Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Comparative Analysis of Tissue and Cell Cycle on the Far Eastern Catfish, Silurus asotus between Diploid and Triploid

  • Gil, Hyun Woo (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Lee, Tae Ho (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University) ;
  • Han, Ho Jae (College of Veterinary Medicine and Research Institute of Veterinary Science, Seoul National University) ;
  • Park, In-Seok (Division of Marine Bioscience, College of Ocean Science and Technology, Korea Maritime and Ocean University)
  • Received : 2017.05.16
  • Accepted : 2017.05.24
  • Published : 2017.06.30
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Abstract

The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the $G_l-$, the S- and the $G_2+M-phase$ fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.

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References

  1. Aliah RS, Yamaoka K, Inada Y and Taniguchi N (1990). Effects of triploidy on tissue structure of some organs in ayu. Nip Sui Gak 56:569-575. https://doi.org/10.2331/suisan.56.569
  2. Allen SK and Jr. Stanley JG (1978). Reproductive sterility in polyploidy brook trout (Salvelinus fontinalis). Trans Amer Fish Soc 107:473-478. https://doi.org/10.1577/1548-8659(1978)107<473:RSIPBT>2.0.CO;2
  3. Benfey TJ (1999). The physiology and behavior of triploid fishes. Fish Sci 7:39-67. https://doi.org/10.1080/10641269991319162
  4. Cassani JR, Maloney DR, Allaire HP and Kerby JH (1990). Problems associated with tetraploid induction and survival in grass carp (Ctenopharyngodon idella). Aquaculture 88:273-284. https://doi.org/10.1016/0044-8486(90)90154-F
  5. Choi GC, Kim DS, Jo J-Y and Kim JM (1992). Induced breeding and indoor culture of the catfish (Silurus asotus) (Teleostomi: Siluridae). J Aquaculture 5:117-126.
  6. Dean PN and Jett J (1974). Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol 60:523-527. https://doi.org/10.1083/jcb.60.2.523
  7. Don J and Avtalion RR (1986). The induction of triploidy in Oreochromis aureus by heat shock. Theor Appl Genet 72:186-192. https://doi.org/10.1007/BF00266991
  8. Estevam J, Danaee H, Liu R, Ecsedy J, Trepicchio W, Wyant T (2011) Validation of a flow cytometry based $G_2M$ delay cell cycle assay for use in evaluating the pharmacodynamic response to Aurora A inhibition. J Immuno Meth 363:135-142. https://doi.org/10.1016/j.jim.2010.09.021
  9. Hulata G (2001). Genetic manipulation in aquaculture: a review of stock improvement by classical and modern technologies. Genetica 111:155-173. https://doi.org/10.1023/A:1013776931796
  10. Kim DS, Cho HJ, Park I-S, Choi GC and Nam YK (2001). Cytogenetic traits and gonad development of induced triploidy in far eastern catfish (Silurus asotus). Gene & Genomics 23:55-62.
  11. Kim DS, Choi GC and Park I-S (1990). Triploidy production of nile tilapia (Oreochromis niloticus). J Aquaculture 3:135-144.
  12. Kim DS, Jo JY and Lee TY (1994). Induction of triploidy in mud loach (Misgurnus mizolepis) and its effect on gonad development and growth. Aquaculture 120:263-270. https://doi.org/10.1016/0044-8486(94)90083-3
  13. Komaru A, Uchimura Y, Ieyama H and Wada KT (1988). Detection of induced triploid scallop (Chlamys nobilis) by DNA microflurometry with DAPI staining. Aquaculture 69:201-209. https://doi.org/10.1016/0044-8486(88)90329-8
  14. Maxime V (2008). The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish Fisher 9:67-78. https://doi.org/10.1111/j.1467-2979.2007.00269.x
  15. Murray JD, Anderson GB, Oberbauer AM and McGlough-lin MN (1999). Transgenic animals in agriculture. In Comparison of traditional breeding and transgenesis in farmed fish with implications for growth enhancement and fitness. Dunham R.A., Devlin R.H., Eds., CAB International, New York, NY, U.S.A, pp. 209-229.
  16. Nunez R (2001). DNA measurement and cell cycle analysis by flowcytometry. Curr Issues Mol Biol 3:67.
  17. Park I-S and Kim DS (2000). Comparison of some tissue in diploid and triploid hybrid between mud loach (Misgurnus mizolepis) and cyprinid loach (M. anguillicaudatus). Dev Reprod 4:19-28.
  18. Park I-S and Kim H-B (1994). Induction of triploid cherry salmon (Oncorhynchus masou). J Aquaculture 7:176-192.
  19. Park I-S and Park KY (1994). Haematological and physiological characteristics of diploid and triploid in cherry salmon (Oncorhynchus masou). J Aquaculture 7:193-201.
  20. Park I-S, Im JH and Hur JW (2004). Morphometric characteristics of catfish (Siluridae) in Korea. Kor J Ichthyol 16:223-228.
  21. Park I-S, Kim CH and Kim DS (1999). Karyotypes and cellular DNA contents of two species in the genus Lateolabrax from Korea. Fish Sci 65:488-489.
  22. Park I-S, Kim H-B, Son JK and Kim DS (1994). Induction of triploid red seabream (Pagrus major). Kor J Ichthyol 6:71-78.
  23. Park I-S, Lee C-K, Im JH, Kim JH and Kim SU (1998). Effect of starvation on the growth and hepatocyte nuclear size of larval rockfish (Sebastes schlegelii) and larval spotted sea bass (Lateolabrax sp.). J Aquaculture 11:345-352.
  24. Phillips RB, Zajicek KD, Ihssen PE and Johnson O (1986). Application of silver staining to the identification of triploid fish cells. Aquaculture 54:313-319. https://doi.org/10.1016/0044-8486(86)90275-9
  25. Seol DW, Im S-Y, Hur JW, Park MO, Kim DS, Jo J-Y and Park I-S (2008). Haematological parameters and respiratory function in diploid and triploid far eastern catfish (Silurus asotus). Genes & Genomics 30:205-213.
  26. Swarup H (1959). Effect of triploidy on the body size, general organization and cellular structure in Gasterosteus aculeatus. J Genet 56:143-155. https://doi.org/10.1007/BF02984741
  27. Takashi H (1982). An Altas of Fish Histology. In Sensory Organs. Fumio T., Ed., Kodansha Ltd, Tokyo, Japan, pp. 42-53.
  28. Tave D (1993). Growth of triploid and diploid bighead carp (Hypophthalmichthys nobilis). J Appl Aquacult 2: 13-25. https://doi.org/10.1300/J028v02n02_02
  29. Thorgaard GH (1986). Ploidy manipulation and performance. Aquaculture 57:57-64. https://doi.org/10.1016/0044-8486(86)90180-8
  30. Thorgaard GH, Rabinovitch PS, Shen MW, Gall GAE, Propp J and Utter FM (1982). Triploid rainbow trout identified by flow cytometry. Aquaculture 29:305-309. https://doi.org/10.1016/0044-8486(82)90144-2
  31. Tiwary BK, Kirubaran R and Ray AK (2004). The biology of triploid fish. Rev Fish Biol Fish 14:391-402. https://doi.org/10.1007/s11160-004-8361-8
  32. Ueno K (1984). Induction of triploid carp and their haematological characteristics. Jpn J Genet 59:585-591. https://doi.org/10.1266/jjg.59.585
  33. Vanparys C, Maras M, Lenjou M, Robbens J, Van Bock-staele D, Blust R, De Coen W (2006). Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells. Toxicol in Vitro 20:1238-1248. https://doi.org/10.1016/j.tiv.2006.05.002
  34. Wolters WR, Chrisman CL and Libey GS (1982). Erythro-cyte nuclear measurements of diploid and triploid channel catfish, Ictalurus punctatus (Rafinicsque). J Fish Biol 20:253-258. https://doi.org/10.1111/j.1095-8649.1982.tb04706.x
  35. Xoana T, Diego R, Lorena DP, Antonio R, Alicia F, Paulino M and Ana V (2012). Comparative expression analysis in mature gonads, liver and brain of turbot (Scophthalmus maximus) by cDNA-AFLPS. Gene 492:250-261. https://doi.org/10.1016/j.gene.2011.10.020
  36. Yamashita Y (1993). Histological change in the muscles of diploid and triploid ayu during growth and maturation. Bull JPN Soc Sci Fish 59:1,151-1,154. https://doi.org/10.2331/suisan.59.1151
  37. Yang WS, Gil HY, Yoo GW and Park I-S (2015). Identification of skeletal deformities in far eastern Catfish, Silurus asotus under indoor aquaculture condition. Dev Reprod 3:153-161.