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Antioxidative activity of roasted Pueraria lobata root extracts

로스팅 칡 추출물의 항산화 활성

  • Choi, Goo-Hee (Hurum Central research institute Co., Ltd.) ;
  • Kim, Hyun Jung (Hurum Central research institute Co., Ltd.) ;
  • Park, In-Jae (Hurum Central research institute Co., Ltd.) ;
  • Kim, Bong-Gyun (Hurum Central research institute Co., Ltd.) ;
  • Kim, Hoi-Yeong (Food Science and Technology, Korea National University of Transportation) ;
  • Jeong, Jae-Hyun (Food Science and Technology, Korea National University of Transportation) ;
  • Cho, Ju-Hyun (Hurum Central research institute Co., Ltd.)
  • Received : 2017.04.07
  • Accepted : 2017.04.26
  • Published : 2017.06.30

Abstract

We evaluated the antioxidative activity of extracts of P. lobata root depending on roasting conditions. P. lobata roots were roasted at three different temperature at $150^{\circ}C$, $200^{\circ}C$, and $250^{\circ}C$ and three different time at 10 min, 20 min, and 30 min respectively. Roasted P. lobata root was extracted using water at $85^{\circ}C$ for 6 h and filtered using filter paper, followed by then evaporated ($12{\pm}0.3^{\circ}Brix$) and freeze-dried. The concentration of maker compound puerarin was determined using a high performance liquid chromatography system. 2 phenolic compounds, flavonoid contents, and antioxidant activities of the extract powder were evaluated. Puerarin contents, Phenolic compounds, and flavonoid contents of roasted P. lobata root were higher than those of unroasted P. lobata root. The results of DPPH and ABTS showed that roasted P. lobata root possessed higher antioxidant activity than unroasted P. lobata root. This study suggested that roasting process could be applied to P. lobata root in order to achieve its high quality and functionality.

본 연구에서는 기능성이 증대된 칡 소재 개발을 위하여 로스팅 조건에 따른 칡 추출물을 제조하였으며, 각 추출물에 대한 지표성분 함량 및 항상화 활성 등을 검토하였다. 칡의 지표성분이 puerarin 함량을 로스팅 조건에 따라 분석을 실시하였으며, 그 결과 로스팅 온도와 시간이 늘어남에 따라 puerarin 함량이 증가하는 경향을 나타내었다. 또한, 로스팅 조건에 따른 총 페놀과 플라보노이드 함량의 경우도 puerarin 함량의 결과와 마찬가지로 유사한 경향을 보이고 있었다. 항산화 활성을 평가하기 위하여 ABTS 및 DPPH 라디칼 소거능을 실시하였으며, 전체적으로 로스팅 온도와 시간이 늘어남에 따라 활성이 높아지는 경향을 보여 로스팅 처리한 칡이 일반적인 칡 보다 우수한 것으로 나타났다. 이러한 결과를 종합하여 볼 때 칡을 활용한 기능성 식품 및 다류 개발에 있어서, 로스팅 공정을 적용하는 것이 효과적인 것으로 생각된다.

Keywords

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