyang University) ;
  • Hwang, Kyung-A (Department of Agrofood Resources, National Academy of Agricultural Science, RDA) ;
  • Youn, Hyung-Sun (Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University)
  • Received : 2017.05.12
  • Accepted : 2017.06.28
  • Published : 2017.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Inflammation is a pathophysiological process that is known to be involved in numerous diseases. Microbial infection or tissue injury activates inflammatory responses, resulting in the induction of proinflammatory proteins including inducible nitric oxide synthase (iNOS). Aster yomena is used in traditional Korean remedies. Here, we investigated the effects of ethanol extracts of Aster yomena (EAY) on the expression of iNOS induced by ovalbumin (OVA), one of the major egg allergens, or lipopolysaccharide (LPS), a Toll-like receptor 4 agonist. EAY inhibited OVA- or LPS-induced $NF-{\kappa}B$ activation. EAY also suppressed OVA- or LPS-induced iNOS expression and nitrite production. These results suggest that EAY has the specific mechanism for anti-inflammatory responses and the potential to be developed as a potent anti-inflammatory and anti-allergic drug.

Keywords

References

  1. Ahn SI, Lim SJ, Gu GJ, Hong CY, Kim JS, Jeong HJ, Koh KO, Mang JY, Kim DY, Youn HS. Suppressive effects of 1-[4-fluoro-2-(2-nitrovinyl)phenyl]pyrrolidine on the Toll-like receptor signaling pathways. International Immunopharmacology. 2015. 24: 36-41. https://doi.org/10.1016/j.intimp.2014.10.033
  2. Akira S, Takeda K. Toll-like receptor signaling. Nature Review Immunology. 2004. 4: 499-511. https://doi.org/10.1038/nri1391
  3. Bjorkbacka H, Fitzgerald KA, Huet F, Li X, Gregory JA, Lee MA, Ordija CM, Dowley NE, Golenbock DT, Freeman MW. The induction of macrophage gene expression by LPS predominantly utilizes Myd88-independent signaling cascades. Physiology Genomics. 2004. 19: 319-330. https://doi.org/10.1152/physiolgenomics.00128.2004
  4. Burney S, Caulfield JL, Niles JC, Wishnok JS, Tannenbaum SR. The chemistry of DNA damage from nitric oxide and peroxynitrite. Mutation Research. 1999. 424: 37-49. https://doi.org/10.1016/S0027-5107(99)00006-8
  5. Eggesbo M, Botten G, Halvorsen R, Magnus P. The prevalence of allergy to egg: a population-based study in young children. Allergy. 2001. 56: 403-411. https://doi.org/10.1034/j.1398-9995.2001.056005403.x
  6. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clinical & Experimental Immunology. 2007. 147: 227-235.
  7. Gao JJ, Filla MB, Fultz MJ, Vogel SN, Russell SW, Murphy WJ. Autocrine/paracrine IFN-alphabeta mediates the lipopolysaccharide-induced activation of transcription factor Stat1alpha in mouse macrophages: pivotal role of Stat1alpha in induction of the inducible nitric oxide synthase gene. Journal of Immunology. 1998. 161: 4803-4810.
  8. Green SJ, Meltzer MS, Hibbs JB Jr, Nacy CA. Activated macrophages destroy intracellular Leishmania major amastigotes by an L-arginine-dependent killing mechanism. Journal of Immunology. 1990. 144: 278-283.
  9. Hanafy KA, Krumenacker JS, Murad F. NO, nitrotyrosine, and cyclic GMP in signal transduction. Medical Science Monitor. 2001. 7: 801-819.
  10. Kawai T, Akira S. Signaling to NF-kappaB by Toll-like receptors. Trends in Molecular Medicine. 2007. 13: 460-469. https://doi.org/10.1016/j.molmed.2007.09.002
  11. Kim AR, Jin Q, Jin HG, Ko HJ, Woo ER. Phenolic compounds with IL-6 inhibitory activity from Aster yomena. Archives Pharmacal Research. 2014. 37: 845-851. https://doi.org/10.1007/s12272-013-0236-x
  12. Lee AN, Park SJ, Jeong AR, Lee JR, Park HJ, Kim SJ, Min IS, Youn HS. Ovalbumin induces cycloxygenase-2 and inducible nitric oxide synthase expression. Korean Journal of Food Science Technology. 2011. 43: 110-113. https://doi.org/10.9721/KJFST.2011.43.1.110
  13. Lim HJ, Lee HS, Ryu JH. Suppression of inducible nitric oxide synthase and cyclooxygenase-2 expression by tussilagone from Farfarae flos in BV-2 microglial cells. Archives Pharmacal Research. 2008. 31: 645-652. https://doi.org/10.1007/s12272-001-1207-4
  14. Mine Y, Yang M. Recent advances in the understanding of egg allergens: basic, industrial, and clinical perspectives. Journal of Agricultural and Food Chemistry. 2008. 56: 4874-4900. https://doi.org/10.1021/jf8001153
  15. Moncada S. Nitric oxide: discovery and impact on clinical medicine. Journal of the Royal Society Medicine. 1999. 92: 164-169. https://doi.org/10.1177/014107689909200402
  16. Pahl HL. Activators and target genes of Rel/NF-kappaB transcription factors. Oncogene. 1999. 18: 6853-6866. https://doi.org/10.1038/sj.onc.1203239
  17. Palmer RM, Hickery MS, Charles IG, Moncada S, Bayliss MT. Induction of nitric oxide synthase in human chondrocytes. Biochemical and Biophysical Research Communications. 1993. 193: 398-405. https://doi.org/10.1006/bbrc.1993.1637
  18. Stamler JS, Lamas S, Fang FC. Nitrosylation. the prototypic redoxbased signaling mechanism. Cell. 2001. 106: 675-683. https://doi.org/10.1016/S0092-8674(01)00495-0
  19. Takeda K, Akira S. Toll-like receptors in innate immunity. International Immunology. 2005. 17: 1-14.
  20. Turini ME, DuBois RN. Cyclooxygenase-2: a therapeutic target. Annual Review of Medicine. 2002. 53: 35-57. https://doi.org/10.1146/annurev.med.53.082901.103952
  21. Vallance P. Nitric oxide: therapeutic opportunities. Fundamental & Clinical Pharmacology. 2003. 17: 1-10. https://doi.org/10.1046/j.1472-8206.2003.00124.x
  22. Wang J, Sampson HA. Food allergy: recent advances in pathophysiology and treatment. Allergy Asthma & Immunology Research. 2009. 1: 19-29. https://doi.org/10.4168/aair.2009.1.1.19
  23. Youn HS. The anti-inflammatory effects of phytochemicals by the modulation of innate immunity. Journal of Experimental & Biomedical Sciences. 2012. 18: 181-192.
  24. Youn HS, Lee JY, Saitoh SI, Miyake K, Hwang DH. Auranofin, as an anti-rheumatic gold compound, suppresses LPS-induced homodimerization of TLR4. Biochemical and Biophysical Research Communications. 2006. 350: 866-871. https://doi.org/10.1016/j.bbrc.2006.09.097
  25. Youn HS, Lee JY, Saitoh SI, Miyake K, Kang KW, Choi YJ, Hwang DH. Suppression of MyD88- and TRIF-dependent signaling pathways of Toll-like receptor by (-)-epigallocatechin-3-gallate, a polyphenol component of green tea. Biochemical Pharmacology. 2006. 72: 850-859. https://doi.org/10.1016/j.bcp.2006.06.021
  26. Youn HS, Saitoh SI, Miyake K, Hwang DH. Inhibition of homodimerization of Toll-like receptor 4 by curcumin. Biochemical Pharmacolgy. 2006. 72: 62-69. https://doi.org/10.1016/j.bcp.2006.03.022